##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926481_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 609635 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 19.953478720873967 26.0 2.0 30.0 2.0 31.0 2 15.755283079219533 16.0 2.0 30.0 2.0 31.0 3 19.168853494303967 20.0 10.0 30.0 2.0 31.0 4 21.247843381695606 27.0 2.0 35.0 2.0 35.0 5 21.62992774364989 28.0 2.0 35.0 2.0 35.0 6 22.481860457486857 30.0 2.0 35.0 2.0 37.0 7 22.31892689888212 30.0 2.0 35.0 2.0 37.0 8 22.385609422031216 30.0 2.0 35.0 2.0 37.0 9 24.899267594544277 31.0 13.0 37.0 2.0 39.0 10 25.502984572736146 31.0 12.0 37.0 2.0 39.0 11 22.751438155617706 29.0 2.0 37.0 2.0 39.0 12 23.744771871693718 30.0 2.0 37.0 2.0 39.0 13 20.620087429363473 26.0 2.0 35.0 2.0 39.0 14 23.762863024596683 30.0 2.0 37.0 2.0 40.0 15 24.749889688092054 31.0 2.0 38.0 2.0 40.0 16 25.117163548680768 31.0 2.0 38.0 2.0 40.0 17 24.402647485790677 30.0 2.0 38.0 2.0 40.0 18 24.437819350923093 30.0 2.0 37.0 2.0 40.0 19 24.806339859096017 30.0 2.0 38.0 2.0 40.0 20 25.017275910995924 31.0 2.0 38.0 2.0 40.0 21 22.929287196437212 30.0 2.0 37.0 2.0 40.0 22 23.82074355967095 30.0 2.0 37.0 2.0 40.0 23 23.77660895453837 30.0 2.0 37.0 2.0 40.0 24 22.969653973279094 30.0 2.0 38.0 2.0 40.0 25 22.740080539995244 30.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 4.761808152367966 1101 2 9.163417364918795 1101 3 6.256489656944336 1101 4 7.918847376044404 1101 5 7.4841727568667125 1101 6 7.078409573111653 1101 7 6.737607580255094 1101 8 5.049016649519331 1101 9 5.00896806894092 1101 10 4.936294042756025 1101 11 7.377053991941143 1101 12 5.082266211517098 1101 13 9.364064999305437 1101 14 7.040365334680491 1101 15 6.263436781075761 1101 16 5.770724151803524 1101 17 1.9463215050525484 1101 18 4.275973511728491 1101 19 4.736570167159755 1101 20 2.763168125376321 1101 21 -0.23260997108434367 1101 22 3.649447540268497 1101 23 5.093448560283264 1101 24 6.3289494157698165 1101 25 6.805323789012995 1102 1 4.7488512339907984 1102 2 9.617343672118592 1102 3 6.274970396801635 1102 4 7.4662915624315325 1102 5 7.393720180129531 1102 6 6.319726293133414 1102 7 6.226314085391827 1102 8 6.3632833831414075 1102 9 5.592897723911314 1102 10 4.989978682766068 1102 11 7.345532639441821 1102 12 5.02215803667978 1102 13 8.954156644525451 1102 14 6.88754483795503 1102 15 4.643811522146628 1102 16 5.199202171597218 1102 17 6.07491251210379 1102 18 5.941290608374445 1102 19 4.527920364887457 1102 20 5.077999795286139 1102 21 4.037194184335906 1102 22 5.089951379742896 1102 23 5.374389283820182 1102 24 6.598042169938836 1102 25 6.381192640099972 1103 1 4.107806484719319 1103 2 8.275931965458367 1103 3 5.811858865396971 1103 4 7.256802921492998 1103 5 6.771389058476821 1103 6 6.415776790033462 1103 7 6.53177270503587 1103 8 6.1417684076137995 1103 9 5.182943619282202 1103 10 4.35863209305424 1103 11 5.043072894256209 1103 12 4.3454420140730114 1103 13 8.058700097796631 1103 14 6.109752783901623 1103 15 5.260819020617639 1103 16 5.103984267091796 1103 17 2.9164270079485384 1103 18 1.8540876905321255 1103 19 3.8733828219943867 1103 20 4.152382332088951 1103 21 6.078433205166135 1103 22 2.284516744241909 1103 23 4.3048678188687255 1103 24 5.265794975655286 1103 25 1.629178400371611 1104 1 5.243855581432058 1104 