##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926483_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 835496 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.989594205118877 31.0 28.0 31.0 16.0 34.0 2 28.501028131792374 31.0 28.0 33.0 22.0 34.0 3 28.874304604689907 31.0 28.0 33.0 23.0 34.0 4 32.617968248800715 35.0 32.0 37.0 26.0 37.0 5 32.267850474448714 35.0 32.0 37.0 24.0 37.0 6 32.31300808142708 35.0 33.0 37.0 24.0 37.0 7 32.15914259314228 35.0 33.0 37.0 21.0 37.0 8 32.09891609295556 35.0 33.0 37.0 19.0 37.0 9 33.32467300860806 37.0 33.0 39.0 18.0 39.0 10 33.209583289447224 37.0 33.0 39.0 18.0 39.0 11 33.41132572747206 37.0 33.0 39.0 21.0 39.0 12 33.20996390168236 37.0 33.0 39.0 18.0 39.0 13 33.321683167842814 37.0 33.0 39.0 19.0 39.0 14 33.96731522353189 38.0 33.0 40.0 17.0 41.0 15 34.08203989007727 38.0 33.0 40.0 17.0 41.0 16 34.060487423039724 38.0 33.0 40.0 17.0 41.0 17 33.968617444009304 38.0 33.0 40.0 17.0 41.0 18 33.947550915863154 38.0 33.0 40.0 17.0 41.0 19 33.98263785823032 39.0 33.0 40.0 18.0 41.0 20 33.954142210136254 39.0 33.0 40.0 18.0 41.0 21 33.885616448193645 39.0 33.0 40.0 16.0 41.0 22 33.804883566169075 39.0 33.0 40.0 10.0 41.0 23 33.452953694571846 39.0 33.0 40.0 8.0 41.0 24 33.52404679376083 39.0 33.0 40.0 2.0 41.0 25 33.316123596043546 38.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9466785458940237 1101 2 1.1332847261125067 1101 3 0.9934605663614242 1101 4 0.7762776332071013 1101 5 1.028794351459986 1101 6 0.6487505380837533 1101 7 0.9152739275460888 1101 8 0.8616976988326925 1101 9 1.298843210685071 1101 10 1.4939070524155227 1101 11 1.1260280480788794 1101 12 1.2601872562396252 1101 13 1.034587261000695 1101 14 1.2547475781146034 1101 15 1.235119205068699 1101 16 1.2944357465121996 1101 17 1.2917422845064834 1101 18 1.2790431963847055 1101 19 1.140645511072023 1101 20 1.2076233011731787 1101 21 1.3996926421331821 1101 22 1.268285527811713 1101 23 1.5241559237430096 1101 24 1.3426679926800702 1101 25 1.406412825597819 1102 1 0.6916178527717101 1102 2 0.6859913263158965 1102 3 0.5318214352742849 1102 4 0.5448199238931721 1102 5 0.6300202543546973 1102 6 0.11317564371553601 1102 7 0.4658563147780299 1102 8 0.41925428430968736 1102 9 0.6557211779609844 1102 10 0.7460965616653681 1102 11 0.3026174335899583 1102 12 0.40596096619818667 1102 13 0.22738911041358278 1102 14 0.40928441910843816 1102 15 0.48134776612396735 1102 16 0.45073975487012774 1102 17 0.4239816041655686 1102 18 0.613958922507102 1102 19 0.616934163227171 1102 20 0.4805098782959618 1102 21 0.5344553619408288 1102 22 0.4505343872107659 1102 23 0.5899375248262828 1102 24 0.4724816250610999 1102 25 0.6614327664329096 1103 1 0.4355706296830988 1103 2 0.32323093902817135 1103 3 0.3206315010388714 1103 4 0.20169268832852794 1103 5 0.318945789728879 1103 6 0.08257287908109134 1103 7 0.17693343387762894 1103 8 0.33230367743062317 1103 9 0.5817741745300751 1103 10 0.850911777109097 1103 11 0.4995428893299305 1103 12 0.5977566513510482 1103 13 0.40396470650885163 1103 14 0.7925141173611294 1103 15 0.5329374641112068 1103 16 0.5583786668013175 1103 17 0.4751333139784961 1103 18 0.6931783744909907 1103 19 0.7835853454634361 1103 