FastQCFastQC Report
Thu 26 May 2016
SRR1926484_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926484_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences806642
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA11010.13649177702128082No Hit
GTCCTACAGTGGACATTTCTAAATT10950.1357479526233447No Hit
GTACATGGGAAGCAGTGGTATCAAC9420.11678043047597324No Hit
CCCATGTACTCTGCGTTGATACCAC9350.11591263534504774No Hit
GTCCTAAAGTGTGTATTTCTCATTT8120.10066423518735697No Hit
CTTTAGGACGTGAAATATGGCGAGG8110.10054026445436762No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGTG350.002170446616.2840637
GTATTAG801.2374221E-714.29474451
GTACTAG400.005167548514.29474451
GTCTTAC500.001463457713.3417621
CTTATAC603.9647587E-412.712783
TAGGACC3050.012.5020344
TAGGACG5100.012.3365674
GTAGGAC6600.012.279393
GTCCTAA3050.012.1856841
GTCCTAC6700.011.805611
TGTAGGA6800.011.7787512
AGGACGT5050.011.7036385
TTAGGAC3400.011.4975873
ATAGGAC1001.8431892E-611.4415023
TGGCGAG6800.011.42844618
GGACGTG5200.011.3660336
GACGTGA2950.011.2700447
TAAAGGT1201.2124292E-711.1216014
CTGTAGG7200.011.11813451
GTACTAT600.00573602411.11813451
Produced by FastQC (version 0.11.5)