FastQCFastQC Report
Thu 26 May 2016
SRR1926479_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926479_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1000994
Sequences flagged as poor quality0
Sequence length25
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT13320.13306773067570835No Hit
CTGTAGGACGTGGAATATGGCAAGA10780.10769295320451472No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAAG604.6594272E-415.5181421
GTCCTAC9150.015.1183771
GTCCTAA2850.014.9347521
TCCTAAA2500.014.7143012
ATGGGCG507.573551E-414.5567855
TACAGCG507.573551E-414.5567855
GATATAC2500.014.3653661
TAGGACT1405.456968E-1213.7749744
TCCTACA10050.013.6921362
TGTAGGA8250.013.5417812
TAGGACC3800.013.5333094
ACTGTTC2600.013.4902958
ATAGGAC1302.910383E-1013.4296193
GGACTGA1253.3833203E-1013.3054346
GTCTAGA1101.6261565E-613.3012651
GTCTTAG904.9004528E-513.3012651
GTCTTAC904.9004528E-513.3012651
GATTAAA1851.8189894E-1112.94177151
CCTGGAC1355.311449E-1012.9322243
CTGGACT1253.0631782E-912.8566444
Produced by FastQC (version 0.11.5)