20 0.8076405912212579 1103 21 0.7718449524218869 1103 22 0.6760750703395928 1103 23 0.987673922307188 1103 24 0.8530269866999731 1103 25 0.8926606596434326 1104 1 0.606377879373845 1104 2 0.5681891427242398 1104 3 0.3953063145486375 1104 4 0.20915136059302597 1104 5 0.3327769926377542 1104 6 0.06170190408390397 1104 7 0.24489928461046873 1104 8 0.39304795152440875 1104 9 0.632707562842107 1104 10 0.7517130291239411 1104 11 0.3503835010928711 1104 12 0.6428760425869413 1104 13 0.41482237416263956 1104 14 0.801610904855977 1104 15 0.5189066510512674 1104 16 0.4713748807684084 1104 17 0.5256890361361641 1104 18 0.43065822604851434 1104 19 0.41142299587389886 1104 20 0.5560155959069419 1104 21 0.6107305092686062 1104 22 0.5460651366888882 1104 23 0.6279005219998055 1104 24 0.52236293152135 1104 25 0.6763516668781264 1105 1 0.7125588511405887 1105 2 0.5437347720355596 1105 3 0.6010735880983376 1105 4 0.37958466000122115 1105 5 0.5787314198683262 1105 6 0.22213671127602908 1105 7 0.3257494862765711 1105 8 0.2611517247537094 1105 9 0.7631026022329621 1105 10 0.8618952825070281 1105 11 0.5608371555841813 1105 12 0.7232390415691938 1105 13 0.5876879175302747 1105 14 1.0369957667052745 1105 15 0.8846611084375269 1105 16 0.7525011953675289 1105 17 0.5190557291890485 1105 18 0.5770923170432738 1105 19 0.7359160035259009 1105 20 0.596035950990057 1105 21 0.6995333288245646 1105 22 0.7360713562976144 1105 23 0.9915959119504265 1105 24 0.6446489204391384 1105 25 0.8890839598405478 1106 1 0.9080860889238771 1106 2 1.0794202129365438 1106 3 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0.5354938264619733 1107 16 0.5987984503826453 1107 17 0.45498156395809985 1107 18 0.629646768343342 1107 19 0.7936630960146118 1107 20 0.5793037928259679 1107 21 0.8557454024579414 1107 22 0.7835562281860646 1107 23 0.8739912882224203 1107 24 0.648760881195166 1107 25 0.8121816652334815 1108 1 1.3328197432068656 1108 2 1.3248780151197437 1108 3 1.1341904766745934 1108 4 0.8601863708209407 1108 5 -0.37756813145486134 1108 6 0.4811109480910716 1108 7 0.9563613327269067 1108 8 1.0028183213584114 1108 9 1.3966374337713034 1108 10 1.5157420112205742 1108 11 1.2082087576957576 1108 12 1.260262585191171 1108 13 1.136180604462595 1108 14 1.587145652597691 1108 15 1.33543604045407 1108 16 1.3863293529901242 1108 17 1.3056572662550963 1108 18 1.12191562544389 1108 19 1.170746234048444 1108 20 1.289597536856597 1108 21 1.3696713322080925 1108 22 1.4949993522172065 1108 23 1.7306552693794686 1108 24 1.6461048080261236 1108 25 1.5453380175958884 1109 1 0.4433989663949305 1109 2 -0.7199382704031727 1109 3 0.26635992310224665 1109 4 0.5405183044599724 1109 5 -1.042958083097325 1109 6 0.21616804605004347 1109 7 0.5998703270815113 1109 8 0.6762637222401793 1109 9 1.2043403219748612 1109 10 -1.2985134336025226 1109 11 0.2852104984198647 1109 12 0.5761920509029537 1109 13 0.7360022078424748 1109 14 1.1367608900059594 1109 15 1.0393588340034512 1109 16 0.945981355374883 1109 17 1.1047876402347683 1109 18 1.0208349412252247 1109 19 1.0281775251284273 1109 20 1.259750374641719 1109 21 1.1567804053185 1109 22 1.070779273903021 1109 23 1.1685858501851314 1109 24 1.1175700892285079 1109 