2 9.754996157969886 1104 3 6.569357251388837 1104 4 7.181161026385951 1104 5 7.104127659464002 1104 6 6.827690527836232 1104 7 6.954966066211021 1104 8 6.775268957619758 1104 9 5.774532257693796 1104 10 4.584521212195902 1104 11 7.704753640446679 1104 12 4.698951377892307 1104 13 8.871954426896533 1104 14 6.623831307433562 1104 15 6.0201333265169765 1104 16 5.568942071389131 1104 17 5.839072935289451 1104 18 3.1928347743859575 1104 19 4.292884327115914 1104 20 3.486785097637231 1104 21 2.6024084064563127 1104 22 4.864810326534034 1104 23 5.201657893211699 1104 24 6.091923645326766 1104 25 5.0778332221190965 1105 1 2.856418901846247 1105 2 8.804602737446677 1105 3 5.848982435804707 1105 4 5.3237183629565195 1105 5 1.8165317546139939 1105 6 4.590523121213426 1105 7 5.62166855569631 1105 8 2.6182601228529414 1105 9 3.5680466653448626 1105 10 3.1353279993877265 1105 11 6.277782325067168 1105 12 3.786925055858351 1105 13 3.863778226774791 1105 14 3.2423844897035003 1105 15 3.8311427390342168 1105 16 4.002422536203099 1105 17 4.667678802895189 1105 18 2.3443635776516203 1105 19 2.1256479343419237 1105 20 1.4412227710913186 1105 21 1.7366880926828117 1105 22 3.4615044248822855 1105 23 0.8350976059872046 1105 24 3.835728303783416 1105 25 4.43750865018745 1106 1 6.2610793196415635 1106 2 10.473013057283948 1106 3 7.645336181890645 1106 4 8.88042414469578 1106 5 8.56786142857555 1106 6 7.690538283414909 1106 7 7.629276062519299 1106 8 7.41079520886862 1106 9 6.288147315104823 1106 10 5.602631308154887 1106 11 8.122081848394814 1106 12 5.41200725543381 1106 13 3.990931174204544 1106 14 5.521599837258638 1106 15 5.774799827959676 1106 16 5.777615641319745 1106 17 6.296983420041084 1106 18 6.43975597201878 1106 19 6.366094895598238 1106 20 5.588027805623657 1106 21 6.216073461328744 1106 22 6.324657890211952 1106 23 6.655152939504529 1106 24 7.272637361239639 1106 25 6.578368909205551 1107 1 3.7932523635874595 1107 2 -13.08377364631716 1107 3 -5.206520179661007 1107 4 1.4141039920875365 1107 5 4.0129417406918755 1107 6 4.455421138325466 1107 7 4.478892512551262 1107 8 4.9382931034025255 1107 9 3.35929938556437 1107 10 2.8251486338578076 1107 11 5.264035923866892 1107 12 2.1461629888970215 1107 13 3.900725903943872 1107 14 3.538060523386914 1107 15 3.496119828365547 1107 16 3.1541871307721188 1107 17 3.953122756235821 1107 18 -1.3822661886460423 1107 19 1.4596841318467533 1107 20 1.7526524238493835 1107 21 -1.9266771398706126 1107 22 1.0564113629626561 1107 23 -1.7079055163517118 1107 24 1.2886769541490395 1107 25 2.199950697684848 1108 1 5.102925248841739 1108 2 9.83483100484563 1108 3 0.6939398254501619 1108 4 5.952501640950302 1108 5 6.935081616320122 1108 6 6.867125712820737 1108 7 7.082201993767718 1108 8 6.647126905259583 1108 9 5.195465157776656 1108 10 4.375898271816748 1108 11 6.56150589575547 1108 12 1.936498622255911 1108 13 2.864649577169871 1108 14 4.469336611639747 1108 15 4.405077150113566 1108 16 4.595302217236043 1108 17 -0.11698543015143414 1108 18 3.380554324174472 1108 19 4.195649716649548 1108 20 4.658883789383076 1108 21 4.1007953876019165 1108 22 1.4140653368956997 1108 23 4.114992515848645 1108 24 4.734775429317281 1108 25 5.136295360773694 1109 1 3.5716503371249892 1109 2 -13.08377364631716 1109 3 -5.154578858229792 1109 4 2.186140184961072 1109 5 1.479719108335459 1109 6 2.4791072693263203 1109 7 3.529995644260744 1109 8 3.8301921892380904 1109 9 3.4622768540375084 1109 10 2.598298887518169 1109 11 0.5452869154162485 1109 12 1.34552081746563 1109 13 2.2601197984676986 1109 14 3.1101034147272095 1109 15 3.3635086955138007 1109 16 3.1131405129690073 1109 17 3.7178814175296147 1109 18 3.8669317580976212 1109 19 2.451136535714941 1109 20 1.4629199394585726 1109 21 0.3390422225763743 1109 22 2.7375238020154207 1109 23 3.2281646694778594 1109 24 4.419715710067486 1109 25 4.786714351099125 1110 1 2.188483675485436 1110 2 8.212522649979137 1110 3 5.435862129387967 1110 4 6.525316853300399 1110 5 7.4066468010636015 1110 6 3.364320880558541 1110 7 5.7217700054287555 1110 8 6.392030723806812 1110 9 4.398948008546327 1110 10 4.142710701419876 1110 11 2.3287489885799566 1110 12 4.91597034203771 1110 13 9.27299560954691 1110 14 6.721108956435348 1110 15 6.188597104176157 1110 16 5.564206124124446 1110 17 5.889562859342593 1110 18 6.134115577735724 1110 19 5.141341980171269 1110 20 5.311769232966192 1110 21 3.2233025101090362 1110 22 5.056614863166157 1110 23 5.660090684977824 1110 24 6.663219078691164 1110 25 7.0862280006288145 1111 1 4.864804407049615 1111 2 9.088510997877597 1111 3 7.160984038966806 1111 4 7.982517788029423 1111 5 8.090914329900418 1111 6 7.225413318430004 1111 7 7.063450276884307 1111 8 7.256881818058655 1111 9 5.675634735607588 1111 10 5.080488799310334 1111 11 6.793720658607807 1111 12 6.333202541105113 1111 13 7.812566871964613 1111 14 5.9851218889876066 1111 15 5.6379686162817535 1111 16 5.376529395249218 1111 17 6.509991917037567 1111 18 6.397696358170439 1111 19 5.496869792627919 1111 20 5.013168125376321 1111 21 7.575442463372585 1111 22 6.798323944762878 1111 23 6.830339946051055 1111 24 6.531196606781052 1111 25 6.95888184144745 1112 1 -1.885540320238782 1112 2 7.143455500375074 1112 3 5.30008060210756 1112 4 -0.2693719279655653 1112 5 3.470694278981327 1112 6 2.5607755154919225 1112 7 5.250428024019659 1112 8 5.38425765339392 1112 9 4.1026863118372034 1112 10 2.8013575479861856 1112 11 -0.2999437206496651 1112 12 3.611972906049221 1112 13 7.506129448944733 1112 14 4.809018312801079 1112 15 2.989537484534882 1112 16 3.3618659173099665 1112 17 4.352254943958613 1112 18 4.578983411865305 1112 19 4.1079743204054004 1112 20 3.9518497589527577 1112 21 -1.7904945305336426 1112 22 2.151440494904538 1112 23 3.7573574086427577 1112 24 5.342030200455305 1112 25 5.160057146046327 1113 1 -17.390813570478475 1113 2 -13.08377364631716 1113 3 -7.118702115372354 1113 4 -5.741584437130303 1113 5 -1.3836655735624994 1113 6 -0.4980900563622015 1113 7 -19.617261333602585 1113 8 -9.081195251379947 1113 9 -4.844272532713429 1113 10 -4.109235609349966 1113 11 -5.534965075134107 1113 12 -3.2327120576250827 1113 13 -1.9540937861699348 1113 14 -2.399173810906241 1113 15 -2.408308096650611 1113 16 -2.7648653853392204 1113 17 -2.528045095520266 1113 18 -3.2008936396264325 1113 19 -2.715844261328698 1113 20 -3.6138702691438986 1113 21 -0.9521046535334214 1113 22 -1.8845006661846675 1113 23 -2.228144101296177 1113 24 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2204 6 3.5271033803678193 2204 7 4.433523523276456 2204 8 