FastQCFastQC Report
Thu 26 May 2016
SRR1926481_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926481_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences609635
Sequences flagged as poor quality0
Sequence length25
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAA1351.8189894E-1216.7015041
GAACAGG654.7206588E-516.3236431
TCCTACA2350.016.1758582
TCCAACG406.15085E-414.96070218
CTAGGAC1103.5652192E-1014.8345823
TAAGTAC450.00468563414.5049253
GTTCTTA550.0047618614.4686821
TCCTAAA1703.6379788E-1214.3747642
TAGACTG807.439594E-714.2555055
TTGGACG450.00526641614.2518844
CTTATAC550.001104922313.845613
CTGTAGG2050.013.5864461
TCCTATA802.2687837E-413.5761662
GTCCTAC2450.013.5336321
CACTATT450.007443401513.5268717
TAGGACC1355.0931703E-1113.4601134
GTACAGG802.7648234E-413.26295951
ACTACAC500.00941299713.0544323
TAATAGT756.8822395E-512.8266954
CTACACT756.8822395E-512.8266954
Produced by FastQC (version 0.11.5)