FastQCFastQC Report
Thu 26 May 2016
SRR1926479_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926479_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1000994
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA30910.3087930596986595No Hit
GTCCTACAGTGGACATTTCTAAATT29180.29151023882261035No Hit
GTCCTAAAGTGTGTATTTCTCATTT19880.19860258902650768No Hit
CTTTAGGACGTGAAATATGGCGAGG19740.19720397924463084No Hit
GTCCTACAGTGTGCATTTCTCATTT19030.19011102963654128No Hit
CTGTAGGACCTGGAATATGGCGAGA16310.1629380395886489No Hit
CCCATGTACTCTGCGTTGATACCAC13420.13406673766276322No Hit
CTGAAGGACCTGGAATATGGCGAGA11900.11888183145952923No Hit
GTACATGGGAAGCAGTGGTATCAAC11750.11738332097894692No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGAC1204.9112714E-1113.5119753
TAGGACC5950.012.9810584
GACTTAA853.787036E-612.3375371
GTCCTAC13600.012.1272371
TGTAGGA16300.012.0576292
TAGGACA3600.011.9193354
AGGACCT10500.011.8966315
TGTAGAA5000.011.63970852
GTAGGAC16300.011.4688443
GGACCTG9900.011.4618256
GTCCTAT1251.735134E-811.4402621
TCCTACA15200.011.3610182
CTAGGGT855.1200608E-511.2181984
CTAAATT7550.011.16861519
ATAACAC600.00570177311.1275093
GCCCTAA600.00572101611.1224761
CTGTAGG16650.011.1081621
ACGTTTT600.005991854211.05358119
GTCCTAA6050.011.0305551
ATTTCTA9400.010.88613915
Produced by FastQC (version 0.11.5)