FastQCFastQC Report
Thu 26 May 2016
SRR1926483_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926483_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences835496
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA17640.2111320700518016No Hit
GTCCTACAGTGGACATTTCTAAATT16760.20059940442563462No Hit
GTCCTACAGTGTGCATTTCTCATTT13740.1644532110267434No Hit
CTTTAGGACGTGAAATATGGCGAGG12230.1463801143272978No Hit
GTCCTAAAGTGTGTATTTCTCATTT12090.1447044629776803No Hit
CTGTAGGACCTGGAATATGGCGAGA11610.1389593726361347No Hit
CCCATGTACTCTGCGTTGATACCAC9760.11681683694476094No Hit
GTACATGGGAAGCAGTGGTATCAAC9420.11274739795283281No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGAC456.5826264E-414.8290083
CGCCACC707.3816736E-613.54832514
GTGCGCC655.537462E-513.13066711
TGTAGGA11100.012.8869992
AACCATC604.0976482E-412.6633087
CGAAATC604.1476835E-412.64510313
TAGGACC4700.012.3725324
GTAGGAC11350.012.26262953
CTGTAGG11250.012.2014221
CCGGTTT550.00310577712.07032512
GTCCTAC10750.011.7935251
TAGGACA3800.011.7907344
TTAGACT657.787116E-411.73284154
CGGTTTC658.1319915E-411.67240313
GGCGAGG4150.011.64575519
AGGACCT7000.011.5757065
GGGCTAC752.0071893E-411.4388321
GCGCCAC752.104663E-411.38059313
GGACCTG6700.011.2396496
CTACACT1701.0913936E-1111.2152174
Produced by FastQC (version 0.11.5)