FastQCFastQC Report
Thu 26 May 2016
SRR1926484_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926484_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences806642
Sequences flagged as poor quality0
Sequence length25
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTAAT450.001225726317.7331031
GTCCTAC4800.015.2393851
GTCTTAT708.693777E-515.1998011
CCCCTAA550.0046778514.5089021
TAGGACC1850.014.4861464
GATATAG852.8630751E-514.08217051
CAATAAT955.247637E-613.9998181
CTGTAGG3850.013.8180031
GTAGGAC4000.013.3793273
TCCTACA5200.013.3699092
TGTAGGA4300.013.315042
GTATTAT700.001496911213.2998271
GTATTAA1008.915133E-613.2998271
TTTATCC500.0091745613.1062793
TATAGAC500.0091745613.1062793
TAGACAG902.6934467E-612.7166625
CCCCTGT1051.4734564E-512.6665021
CCTACAG5750.012.5364423
AGGACGG852.0957303E-512.2406395
GGACGGG600.002992513612.1293826
Produced by FastQC (version 0.11.5)