25 1.3030148685413252 1110 1 0.6710962690922315 1110 2 -0.23320000866491242 1110 3 0.45400977325209624 1110 4 0.7584378848795481 1110 5 0.8747492096099663 1110 6 0.5355112028932041 1110 7 0.7388938036049879 1110 8 0.9452072786837391 1110 9 1.248233928368471 1110 10 0.8506499030608126 1110 11 0.698384824094191 1110 12 1.0298633795742802 1110 13 0.9075931169333842 1110 14 1.2144581927032618 1110 15 1.1081525402971621 1110 16 0.8629643723579008 1110 17 1.167075352522481 1110 18 1.1552755006657875 1110 19 1.0902029996539042 1110 20 1.1197653596567037 1110 21 1.1784587269968227 1110 22 1.142195357819105 1110 23 1.2673495864488729 1110 24 1.1672079513663718 1110 25 1.4635043790308373 1111 1 0.1065673643444498 1111 2 0.1977136740955565 1111 3 0.44588123967896465 1111 4 0.3458495156564325 1111 5 0.6135206980394585 1111 6 0.3145589677138787 1111 7 0.5524426350744776 1111 8 0.7677207433312674 1111 9 1.1036193090616493 1111 10 1.057905028236597 1111 11 0.4966427904780133 1111 12 0.6471038682238017 1111 13 0.34538214049531746 1111 14 0.6591579525877052 1111 15 0.5918093759375793 1111 16 0.587838813921806 1111 17 0.32160263098956676 1111 18 0.43234972603002575 1111 19 0.4100522338670203 1111 20 0.3241266618292258 1111 21 0.4395858351827542 1111 22 0.354196352720237 1111 23 0.6904370325185312 1111 24 0.5538035718881247 1111 25 0.46572664377020345 1112 1 0.03614115328605294 1112 2 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1.0306039892907108 2202 25 1.172598115483062 2203 1 0.1740246839609476 2203 2 0.40790465781288887 2203 3 0.14156152498295071 2203 4 -0.17380499661706494 2203 5 0.13687762845480478 2203 6 0.1115093341152118 2203 7 0.3720362830161221 2203 8 0.4268206901478919 2203 9 0.6836641697688606 2203 10 0.6376266079479649 2203 11 0.4733665587774212 2203 12 0.7455854684306971 2203 13 0.5190204955249413 2203 14 0.9008593537312919 2203 15 0.9125580850012085 2203 16 0.7567626139173385 2203 17 0.8689202737703852 2203 18 0.7420981307719998 2203 19 0.6888337221652208 2203 20 0.6742506753360473 2203 21 0.8172144985884202 2203 22 0.77435275266307 2203 23 1.142955075487194 2203 24 1.03324241441576 2203 25 0.9529383281662831 2204 1 0.30151182510020647 2204 2 0.36985522993535014 2204 3 0.20685455879830172 2204 4 0.1545195283545624 2204 5 0.3265083509425679 2204 6 0.4490497970005123 2204 7 0.43657479559878 2204 8 0.5482918718157208 2204 9 0.8604747680974612 2204 10 0.9254495110357794 2204 11 0.6070785000864447 2204 12 0.6993171485541154 2204 13 0.4663320083736906 2204 14 0.8849706186659247 2204 15 1.0480855930264212 2204 16 0.9699384409433449 2204 17 0.8386686880968597 2204 18 1.0128600961650776 2204 19 1.0282133305552321 2204 20 0.9164284122439241 2204 21 0.9148138410770557 2204 22 0.9941003248899491 2204 23 1.202649831227788 2204 24 1.2940810782395005 2204 25 1.2705816619375057 2205 1 0.7430534840192244 2205 2 0.6655272824408307 2205 3 0.6763873956297175 2205 4 0.590016687154808 2205 5 0.5151836765884568 2205 6 0.546209308992097 2205 7 0.3963669480689376 2205 8 0.45603495298053076 2205 9 0.9442908281047409 2205 10 0.8170724424137106 2205 11 0.6336034819146903 2205 12 0.7282313716347844 2205 13 0.5264411545724812 2205 14 1.0646121123709804 2205 