##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926479_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1000994 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.425491061884486 27.0 2.0 30.0 2.0 31.0 2 16.0396126250507 16.0 2.0 30.0 2.0 31.0 3 19.57552492822135 22.0 15.0 30.0 2.0 31.0 4 21.71878552718598 28.0 2.0 35.0 2.0 37.0 5 22.085377135127683 28.0 2.0 35.0 2.0 37.0 6 22.971652177735333 30.0 2.0 35.0 2.0 37.0 7 22.813869014199884 30.0 2.0 35.0 2.0 37.0 8 22.89643694167997 30.0 2.0 35.0 2.0 37.0 9 25.53477243619842 31.0 17.0 37.0 2.0 39.0 10 26.178569501915096 31.0 18.0 37.0 2.0 39.0 11 23.33835167843164 30.0 7.0 37.0 2.0 39.0 12 24.404457968779035 30.0 8.0 37.0 2.0 39.0 13 21.157916031464723 27.0 2.0 35.0 2.0 39.0 14 24.41081265222369 30.0 10.0 37.0 2.0 40.0 15 25.463660121838892 31.0 10.0 38.0 2.0 40.0 16 25.839890149191703 31.0 8.0 38.0 2.0 40.0 17 25.136416402096316 31.0 8.0 38.0 2.0 40.0 18 25.19476840021019 31.0 8.0 38.0 2.0 40.0 19 25.565092298255536 31.0 8.0 38.0 2.0 40.0 20 25.799416380118164 31.0 2.0 38.0 2.0 40.0 21 23.682847249833664 30.0 2.0 37.0 2.0 40.0 22 24.589036497721263 30.0 2.0 38.0 2.0 40.0 23 24.572642793063693 31.0 2.0 38.0 2.0 40.0 24 23.701066140256586 31.0 2.0 38.0 2.0 40.0 25 23.482304589238296 30.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.2200895895520425 1101 2 9.89654870196952 1101 3 6.779697791006857 1101 4 8.178484090405263 1101 5 7.99710716666878 1101 6 7.668777782526199 1101 7 7.291082078123868 1101 8 5.5549531675716715 1101 9 5.664083699753267 1101 10 5.199532591810456 1101 11 7.9339881695026335 1101 12 5.36575415621672 1101 13 10.040817792494337 1101 14 7.716234168390006 1101 15 6.854096993714677 1101 16 6.06550087922194 1101 17 2.0754733289518654 1101 18 4.725363050208763 1101 19 5.201415838511586 1101 20 3.186520407471349 1101 21 0.6566635113602928 1101 22 4.2379382538856625 1101 23 5.337950934281949 1101 24 6.734270876324427 1101 25 7.394819702431931 1102 1 5.4302193161140195 1102 2 10.36406112188934 1102 3 6.911112529755677 1102 4 7.930862188725744 1102 5 7.769734062954122 1102 6 6.786098409582312 1102 7 6.715134101938148 1102 8 6.910795524492862 1102 9 6.056705668027533 1102 10 5.438760891913013 1102 11 7.935565133048669 1102 12 5.182378868194451 1102 13 9.575791354380833 1102 14 7.444207956698634 1102 15 5.145821264835202 1102 16 5.617707829049458 1102 17 6.341159720981803 1102 18 6.285501434087507 1102 19 5.014305251279129 1102 20 5.49189260549748 1102 21 4.387827187922937 1102 22 5.416197067700075 1102 23 6.007176171026266 1102 24 7.452632379234618 1102 25 6.98128565253915 1103 1 4.412857511949223 1103 2 8.760019567692728 1103 3 6.09341448757187 1103 4 7.670145774014326 1103 5 6.983380503988556 1103 6 6.52306985614257 1103 7 6.720691406407703 1103 8 6.198299124650934 1103 9 5.293095061124607 1103 10 4.4258364918835404 1103 11 4.783253999015621 1103 12 3.8280556644221555 1103 13 7.892000438165724 1103 14 6.22279187219706 1103 15 5.208727915248129 1103 16 5.034084362047 1103 17 3.01468667025809 1103 18 1.9177655483816416 1103 19 3.8337226619784666 1103 20 4.169787300036624 1103 21 6.369127799447462 1103 22 1.9705041963479566 1103 23 4.323121354852013 1103 24 5.627620155442095 1103 25 1.5647785091557367 1104 1 5.0965099789010075 1104 2 9.811753506868842 1104 3 6.678700158049235 1104 4 7.521126916168651 1104 5 7.172105035674949 1104 6 6.760846879181521 1104 7 6.94396777161483 1104 8 6.8821721878390605 1104 9 5.768449614343034 1104 10 4.710623595082353 1104 11 7.608774906197034 1104 12 4.623987988530455 1104 13 8.991240057117874 1104 14 