FastQCFastQC Report
Thu 26 May 2016
SRR1926483_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926483_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences835496
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGGA5600.018.508142
CTGTAGG5800.016.7372551
GTACTGA500.00248258715.9577121
GTCCTAC7050.015.4672881
GTAGGAC6050.015.3105243
TAAGACT506.071907E-414.9865854
TTTAGGA3550.014.9821282
TAGGACC2650.014.946194
TAAGGGT651.767791E-514.8218974
GTGCTAG903.0007322E-614.775661
ATAGACC450.004634930314.5299863
GCATAAG550.004682261514.5070111
TCCTACA8250.014.3814462
TACTGGA954.047557E-614.3553452
TAGGACG5500.013.8187994
AGGACGT5500.013.7872885
CTTTAGG3400.013.6892141
GGACGTG5400.013.6556566
TATAAGG600.007052900313.6375772
CTCTAGG904.9081762E-513.2980941
Produced by FastQC (version 0.11.5)