FastQCFastQC Report
Thu 26 May 2016
SRR1926478_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926478_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences976806
Sequences flagged as poor quality0
Sequence length25
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATATAC1650.015.35351
GTAGGAC5050.014.5153533
TAGGACC2300.014.4671754
TGTAGGA5900.014.1339522
TTTAGGA3850.013.84808352
GTCCTAC4450.013.7827411
AGCCGTC450.007240711713.5849567
GTATTAG700.001469475613.3333031
GTACAGA1409.673386E-913.3333031
GTGTAGA1501.7753337E-913.3333031
AGGACCT4350.013.1741385
ATTACAC755.54178E-513.1288133
CTTAGAC500.00907359713.1288123
GTCCTAA2650.013.0817321
CTGTAGG6450.013.0232261
TAGGACG5550.012.7644134
GGCGAGG2900.012.63514419
CCATTAG854.4191597E-412.5489921
TTAGGAC3750.012.5453093
TGTAGAA2750.012.4277682
Produced by FastQC (version 0.11.5)