4.180878311361695 2204 9 2.905875846960779 2204 10 2.497368150260151 2204 11 4.021435495670897 2204 12 4.272579739274992 2204 13 2.4775424392091594 2204 14 3.5834650265476498 2204 15 3.7378653865931 2204 16 3.455362640396384 2204 17 -1.2422594239109017 2204 18 2.131353386607884 2204 19 2.7806839999380344 2204 20 2.912127528615347 2204 21 4.6103486229169235 2204 22 4.105821325114999 2204 23 3.1269904279625145 2204 24 4.516404674259032 2204 25 4.717796425364369 2205 1 5.726994648699609 2205 2 9.85229941304357 2205 3 6.998788666743728 2205 4 7.622315643449873 2205 5 7.72010216018106 2205 6 7.275015699015917 2205 7 7.362647342196503 2205 8 6.9255930180997005 2205 9 6.068905989006591 2205 10 5.486027252955605 2205 11 7.509617739311722 2205 12 6.781807025225991 2205 13 9.540697804189744 2205 14 7.422939343197243 2205 15 6.429510866551109 2205 16 5.959613895787925 2205 17 6.878723639370953 2205 18 6.678236094458981 2205 19 5.08871218142275 2205 20 5.433105532384683 2205 21 -0.4824150093071111 2205 22 3.8462101486518527 2205 23 5.201016155583659 2205 24 7.049520448554176 2205 25 7.547035348194154 2206 1 3.869094263622909 2206 2 8.157701008060721 2206 3 5.819073581416625 2206 4 1.5892369236720825 2206 5 5.064743702171256 2206 6 5.741383562997566 2206 7 5.939420694047186 2206 8 5.879720493217011 2206 9 4.762637445354045 2206 10 3.814508199023823 2206 11 3.117066763994508 2206 12 4.305852224392709 2206 13 7.3046015981421455 2206 14 0.10469764970146045 2206 15 1.882773497277281 2206 16 2.962298911464547 2206 17 3.9234051620020978 2206 18 -1.0430188768180848 2206 19 0.4349529490510555 2206 20 1.9647344278668726 2206 21 2.9964352316288547 2206 22 -1.8175694948891703 2206 23 -2.87100370848216 2206 24 0.8180577883685842 2206 25 2.3595808524762916 2207 1 1.1528667269192958 2207 2 -13.08377364631716 2207 3 -6.039856917272838 2207 4 -4.273587498577932 2207 5 0.8430376051743558 2207 6 1.2174110071926556 2207 7 2.385526770486635 2207 8 2.819490592406705 2207 9 0.18045561471316418 2207 10 0.19082601719318504 2207 11 -1.687820769625489 2207 12 -2.846242065775691 2207 13 -0.31684153481315747 2207 14 -5.461033938532825 2207 15 -1.7284808497667292 2207 16 -0.4973236880249452 2207 17 0.5889568725532115 2207 18 0.4702740839685795 2207 19 0.06638443278497164 2207 20 -0.30885267575980535 2207 21 -3.7582981211198465 2207 22 -4.695222690716008 2207 23 -1.7457951360257162 2207 24 -0.09235712948727581 2207 25 0.4360340071882298 2208 1 4.868708130141542 2208 2 5.95077019779267 2208 3 6.038265150155322 2208 4 8.014545401873498 2208 5 3.4152459614194797 2208 6 6.073039569495712 2208 7 6.748549118124608 2208 8 6.693373128190256 2208 9 5.141027031995296 2208 10 4.919183842817727 2208 11 6.482443733948752 2208 12 5.981450302705152 2208 13 8.69655157222304 2208 14 3.1426856738490905 2208 15 4.685767868545032 2208 16 5.064392440667422 2208 17 5.986402880304254 2208 18 0.7908220900666834 2208 19 3.423980390651426 2208 20 4.5833455718587395 2208 21 7.16729399085871 2208 22 5.953281100842137 2208 23 1.2537010790015621 2208 24 3.0352297075662733 2208 25 4.3916054409339225 2209 1 3.1786713663534023 2209 2 4.482795838619671 2209 3 4.817700497620894 2209 4 1.1275860882226958 2209 5 4.829118760950529 2209 6 5.328524225714265 2209 7 5.634439346669524 2209 8 4.0413431146158025 