FastQCFastQC Report
Thu 26 May 2016
SRR1926480_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926480_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences714707
Sequences flagged as poor quality0
Sequence length25
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT8280.1158516706846302No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCAGT450.001239327517.703311
GTCCTAA3450.017.3184551
TAGGACC2200.016.5168674
CTGTAGG5350.016.3796981
TGTAGGA5400.016.1243212
TCTTACA707.1603674E-515.5484512
GTTTAGA604.7192746E-415.4903961
GTTCAGG708.815807E-515.1742661
TCCTAAA4050.015.11654952
GTCCTAC6750.014.949461
TCCTACA6850.014.895872
GTATATA1005.654092E-714.6052291
GTAGGAC5750.014.1858643
GTCTATA600.00841596213.2774821
TCTATAC500.00943243613.0509963
TTTAGAA1706.166374E-1012.8046072
CCTACAG7700.012.7120093
TCTGGGG750.002120215212.6979032
ATAGGAC704.7888354E-412.429523
GTATTAA750.00254587912.3923171
Produced by FastQC (version 0.11.5)