15 0.7919932724953185 2205 16 0.8897077997583551 2205 17 0.9785187054901385 2205 18 0.7514399144386132 2205 19 0.9515080546514696 2205 20 1.189644888800359 2205 21 0.9262133376949322 2205 22 0.9044937690005668 2205 23 1.1327190395975606 2205 24 0.9635010144137084 2205 25 1.1773452031213338 2206 1 0.38546533356149837 2206 2 0.5345588190137569 2206 3 0.4656325526103444 2206 4 0.5023490171674396 2206 5 0.7837677128168465 2206 6 0.597235584056456 2206 7 0.808519768822137 2206 8 0.8183386229573699 2206 9 1.0831625268241254 2206 10 0.9836548406749728 2206 11 0.7878462644574356 2206 12 0.8997943837738234 2206 13 0.8625276947136484 2206 14 0.9405378578610879 2206 15 0.6164007383249199 2206 16 0.9571392022040754 2206 17 0.8976315698522441 2206 18 0.8006188680532418 2206 19 0.7997577735373369 2206 20 0.8856514441800627 2206 21 0.9538103626691026 2206 22 0.8959392091581506 2206 23 1.2087554751493457 2206 24 0.9932890179284044 2206 25 1.0950733444675365 2207 1 -0.8227770217959822 2207 2 0.15578861465207083 2207 3 0.36024085560258 2207 4 0.4287019491734654 2207 5 0.3480296605023625 2207 6 0.4017464601732357 2207 7 0.4092204918421274 2207 8 0.6661758623388891 2207 9 0.8055870230349953 2207 10 0.7104766434845686 2207 11 0.4475863688181292 2207 12 0.47647646797841503 2207 13 0.3199509083956471 2207 14 -4.525902938702643 2207 15 -1.6342856185589127 2207 16 -0.7628471295730037 2207 17 -0.6320166003008154 2207 18 -0.0314690980191088 2207 19 0.11902045085941637 2207 20 0.2376027461508059 2207 21 0.4548703750502625 2207 22 0.43921028319223865 2207 23 0.7281783099641572 2207 24 0.09946971944903282 2207 25 -0.013366511272884907 2208 1 0.5009267250945655 2208 2 0.9457053307873018 2208 3 0.7590696872963854 2208 4 0.7110411409984323 2208 5 0.8650017955656821 2208 6 0.8873705414712916 2208 7 0.9046508330338909 2208 8 1.043973486434318 2208 9 1.3208410087880509 2208 10 1.251259059529346 2208 11 0.9630664965883682 2208 12 1.103085079749107 2208 13 0.9377249642527303 2208 14 1.247824305756879 2208 15 1.0672033852855023 2208 16 1.2046237546422844 2208 17 0.8670143458208486 2208 18 1.1285572969944582 2208 19 0.6388617627657922 2208 20 0.9617306859970327 2208 21 0.9837034822415802 2208 22 1.2550741456978969 2208 23 1.581532278756562 2208 24 1.3532328639537852 2208 25 1.3964143076438944 2209 1 -0.2868133888174249 2209 2 0.08152940606450443 2209 3 0.3461725654148857 2209 4 0.500540826982494 2209 5 0.4100292448900049 2209 6 0.2985175658995658 2209 7 0.7844869491981328 2209 8 0.5302447637212211 2209 9 0.6609473410818865 2209 10 0.2875238399347495 2209 11 0.3686613943707613 2209 12 0.35053370024460406 2209 13 0.38963179897206146 2209 14 0.667983046228116 2209 15 0.6469038790485016 2209 16 0.5884919978855265 2209 17 0.7728650583121848 2209 18 0.7329797783700656 2209 19 0.528765338216246 2209 20 0.4364036762950221 2209 21 0.3778476263857229 2209 22 0.47068398630773345 2209 23 0.8051874242867072 2209 24 0.6636484478068709 2209 25 0.7574500729765319 2210 1 -0.38794654126918005 2210 2 -0.37510322305565325 2210 3 0.4614907455940802 2210 4 0.6797563383434095 2210 5 1.0795276813164492 2210 6 0.9352320807323977 2210 7 0.9165705103622344 2210 8 0.9962163551382872 2210 9 