2209 9 4.0440381162214685 2209 10 3.3383616113565004 2209 11 -0.971293372686894 2209 12 2.759734791244334 2209 13 1.9073791146787151 2209 14 3.4488589086750956 2209 15 3.812614558125585 2209 16 3.3011371195588453 2209 17 0.9947240121227914 2209 18 3.0684140834628018 2209 19 3.7983624603210018 2209 20 3.807055503762669 2209 21 5.8801181057001415 2209 22 4.454445301812921 2209 23 -0.09629603739529458 2209 24 -3.7566059941951337 2209 25 -1.1763481735119825 2210 1 -2.8683526329784748 2210 2 -13.08377364631716 2210 3 -7.601148012765403 2210 4 -2.6081745106597154 2210 5 -1.5912785832550114 2210 6 -1.8518741054932164 2210 7 -1.7715582086025847 2210 8 -19.69723803212861 2210 9 -12.692558587192039 2210 10 -7.391655395446222 2210 11 -9.28610143877047 2210 12 -4.9065892718630515 2210 13 -5.446379464645872 2210 14 -4.33533691251052 2210 15 -5.807687692238847 2210 16 -5.370577492625316 2210 17 -4.7647293786085 2210 18 -4.9499745219793745 2210 19 -9.962981418748484 2210 20 -7.194137439932668 2210 21 -5.257978650859624 2210 22 -9.389362589647234 2210 23 -7.4352192182747885 2210 24 -10.047718421308836 2210 25 -7.967667491417718 2211 1 -0.15125094622400326 2211 2 4.167717407360771 2211 3 0.3471795996848961 2211 4 1.142967077312452 2211 5 0.5251032041455836 2211 6 -0.4276868074067366 2211 7 -19.617261333602585 2211 8 -12.991472624573937 2211 9 -9.313879489900787 2211 10 -7.893519212543637 2211 11 -9.387865852289355 2211 12 -5.3878326708441655 2211 13 -6.783221692528576 2211 14 -5.516624188852468 2211 15 -5.851273831652367 2211 16 -11.386581080473228 2211 17 -8.088903474657208 2211 18 -6.445718806273611 2211 19 -8.811214287162997 2211 20 -7.326959647313384 2211 21 -5.017189013682685 2211 22 -6.282008666312244 2211 23 -7.935922032638606 2211 24 -20.336780921308836 2211 25 -20.114151866417718 2212 1 -0.32364939137399773 2212 2 -13.08377364631716 2212 3 -7.074406025158794 2212 4 -18.588643260659715 2212 5 -18.87350514575501 2212 6 -19.725897542993216 2212 7 -9.132186706736913 2212 8 -8.53092673148895 2212 9 -4.450210756035322 2212 10 -4.594880342141316 2212 11 -4.820349839623347 2212 12 -2.1323209649600656 2212 13 -5.2895589355947 2212 14 -3.04016100632715 2212 15 -2.873365164253773 2212 16 -3.156443780605059 2212 17 -3.413997685511486 2212 18 -2.225727453749098 2212 19 -2.7293705945214057 2212 20 -2.9345571327631568 2212 21 -0.8715680698361687 2212 22 -2.072504497296489 2212 23 -2.227259296899522 2212 24 -1.2898726057438061 2212 25 -1.8369663653516213 2213 1 -17.390813570478475 2213 2 -13.08377364631716 2213 3 -8.899481403459236 2213 4 -6.43335691704738 2213 5 -18.87350514575501 2213 6 -10.957175076032865 2213 7 -19.617261333602585 2213 8 -10.859132305256363 2213 9 -8.873644249361643 2213 10 -8.120454954917587 2213 11 -7.330980293396021 2213 12 -6.150665366301379 2213 13 -17.842863839645872 2213 14 -12.22603074510964 2213 15 -7.906092496754265 2213 16 -7.141842463715623 2213 17 -5.902261213685591 2213 18 -5.8191237510542635 2213 19 -6.760789960874035 2213 20 -6.626823460031787 2213 21 -4.599560423987377 2213 22 -5.524227161508467 2213 23 -5.2859299148606915 2213 24 -4.563653168004873 2213 25 -5.3156937166379805 2214 1 -17.390813570478475 2214 2 -13.08377364631716 2214 3 -11.827810360801564 2214 4 -18.588643260659715 2214 5 -18.87350514575501 