6.4681176390904795 1104 15 5.88731722045242 1104 16 5.529423153680831 1104 17 6.004799738194475 1104 18 3.534083404806136 1104 19 4.23977405529595 1104 20 3.442899619462615 1104 21 2.563604650032257 1104 22 4.514982860504201 1104 23 5.029537770641294 1104 24 6.063396644301818 1104 25 5.054866846572352 1105 1 3.5774713094569606 1105 2 9.41665550409254 1105 3 6.45600106996228 1105 4 5.783335772213086 1105 5 2.3698368075140763 1105 6 5.2091102366957145 1105 7 6.0528042623575296 1105 8 2.977632337901518 1105 9 4.042285321322087 1105 10 3.880969210611539 1105 11 7.037345272820662 1105 12 4.386460092200572 1105 13 4.878004793894487 1105 14 3.7539893701092595 1105 15 4.5137124409485665 1105 16 4.823504526123703 1105 17 5.525842903371451 1105 18 2.965509903127913 1105 19 3.111768806503509 1105 20 1.9351723562844754 1105 21 2.756715609956885 1105 22 4.396381482696185 1105 23 1.6796721416669271 1105 24 4.538103379336377 1105 25 5.244920643462834 1106 1 6.539423388082792 1106 2 11.26476796501928 1106 3 7.944876795096718 1106 4 9.331917828848017 1106 5 8.89554100202766 1106 6 8.060678501643874 1106 7 7.939494591934416 1106 8 7.66110540342676 1106 9 6.538653876722119 1106 10 6.105562713343282 1106 11 8.615665206195079 1106 12 5.893045738081774 1106 13 4.983634369029005 1106 14 6.1980527026304735 1106 15 6.3564607780956734 1106 16 6.25311596712487 1106 17 6.765370791687612 1106 18 6.714716813462044 1106 19 6.418237570984303 1106 20 5.836491332632715 1106 21 6.06734560707466 1106 22 6.457712051641085 1106 23 7.005772841203701 1106 24 7.683608939164845 1106 25 6.55850038317082 1107 1 3.89390416290318 1107 2 -13.383911068145421 1107 3 -5.2842929926445965 1107 4 1.911901208725375 1107 5 4.389359673672679 1107 6 4.8206681812903724 1107 7 4.683019082286933 1107 8 5.399041089442171 1107 9 3.6373733077966257 1107 10 3.067636694656528 1107 11 5.771136338015186 1107 12 3.181898475856446 1107 13 4.497381822552203 1107 14 3.9414325031633943 1107 15 3.614337090067412 1107 16 3.442585685338244 1107 17 3.855118813726559 1107 18 -1.0558272202596477 1107 19 1.619055196509123 1107 20 2.106177150853643 1107 21 -1.1746013801119695 1107 22 1.9302261076808591 1107 23 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5.80579243812269 2205 21 -0.1924361054979471 2205 22 4.347363541241982 2205 23 5.7601348423279255 2205 24 7.27040114452366 2205 25 7.767501694769095 2206 1 4.51775712068898 2206 2 8.872913444389399 2206 3 6.280246889716555 2206 4 2.4625170043104276 2206 5 5.9620705067257695 2206 6 6.353407821267275 2206 7 6.514907839337969 2206 8 6.366687551076296 2206 9 5.16712375354253 2206 10 4.226952308136138 2206 11 3.445818605259685 2206 12 4.80876315311103 2206 13 8.329395578810093 2206 14 0.9405257830283702 2206 15 2.8344238915471003 2206 16 3.736212946586587 2206 17 4.902428792724155 2206 18 -0.7914669052870273 2206 19 1.559689139187153 2206 20 3.093047158023648 2206 21 3.8761076889929242 2206 22 -1.0931842767710158 2206 23 -2.3115736070685458 2206 24 1.7388845861825892 2206 25 3.2574057492413964 2207 1 1.3155630308120152 2207 2 -13.383911068145421 2207 3 -6.068590201334679 2207 4 -4.216465782062951 2207 5 1.0665716643115708 2207 6 1.6844064912750767 2207 7 2.934270110462517 2207 8 2.911835621952008 2207 9 0.49862645404640205 2207 10 0.2936241655330072 2207 11 -1.3313277749210464 2207 12 -2.4393320342875704 2207 13 0.3626034341857576 2207 14 -5.2825954242295445 2207 15 -1.343715295234972 2207 16 -0.4648723397589869 2207 17 0.7663513639788455 2207 18 0.7428683449088176 2207 19 0.10286589785156863 2207 20 0.08066345289102372 2207 21 -3.8471198223008862 2207 22 -4.24656876528017 2207 23 -1.4735997079668053 2207 24 0.2368136437313666 2207 25 0.8803089458307696 2208 1 4.78815443772497 2208 2 6.345221049046396 2208 3 6.223403591506276 2208 4 7.754622555169565 2208 5 3.743682718030744 2208 6 6.3030594393760495 2208 7 6.6232288157003545 2208 8 6.6068488615157435 2208 9 5.143515086878942 2208 10 4.598819681267386 2208 11 6.659258370223167 2208 12 6.257244977970025 2208 13 8.497220644136512 2208 14 2.871635323533827 2208 15 4.52749151535933 2208 16 5.109624132686417 2208 17 5.969232502433115 2208 18 0.8013671294295186 2208 19 3.2694387507702167 2208 20 4.260120796331645 2208 21 6.785416299145503 2208 22 6.026749942568685 2208 23 1.6851154946220106 2208 24 2.846702968735933 2208 25 4.449602302839853 2209 1 2.9802469965953833 2209 2 4.419965784762217 2209 3 5.119594119923356 2209 4 0.3645704087056352 2209 5 4.938068385033372 2209 6 5.051049502994356 2209 7 5.6029898357615835 2209 8 4.250401338583085 2209 9 3.9920965177456402 2209 10 3.1687982440470535 2209 11 -0.5994906441122261 2209 12 2.9943207167221004 2209 13 1.8957981985284924 2209 14 3.5118540323462817 2209 15 3.846961251437584 2209 16 3.36361422277821 2209 17 1.1982927630204614 2209 18 3.2482827477146294 2209 19 3.857033212264845 2209 20 3.8284403539975145 2209 21 5.852223086190893 2209 22 4.599013104145822 2209 23 0.24326293050732772 2209 24 -3.013672084920149 2209 25 -0.04545408883663171 2210 1 -2.3412908180961622 2210 2 -13.383911068145421 2210 3 -7.499853960563657 2210 4 -2.247841589237016 2210 5 -0.9152923614907706 2210 6 -1.3323428385475111 2210 7 -1.16355128538531 2210 8 -20.243897407140974 2210 9 -12.746647927273314 2210 10 -7.01381061725327 2210 11 -9.84651823600583 2210 12 -4.50512752704666 2210 13 -5.178696376435516 2210 14 -3.82958510574381 2210 15 -5.519964126083419 2210 16 -4.873427329704622 2210 17 -4.7164299437554575 2210 18 -4.8068085131742215 2210 19 -10.435516649898148 2210 20 -7.238103080307436 2210 21 -5.353596275214201 2210 22 -9.738571224072256 2210 23 -7.5505891035750174 2210 24 -10.272075652082014 2210 25 -7.97368631902035 2211 1 0.07363422442023548 2211 2 4.504711686345596 2211 3 0.53664940502021 2211 4 1.3545457601691844 2211 5 0.9209216067541242 2211 6 -0.22375095310598425 2211 7 -20.13133171497958 2211 8 -13.089406389176903 2211 9 -9.17164828455398 2211 10 -7.445054096881126 2211 11 -9.62253327037623 2211 12 -5.122586404042071 2211 13 -6.872462543385627 2211 14 -4.839206674063021 2211 15 -5.283279976482859 2211 16 -11.7311781764598 2211 17 -7.900051483492213 2211 18 -6.111167337292095 2211 19 -8.749341267965114 2211 20 -6.7527837428487025 2211 21 -4.534388866642324 2211 22 -6.031299133408716 2211 23 -8.312100686614189 2211 24 -21.115154411031895 2211 25 -20.90805386078646 2212 1 -1.6241183461868687 2212 2 -13.383911068145421 2212 3 -7.962747037281503 2212 4 -19.078987173962553 2212 5 -19.32818018965306 2212 6 -20.223750953105984 2212 7 -10.182645093110308 2212 8 -9.542614572687706 2212 9 -5.686708329400144 2212 10 -6.166619396337193 2212 11 -5.515512248278704 2212 12 -3.3774647963106794 2212 13 -5.980670562289784 2212 14 -4.305114808923413 2212 15 -4.4144623277241735 2212 16 -4.482092840717112 2212 17 -4.574836671820506 2212 18 -3.793256305354749 2212 19 -4.176241285888949 2212 20 -4.453044187140325 2212 21 -2.362150691800803 2212 22 -3.6109361940461433 2212 23 -3.8497389104719097 2212 24 -2.7022038917893774 2212 25 -3.163399004341745 2213 1 -17.828760985160603 2213 2 -13.383911068145421 2213 3 -8.489379371845875 2213 4 -5.924489129633999 2213 5 -19.32818018965306 