1.3389885052242576 2210 10 1.2570293397324832 2210 11 1.0545093125779132 2210 12 0.9419947264882396 2210 13 0.6296333005558878 2210 14 1.099880539422827 2210 15 1.1511214032232573 2210 16 1.2620936109026744 2210 17 1.2773789477155972 2210 18 1.3732185697136288 2210 19 1.2344089718712823 2210 20 1.3743546762559973 2210 21 1.4867327053349513 2210 22 1.4321649035976307 2210 23 1.5121162951500011 2210 24 1.4842857417157234 2210 25 1.4858510985977986 2211 1 -1.6482629581046417 2211 2 -0.8047753192885061 2211 3 -0.33235998015754475 2211 4 -0.011108034561402746 2211 5 0.25901959283066844 2211 6 -0.14829232737638876 2211 7 0.029910065160088095 2211 8 0.11034647818262755 2211 9 0.16385294895599856 2211 10 0.1578357193439004 2211 11 0.03152746871339218 2211 12 -0.024597904418143912 2211 13 -0.07058322037521947 2211 14 0.20754446262647974 2211 15 -0.05271402490810573 2211 16 -0.06086274524108859 2211 17 0.13842947076253154 2211 18 0.06222348620019602 2211 19 -0.028053201807338723 2211 20 -0.12095752837199569 2211 21 0.13153413304633688 2211 22 0.10149938479283094 2211 23 0.08792879730099656 2211 24 0.2368982330930436 2211 25 0.21485477810691833 2212 1 0.8937345164537263 2212 2 -0.010752714261897722 2212 3 0.24280805983636 2212 4 0.3878962493932647 2212 5 0.563441697933456 2212 6 0.5055161057394315 2212 7 0.5873571483782953 2212 8 0.6063096449521552 2212 9 0.7718269405223666 2212 10 0.3906055194001965 2212 11 0.4213095008934076 2212 12 0.6926765811328721 2212 13 0.5407673228013152 2212 14 0.477204302681848 2212 15 0.4683930378070329 2212 16 0.030365605810793284 2212 17 0.6282396494801361 2212 18 0.29782188417158295 2212 19 0.45912669588387445 2212 20 0.585835083291002 2212 21 0.6049211944187007 2212 22 0.7543053880103159 2212 23 1.1526462086458622 2212 24 0.841787983879982 2212 25 0.2642269037386029 2213 1 0.26366722754554317 2213 2 0.07968357833632211 2213 3 -0.4275341729977349 2213 4 -1.3212275477270587 2213 5 -2.0656676284539266 2213 6 -0.8982636497051821 2213 7 -0.459814546868067 2213 8 -0.34302253072254274 2213 9 -0.7987974716041037 2213 10 -0.938273722333399 2213 11 -0.6448072779214371 2213 12 -0.8586872854469689 2213 13 -0.7144993004531557 2213 14 -7.912880351105869 2213 15 -3.4511728402046913 2213 16 -2.0492610492792878 2213 17 -1.5293321615320963 2213 18 -5.365160533887895 2213 19 -3.2981557004695006 2213 20 -5.713894314002971 2213 21 -2.712963184425085 2213 22 -2.0616905601844593 2213 23 -4.7544603683022615 2213 24 -2.5370673811442543 2213 25 -2.2162159006894413 2214 1 0.32997738797335074 2214 2 0.1061006906357882 2214 3 -1.6572489680066447 2214 4 -2.3913523249106596 2214 5 -0.5785375036767455 2214 6 -0.5665167691347719 2214 7 -0.8697075949964095 2214 8 -0.7214360779596198 2214 9 -5.696801036666496 2214 10 -1.7196997472888356 2214 11 -1.2006361549267837 2214 12 -1.231325431614529 2214 13 -1.578420869080606 2214 14 -2.963094254849185 2214 15 -2.3662530541084337 2214 16 -1.6544482150546855 2214 17 -2.148868703421577 2214 18 -1.3970321916419053 2214 19 -4.060776021325118 2214 20 -2.232682192790847 2214 21 -3.8690237904856914 2214 22 -6.659622823999076 2214 23 -6.930342721243438 2214 24 -5.978386454228033 2214 25 -3.7034886279621695 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 32486.0 3 16.0 4 596.0 5 4084.0 6 4883.0 7 3844.0 8 2916.0 9 2340.0 10 2164.0 11 1973.0 12 1793.0 13 1724.0 14 1753.0 15 1722.0 16 1786.0 17 1914.0 18 2092.0 19 2595.0 20 3251.0 21 4152.0 22 5552.0 23 6833.0 24 8017.0 25 9440.0 26 10692.0 27 11981.0 28 13599.0 29 16277.0 30 20030.0 31 24724.0 32 31505.0 33 40344.0 34 52589.0 35 68519.0 36 92395.0 37 133644.0 38 211271.