2214 6 -19.725897542993216 2214 7 -15.86040347824099 2214 8 -14.289507358811901 2214 9 -13.300957024692039 2214 10 -13.272110507665673 2214 11 -13.537504181912613 2214 12 -11.72198486491168 2214 13 -17.842863839645872 2214 14 -15.75132818433097 2214 15 -22.149484567238847 2214 16 -17.1164983350505 2214 17 -21.8692215661085 2214 18 -16.616472339934486 2214 19 -13.947987166098859 2214 20 -12.854115911721944 2214 21 -10.509985352854637 2214 22 -11.468361868793119 2214 23 -11.532958269085261 2214 24 -10.380421819064447 2214 25 -10.099189272901508 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 130220.0 3 2.0 4 691.0 5 2503.0 6 3155.0 7 3083.0 8 2657.0 9 2611.0 10 2500.0 11 2304.0 12 2127.0 13 2339.0 14 3251.0 15 6224.0 16 8504.0 17 9298.0 18 9556.0 19 11485.0 20 13420.0 21 15429.0 22 16786.0 23 18080.0 24 18735.0 25 18808.0 26 16248.0 27 13703.0 28 14646.0 29 16389.0 30 16129.0 31 18289.0 32 22744.0 33 27724.0 34 32830.0 35 36400.0 36 34753.0 37 34676.0 38 21336.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.472448699587645 17.315463235487595 11.9328198593397 26.279268205585065 2 14.731651095426542 19.68146954428624 39.61333686916079 25.973542491126434 3 19.32961533259112 23.42531223229181 28.771325612932536 28.47374682218453 4 11.839804491763836 15.684420911045645 37.007322086557856 35.46845251063265 5 13.677967218670858 36.80358504598989 35.51473841779719 14.003709317542059 6 33.848994861883206 35.66101824653393 17.277467874809833 13.212519016773033 7 28.894910215288476 30.435880691145645 21.897944530835645 18.771264562730234 8 26.03295696204323 36.10333638818022 19.496233338405087 18.36747331137146 9 26.450587329191734 15.160696485549552 19.03150212745692 39.3572140578018 10 14.529195125440397 29.192962961730785 33.90334053050592 22.3745013823229 11 34.53823271787489 21.838049728117113 23.51114849922381 20.112569054784185 12 24.035415227712818 24.181209886896053 30.5862580704651 21.19711681492603 13 28.60574026933017 20.422261518431434 24.879617574473873 26.092380637764524 14 20.986848133001274 21.20973854521527 27.088847983074988 30.714565338708468 15 23.41427766132326 29.998852332297055 22.857321276071467 23.729548730308217 16 22.88244119018496 27.497685451117405 26.439412881731624 23.180460476966015 17 22.009218238074833 27.37381440029153 28.00596553620655 22.611001825427085 18 22.04716938733727 26.9282305570675 29.06448546950502 21.960114586090207 19 23.503479390661624 26.425568655373134 26.565143542540397 23.505808411424844 20 23.700096249557827 27.007296868187463 27.00400628501386 22.288600597240844 21 22.921027902207904 25.40006401708948 26.21379996200055 25.465108118702062 22 23.132306482357805 26.949339500659832 27.209940148495715 22.708413868486648 23 22.67895470646485 26.735354444369637 27.406686055581925 23.179004793583594 24 23.02780242638051 26.6438422601192 27.255646525864268 23.07270878763602 25 23.058946410711616 26.494106672754057 27.37502648178956 23.071920434744765 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 554.0 1 554.0 2 1113.0 3 1672.0 4 1672.0 5 1672.0 6 2526.5 7 3381.0 8 3381.0 9 3381.0 10 3814.5 11 4248.0 12 4248.0 13 4248.0 14 4996.0 15 5744.0 16 5744.0 17 5744.0 18 7971.0 19 10198.0 20 10198.0 21 10198.0 22 14455.0 23 18712.0 24 18712.0 25 18712.0 26 24867.0 