FastQCFastQC Report
Thu 26 May 2016
SRR1926481_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926481_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences609635
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC9870.16190015337045938No Hit
GTACATGGGAAGCAGTGGTATCAAC8820.1446767327991339No Hit
GTATCAACGCAGAGTACTTTTTTTT8290.13598300622503628No Hit
CTGTAGGACGTGGAATATGGCAAGA6410.10514488177352022No Hit
GTCCTACAGTGGACATTTCTAAATT6340.10399665373543186No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGAAC456.5551914E-414.8360943
GCATAAG400.00514096414.3050531
CAAGACG400.00514378214.3038714
ACCTAGG500.001454809713.3513831
CTATAGG751.4177205E-512.7156021
CCTAATA550.002970119212.1416312
TCATATA550.002970119212.1416312
TAATACT550.002979322812.136624
GTCCTAG802.7489454E-511.9208771
TAGGACC2000.011.9198934
GTAGGAC3700.011.857423
TATTTAA657.734529E-411.7413582
ATGTTAT657.7560043E-411.7374791
TAGAGTG657.761381E-411.7365115
TCCTAGG751.9908894E-411.4478242
TAATACA855.1099552E-511.2187224
AGGACCT3150.011.2009155
CTAGTCC600.005699076711.1270713
TCTATAC600.005699076711.1270713
TGTAGGA4150.011.0340462
Produced by FastQC (version 0.11.5)