FastQCFastQC Report
Thu 26 May 2016
SRR1926482_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926482_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences922018
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT15230.16518115698392005No Hit
CTGTAGGACGTGGAATATGGCAAGA14760.16008364261869074No Hit
GTCCTACAGTGTGCATTTCTCATTT12860.13947666965287012No Hit
CCCATGTACTCTGCGTTGATACCAC11440.12407566880473049No Hit
CTGTAGGACCTGGAATATGGCGAGA11260.12212342926060012No Hit
GTACATGGGAAGCAGTGGTATCAAC11040.119737358706663No Hit
CTTTAGGACGTGAAATATGGCGAGG10610.11507367535124043No Hit
GTCCTAAAGTGTGTATTTCTCATTT10400.112796062549755No Hit
GTATCAACGCAGAGTACTTTTTTTT10390.1126876047973033No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG400.005142501614.3055255
TAGGACC3550.013.4324184
TAGATCT655.2525174E-513.2051014
GATATAC2600.012.836891
CTAGGAC1501.8189894E-1212.71883
CCTAAGG1103.5859557E-812.1426622
GTCCTAC8900.012.0003291
TGTAGGA9150.011.8867272
GTCCTAA3700.011.8555371
AGGACCT5350.011.7652915
CTCCTAC1251.727858E-811.4431711
GGACCTG5350.011.4081456
GTAGGAC9200.011.301763
TTAGACT600.005704967311.1265194
TCCTACA9950.011.1227832
AGTGATC600.005878824711.0817478
AATGCCG951.399611E-510.97038818
GGCGAGG2850.010.96979319
GGACGTG6450.010.9410966
CTAAAGT3850.010.8994484
Produced by FastQC (version 0.11.5)