27 31022.0 28 31022.0 29 31022.0 30 38671.0 31 46320.0 32 46320.0 33 46320.0 34 52657.0 35 58994.0 36 58994.0 37 58994.0 38 63909.0 39 68824.0 40 68824.0 41 68824.0 42 71725.5 43 74627.0 44 74627.0 45 74627.0 46 75655.5 47 76684.0 48 76684.0 49 76684.0 50 72943.0 51 69202.0 52 69202.0 53 69202.0 54 61852.0 55 54502.0 56 54502.0 57 54502.0 58 46457.5 59 38413.0 60 38413.0 61 38413.0 62 31184.5 63 23956.0 64 23956.0 65 23956.0 66 18421.0 67 12886.0 68 12886.0 69 12886.0 70 9421.5 71 5957.0 72 5957.0 73 5957.0 74 4237.0 75 2517.0 76 2517.0 77 2517.0 78 1686.5 79 856.0 80 856.0 81 856.0 82 558.5 83 261.0 84 261.0 85 261.0 86 176.0 87 91.0 88 91.0 89 91.0 90 51.0 91 11.0 92 11.0 93 11.0 94 6.0 95 1.0 96 1.0 97 1.0 98 1.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 12.724499085518385 2 35.670360133522514 3 5.4099584177417634 4 13.414584136409491 5 12.883118587351447 6 14.282316468050555 7 9.072477794089906 8 7.285178836516932 9 0.11351054319387831 10 1.3898480238175301 11 5.429478294389265 12 5.717191434218836 13 15.587195617049545 14 1.5320642679636176 15 2.8097140092022275 16 2.9078054901703476 17 2.7716584513684417 18 1.4539847613736088 19 1.3982136852378881 20 0.30181994144037005 21 4.169051973721982 22 1.5555209264559942 23 0.11416667350135735 24 8.680768000524905 25 0.11908765080745037 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 609635.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 95.02142999029567 #Duplication Level Percentage of deduplicated Percentage of total 1 97.44314355101473 92.59186842967078 2 1.9231633161386505 3.6548345680874728 3 0.3328525189752726 0.9488436698670728 4 0.10664691722950287 0.40534970316816255 5 0.055183363352084465 0.2621801048694571 6 0.02966062648961866 0.16910370860709653 7 0.025956843152597962 0.1726519448075571 8 0.014692367306870768 0.11168718011532236 9 0.011200629362711748 0.09578698369525479 >10 0.05084362575471519 0.8616691658407274 >50 0.0043791203847766075 0.2969357201838173 >100 0.0022771208384714105 0.42908882108728763 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 1.6403257686976633E-4 0.0 0.0 0.0 6 0.0 1.6403257686976633E-4 0.0 0.0 0.0 7 0.0 1.6403257686976633E-4 0.0 0.0 0.0 8 0.0 1.6403257686976633E-4 0.0 0.0 0.0 9 0.0 1.6403257686976633E-4 0.0 1.6403257686976633E-4 0.0 10 0.0 1.6403257686976633E-4 0.0 1.6403257686976633E-4 0.0 11 0.0 1.6403257686976633E-4 0.0 1.6403257686976633E-4 0.0 12 0.0 1.6403257686976633E-4 0.0 1.6403257686976633E-4 0.0 13 0.0 1.6403257686976633E-4 0.0 1.6403257686976633E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCCTAA 135 1.8189894E-12 16.701504 1 GAACAGG 65 4.7206588E-5 16.323643 1 TCCTACA 235 0.0 16.175858 2 TCCAACG 40 6.15085E-4 14.960702 18 CTAGGAC 110 3.5652192E-10 14.834582 3 TAAGTAC 45 0.004685634 14.504925 3 GTTCTTA 55 0.00476186 14.468682 1 TCCTAAA 170 3.6379788E-12 14.374764 2 TAGACTG 80 7.439594E-7 14.255505 5 TTGGACG 45 0.005266416 14.251884 4 CTTATAC 55 0.0011049223 13.84561 3 CTGTAGG 205 0.0 13.586446 1 TCCTATA 80 2.2687837E-4 13.576166 2 GTCCTAC 245 0.0 13.533632 1 CACTATT 45 0.0074434015 13.526871 7 TAGGACC 135 5.0931703E-11 13.460113 4 GTACAGG 80 2.7648234E-4 13.2629595 1 ACTACAC 50 0.009412997 13.054432 3 TAATAGT 75 6.8822395E-5 12.826695 4 CTACACT 75 6.8822395E-5 12.826695 4 >>END_MODULE