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.133774732890174 19.060961775053936 8.730918373825574 31.074345118230312 2 13.517121487673558 21.333226377662868 39.09417327058068 26.055478864082886 3 18.579461988528138 24.057510167480583 28.587232014903126 28.775795829088153 4 11.757212519859348 17.318069222471383 37.23608169539862 33.68863656227065 5 12.85836737234771 38.62047416785983 35.396544541227506 13.12461391856495 6 32.33159762687998 38.115416869913325 17.150694082338326 12.402291420868366 7 28.69629022163714 31.310232043986925 21.279251282272625 18.71422645210331 8 25.097995014215122 38.644629129789884 19.404151942933268 16.853223913061726 9 26.05748903089337 15.613244709107327 19.301190863054945 39.02807539694436 10 14.112392531106424 30.41246986322032 34.14224799427311 21.33288961140014 11 34.84404717305204 22.68811708760385 23.90765993558171 18.5601758037624 12 23.2138154902402 25.117299525535387 31.619403735391423 20.04948124883299 13 29.276262244223787 22.117161542365256 25.186116989189657 23.4204592242213 14 20.17285405617757 22.91058826911192 27.310270156325604 29.606287518384907 15 22.527768468425336 32.02877387848901 22.570139321108822 22.873318331976826 16 21.620214439090248 28.86321907593461 28.090252094000828 21.426314390974312 17 21.26944723064733 29.352959905011094 27.873186939983196 21.504405924358384 18 21.126312135684106 28.34368678707793 29.748764168671528 20.781236908566434 19 23.079952171607697 27.472480122371746 27.065172767499675 22.382394938520886 20 23.123675926701694 28.12014506457289 27.923613687776033 20.832565320949385 21 22.239896396622836 27.3195259331605 26.872724400175223 23.567853270041436 22 22.378443463523464 29.09409500464395 26.932863831783756 21.594597700048833 23 21.712491741432636 28.591758667905054 27.562549671093578 22.133199919568735 24 22.785082837219655 27.438258084438665 28.38871374300713 21.387945335334543 25 22.14567412132927 28.154926121640468 27.775510326213805 21.923889430816462 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 140.0 1 140.0 2 146.5 3 153.0 4 153.0 5 153.0 6 259.5 7 366.0 8 366.0 9 366.0 10 672.0 11 978.0 12 978.0 13 978.0 14 1711.0 15 2444.0 16 2444.0 17 2444.0 18 4691.5 19 6939.0 20 6939.0 21 6939.0 22 11788.0 23 16637.0 24 16637.0 25 16637.0 26 25511.5 27 34386.0 28 34386.0 29 34386.0 30 47376.0 31 60366.0 32 60366.0 33 60366.0 34 71440.5 35 82515.0 36 82515.0 37 82515.0 38 90973.0 39 99431.0 40 99431.0 41 99431.0 42 105226.0 43 111021.0 44 111021.0 45 111021.0 46 114674.5 47 118328.0 48 118328.0 49 118328.0 50 112704.0 51 107080.0 52 107080.0 53 107080.0 54 92837.5 55 78595.0 56 78595.0 57 78595.0 58 66542.5 59 54490.0 60 54490.0 61 54490.0 62 43612.0 63 32734.0 64 32734.0 65 32734.0 66 24908.0 67 17082.0 68 17082.0 69 17082.0 70 12270.0 71 