FastQCFastQC Report
Thu 26 May 2016
SRR1926480_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926480_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences714707
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA18580.25996667165705667No Hit
GTCCTACAGTGGACATTTCTAAATT18450.2581477444603173No Hit
GTCCTAAAGTGTGTATTTCTCATTT13770.19266636537770024No Hit
CTTTAGGACGTGAAATATGGCGAGG12930.1809132973372305No Hit
CCCATGTACTCTGCGTTGATACCAC12860.17993387500052468No Hit
GTCCTACAGTGTGCATTTCTCATTT12530.17531659827034016No Hit
GTACATGGGAAGCAGTGGTATCAAC11530.16132485060311427No Hit
CTGTAGGACCTGGAATATGGCGAGA10510.1470532679825439No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTATAG508.4134845E-515.2604721
GGACCTA500.001451668413.3557366
CCGTGAT500.001529757613.26321417
TCTTAGA655.2072206E-513.2164192
TGTAGGA10800.012.6385412
GATATAC2000.012.3991341
GTAGGAC9850.012.3043973
TCTACAC1251.3078534E-912.2152613
CTGTAGG10550.012.2047611
TAGGACC3950.012.0774144
TAGGACA3100.012.0033894
CTAGGAC1750.011.9971313
TCTAGGA2000.011.931492
CCTCTAG657.751439E-411.7388251
ATACACT2450.011.6830474
GGCGAGG3500.011.63751919
TATACTG1406.311893E-1011.5848735
TAGGACT1501.6189006E-1011.4493884
GTCTTGA751.9958084E-411.4453541
GACGTGG4900.011.4335397
Produced by FastQC (version 0.11.5)