2213 6 -10.57707559456622 2213 7 -20.13133171497958 2213 8 -10.513780066854142 2213 9 -8.247580644085243 2213 10 -7.44504472839515 2213 11 -6.689000336244494 2213 12 -5.221793986270054 2213 13 -18.413779908655087 2213 14 -12.075093440295873 2213 15 -7.009829485027698 2213 16 -6.2792330445593265 2213 17 -4.950025329802202 2213 18 -5.054923631743609 2213 19 -5.816478180580564 2213 20 -5.706526843349135 2213 21 -3.7179112612273393 2213 22 -4.712006297958364 2213 23 -4.4524640277285705 2213 24 -3.5310605388024285 2213 25 -4.381978241490501 2214 1 -17.828760985160603 2214 2 -13.383911068145421 2214 3 -12.035364343590757 2214 4 -19.078987173962553 2214 5 -19.32818018965306 2214 6 -20.223750953105984 2214 7 -16.053946290486717 2214 8 -14.630073214804383 2214 9 -13.682154544760838 2214 10 -13.568397492734048 2214 11 -13.998542034215944 2214 12 -12.010002555960623 2214 13 -18.413779908655087 2214 14 -16.20141913869049 2214 15 -22.89705243157268 2214 16 -17.72618845642579 2214 17 -22.608434717025148 2214 18 -17.214814588244735 2214 19 -14.467829910514727 2214 20 -13.233753433819878 2214 21 -10.875841648531589 2214 22 -11.866818777816368 2214 23 -11.7914980151727 2214 24 -10.715454936952256 2214 25 -10.45576235064371 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 197081.0 3 6.0 4 1030.0 5 3809.0 6 4914.0 7 4651.0 8 4050.0 9 3979.0 10 3609.0 11 3439.0 12 3394.0 13 3489.0 14 5116.0 15 10069.0 16 14301.0 17 15486.0 18 16026.0 19 18976.0 20 22758.0 21 26281.0 22 27867.0 23 29792.0 24 31155.0 25 31772.0 26 26672.0 27 21884.0 28 23197.0 29 26851.0 30 26035.0 31 29709.0 32 37389.0 33 46427.0 34 55418.0 35 62552.0 36 60738.0 37 61278.0 38 39794.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.632447542833575 17.80845615823934 12.278786281799382 26.2803100171277 2 14.009715306596494 19.463350907770064 41.280543508422944 25.246390277210494 3 19.50047604873219 23.05859727121689 28.68927212043294 28.75165455961798 4 11.529649782442007 15.860139311020166 37.78245586482766 34.82775504171016 5 13.288328136596439 37.08482591122886 36.07220898464875 13.554636967525946 6 33.30044389084437 36.47859037249043 17.40889507801329 12.812070658651905 7 28.725620418597536 30.229965632259674 21.971033376215058 19.07338057292773 8 24.979787196146773 37.923861687210504 19.69272220321181 17.40362891343091 9 26.138236588390605 15.308937072448694 19.47243669240309 39.08038964675761 10 14.23565346449394 29.433230167614745 34.6736096688735 21.657506699017816 11 34.125117194428725 21.50813392683689 24.597843622511466 19.76890525622292 12 24.280796642994112 23.95699762709747 31.592640084369865 20.16956564553855 13 28.944763131841846 20.81330023826554 24.9909999099991 25.250936719893513 14 19.994338324671716 22.234961555398176 27.459632660764182 30.31106745916593 15 22.44487391675319 32.050844495615614 22.75101000236438 22.753271585266816 16 21.610101095258607 27.910434104048026 28.550954251645255 21.928510549048113 17 21.15586585634042 28.3476825751768 29.122521081978736 21.37393048650404 18 20.992744029891597 27.207759164235508 31.177458038434946 20.622038767437946 19 23.32506932546851 26.50932968325581 26.827765850633885 23.337835140641797 20 23.382790506627128 27.652604265806424 27.986815873047675 20.97778935451877 21 21.91517158470173 26.01791908780103 26.504109629033383 25.562799698463856 22 22.353382886425326 28.595042999568737 27.63844846393871 21.413125650067226 23 21.605771086759322 28.01003319395008 28.231559555587115 22.15263616370348 24 22.829799306412074 26.66468769922032 28.63624314572576 21.86926984864185 25 22.331017624855225 