FastQCFastQC Report
Thu 26 May 2016
SRR1926482_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926482_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences922018
Sequences flagged as poor quality0
Sequence length25
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCTAG754.350677E-717.7796651
CTTAGAC400.002051660816.421083
GTTCTAG1055.6365025E-815.2397131
AATACCT558.918324E-515.1563335
CTAGTAC601.2287626E-414.5965173
CTTTAGG2800.014.2872311
GTCCTAC4600.013.9145211
TATACTG755.6883346E-613.8933055
GATATAC1253.1512172E-813.8681391
TAGGACG4700.013.7111134
TCTAGTA700.001219054813.6673682
AGGACGT4050.013.6360225
GTCCTAA2500.013.3347491
GGACGTG4400.013.2538836
TCTAGGC755.509035E-513.1368643
TTAGTAC500.00903619613.1368643
GGTTCAC450.00964261713.0085438
GTAGGAC4800.013.0000233
CTCCAGA1655.293259E-1012.9306661
TCCTAAA2550.012.8634052
Produced by FastQC (version 0.11.5)