7458.0 72 7458.0 73 7458.0 74 5157.0 75 2856.0 76 2856.0 77 2856.0 78 1965.5 79 1075.0 80 1075.0 81 1075.0 82 693.0 83 311.0 84 311.0 85 311.0 86 200.5 87 90.0 88 90.0 89 90.0 90 54.0 91 18.0 92 18.0 93 18.0 94 10.5 95 3.0 96 3.0 97 3.0 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08701418079799306 2 0.03099954996792325 3 0.028127004797150433 4 0.02956327738253684 5 0.02154408878079608 6 0.37989409883470415 7 0.1711558164252133 8 0.182526307726189 9 0.1870745042465793 10 0.016517134731943658 11 0.0022740982601951415 12 0.003351302699234945 13 0.0 14 0.06846232657008532 15 0.0019150301138485403 16 0.0016756513496174726 17 0.003590681463466013 18 0.0031119239350038777 19 0.0010772044390398039 20 7.181362926932025E-4 21 2.3937876423106753E-4 22 0.0 23 0.0 24 2.3937876423106753E-4 25 1.1968938211553377E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 835496.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.97915026301561 #Duplication Level Percentage of deduplicated Percentage of total 1 92.87308628364404 78.92275954687801 2 5.499974301863559 9.347662852815755 3 0.8293443743437967 2.1143094062144447 4 0.2723165277960629 0.9256490853873719 5 0.12990850944499494 0.5519757372285303 6 0.08677693110993204 0.4424537920892561 7 0.05776469314663502 0.34361561771634197 8 0.03541436740205673 0.24075862791432165 9 0.02933288399564524 0.22434152010421884 >10 0.1596226003714298 2.490262495680752 >50 0.013584450513202652 0.8028702511828788 >100 0.011317869425323196 2.0760083773952522 >500 7.073667924131249E-4 0.5066191231361746 >1k 8.488401508957499E-4 1.0107135662567204 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTAGGACGTGGAATATGGCAAGA 1764 0.2111320700518016 No Hit GTCCTACAGTGGACATTTCTAAATT 1676 0.20059940442563462 No Hit GTCCTACAGTGTGCATTTCTCATTT 1374 0.1644532110267434 No Hit CTTTAGGACGTGAAATATGGCGAGG 1223 0.1463801143272978 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1209 0.1447044629776803 No Hit CTGTAGGACCTGGAATATGGCGAGA 1161 0.1389593726361347 No Hit CCCATGTACTCTGCGTTGATACCAC 976 0.11681683694476094 No Hit GTACATGGGAAGCAGTGGTATCAAC 942 0.11274739795283281 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 3.590681463466013E-4 0.0 0.0 0.0 0.0 7 3.590681463466013E-4 0.0 0.0 0.0 0.0 8 3.590681463466013E-4 0.0 0.0 0.0 0.0 9 3.590681463466013E-4 0.0 0.0 0.0 0.0 10 3.590681463466013E-4 0.0 0.0 0.0 0.0 11 3.590681463466013E-4 0.0 0.0 0.0 0.0 12 3.590681463466013E-4 0.0 0.0 0.0 0.0 13 3.590681463466013E-4 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTAGAC 45 6.5826264E-4 14.829008 3 CGCCACC 70 7.3816736E-6 13.548325 14 GTGCGCC 65 5.537462E-5 13.130667 11 TGTAGGA 1110 0.0 12.886999 2 AACCATC 60 4.0976482E-4 12.663308 7 CGAAATC 60 4.1476835E-4 12.645103 13 TAGGACC 470 0.0 12.372532 4 GTAGGAC 1135 0.0 12.2626295 3 CTGTAGG 1125 0.0 12.201422 1 CCGGTTT 55 0.003105777 12.070325 12 GTCCTAC 1075 0.0 11.793525 1 TAGGACA 380 0.0 11.790734 4 TTAGACT 65 7.787116E-4 11.7328415 4 CGGTTTC 65 8.1319915E-4 11.672403 13 GGCGAGG 415 0.0 11.645755 19 AGGACCT 700 0.0 11.575706 5 GGGCTAC 75 2.0071893E-4 11.438832 1 GCGCCAC 75 2.104663E-4 11.380593 13 GGACCTG 670 0.0 11.239649 6 CTACACT 170 1.0913936E-11 11.215217 4 >>END_MODULE