27.315425098866015 28.392199536324885 21.961357739953872 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 795.0 1 795.0 2 1821.5 3 2848.0 4 2848.0 5 2848.0 6 4150.0 7 5452.0 8 5452.0 9 5452.0 10 6203.0 11 6954.0 12 6954.0 13 6954.0 14 8300.5 15 9647.0 16 9647.0 17 9647.0 18 13851.0 19 18055.0 20 18055.0 21 18055.0 22 26024.0 23 33993.0 24 33993.0 25 33993.0 26 45984.0 27 57975.0 28 57975.0 29 57975.0 30 72071.5 31 86168.0 32 86168.0 33 86168.0 34 97007.5 35 107847.0 36 107847.0 37 107847.0 38 114335.0 39 120823.0 40 120823.0 41 120823.0 42 123663.0 43 126503.0 44 126503.0 45 126503.0 46 125692.5 47 124882.0 48 124882.0 49 124882.0 50 115755.5 51 106629.0 52 106629.0 53 106629.0 54 92713.0 55 78797.0 56 78797.0 57 78797.0 58 65993.5 59 53190.0 60 53190.0 61 53190.0 62 42438.0 63 31686.0 64 31686.0 65 31686.0 66 24280.0 67 16874.0 68 16874.0 69 16874.0 70 12195.5 71 7517.0 72 7517.0 73 7517.0 74 5217.0 75 2917.0 76 2917.0 77 2917.0 78 1960.5 79 1004.0 80 1004.0 81 1004.0 82 661.5 83 319.0 84 319.0 85 319.0 86 205.0 87 91.0 88 91.0 89 91.0 90 56.0 91 21.0 92 21.0 93 21.0 94 12.5 95 4.0 96 4.0 97 4.0 98 3.5 99 3.0 100 3.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 12.801175631422366 2 35.91749800698106 3 5.355876258998555 4 13.48839253781741 5 13.044633634167639 6 14.343342717338963 7 9.22013518562549 8 7.452692024128018 9 0.11128937835791224 10 1.3895188182946152 11 5.379652625290461 12 5.652481433455145 13 15.640253587933595 14 1.5404687740386056 15 2.819597320263658 16 2.8318851062044326 17 2.786030685498614 18 1.4481605284347359 19 1.396911469998821 20 0.28211957314429453 21 4.186338779253422 22 1.5503589432104488 23 0.11118947765920675 24 8.797255528005163 25 0.11548520770354267 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1000994.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 92.13932199997667 #Duplication Level Percentage of deduplicated Percentage of total 1 96.5673984289701 88.97654618546918 2 2.5331207057264176 4.668000487394691 3 0.43440293803461055 1.200767765559207 4 0.16728670687899128 0.6165473500575636 5 0.08358533086824933 0.38507478576721077 6 0.049160023646839884 0.27177427489935885 7 0.028294496469070125 0.18249250046935991 8 0.022038285613650562 0.16244741555868822 9 0.01615630899663712 0.13397682212750409 >10 0.08530169377051902 1.432266769721811 >50 0.006630960571249323 0.41683764247928706 >100 0.005950481536350758 1.0078916108892628 >500 4.4909261152420145E-4 0.29606737994213284 >1k 2.2454630576210073E-4 0.24930900966475897 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1332 0.13306773067570835 No Hit CTGTAGGACGTGGAATATGGCAAGA 1078 0.10769295320451472 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 9.990069870548674E-5 0.0 12 0.0 1.9980139741097348E-4 0.0 9.990069870548674E-5 0.0 13 0.0 1.9980139741097348E-4 0.0 9.990069870548674E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTATAAG 60 4.6594272E-4 15.518142 1 GTCCTAC 915 0.0 15.118377 1 GTCCTAA 285 0.0 14.934752 1 TCCTAAA 250 0.0 14.714301 2 ATGGGCG 50 7.573551E-4 14.556785 5 TACAGCG 50 7.573551E-4 14.556785 5 GATATAC 250 0.0 14.365366 1 TAGGACT 140 5.456968E-12 13.774974 4 TCCTACA 1005 0.0 13.692136 2 TGTAGGA 825 0.0 13.541781 2 TAGGACC 380 0.0 13.533309 4 ACTGTTC 260 0.0 13.490295 8 ATAGGAC 130 2.910383E-10 13.429619 3 GGACTGA 125 3.3833203E-10 13.305434 6 GTCTAGA 110 1.6261565E-6 13.301265 1 GTCTTAG 90 4.9004528E-5 13.301265 1 GTCTTAC 90 4.9004528E-5 13.301265 1 GATTAAA 185 1.8189894E-11 12.9417715 1 CCTGGAC 135 5.311449E-10 12.932224 3 CTGGACT 125 3.0631782E-9 12.856644 4 >>END_MODULE