##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926478_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 976806 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.3148230047727 26.0 2.0 30.0 2.0 31.0 2 15.941855394008636 16.0 2.0 30.0 2.0 31.0 3 19.474716576270005 22.0 12.0 30.0 2.0 31.0 4 21.598906026375758 28.0 2.0 35.0 2.0 37.0 5 21.95942182992324 28.0 2.0 35.0 2.0 37.0 6 22.827948436025167 30.0 2.0 35.0 2.0 37.0 7 22.673750980235585 30.0 2.0 35.0 2.0 37.0 8 22.7712585713028 30.0 2.0 35.0 2.0 37.0 9 25.39226110404727 31.0 17.0 37.0 2.0 39.0 10 26.023043470248954 31.0 17.0 37.0 2.0 39.0 11 23.1960123095067 30.0 7.0 37.0 2.0 39.0 12 24.244432364256568 30.0 7.0 37.0 2.0 39.0 13 21.013399794841554 27.0 2.0 35.0 2.0 39.0 14 24.272315075869724 30.0 10.0 37.0 2.0 40.0 15 25.312713066873055 31.0 10.0 38.0 2.0 40.0 16 25.666538698574744 31.0 7.0 38.0 2.0 40.0 17 24.974460640086157 31.0 7.0 38.0 2.0 40.0 18 25.024325198657667 31.0 7.0 38.0 2.0 40.0 19 25.3977606607658 31.0 2.0 38.0 2.0 40.0 20 25.62413928661372 31.0 2.0 38.0 2.0 40.0 21 23.537935884914713 30.0 2.0 37.0 2.0 40.0 22 24.421809448344913 30.0 2.0 37.0 2.0 40.0 23 24.395671197760866 31.0 2.0 38.0 2.0 40.0 24 23.507174403105633 30.0 2.0 38.0 2.0 40.0 25 23.29143862752686 30.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.368998085712768 1101 2 10.136900561785728 1101 3 7.083652906715919 1101 4 8.662219665970515 1101 5 8.159500901728585 1101 6 7.799682410689808 1101 7 7.393820980159216 1101 8 5.850188400667207 1101 9 5.892007919668071 1101 10 5.513636618872713 1101 11 8.362120728045099 1101 12 5.947169247559653 1101 13 10.334309712476099 1101 14 7.886542894212578 1101 15 7.3136119470684555 1101 16 6.708378112294508 1101 17 2.6432605244341367 1101 18 5.16301027213434 1101 19 5.638034886043634 1101 20 4.007175389378382 1101 21 1.3417850834339369 1101 22 4.894565735659224 1101 23 6.109540572817693 1101 24 7.2275441189949134 1101 25 7.825918819330848 1102 1 5.549625613473477 1102 2 10.425436854355718 1102 3 6.805840036037228 1102 4 8.272928796635199 1102 5 8.043736249648497 1102 6 6.958988142900804 1102 7 6.7374535241404665 1102 8 6.9405761216453 1102 9 6.150967804194959 1102 10 5.467288741834427 1102 11 8.137165388128274 1102 12 5.497154241771703 1102 13 9.800054356838928 1102 14 7.483831133962198 1102 15 5.4270185467569085 1102 16 5.8996583203747655 1102 17 6.538223581613334 1102 18 6.645246992012005 1102 19 5.329608064269522 1102 20 5.735492740356619 1102 21 4.325166530563543 1102 22 5.496939508400139 1102 23 5.969579444954089 1102 24 7.5178110791081 1102 25 7.141659176325689 1103 1 4.63080881898988 1103 2 8.982298745971276 1103 3 6.140361998843979 1103 4 7.933856827710677 1103 5 7.119078387887676 1103 6 6.809690561741856 1103 7 7.0144318503457725 1103 8 6.815246851826174 1103 9 5.694939828390403 1103 10 5.019791192459778 1103 11 5.914204892068263 1103 12 4.818699492902791 1103 13 9.037990388836992 1103 14 7.282092631508757 1103 15 6.344143812036222 1103 16 6.031086025872185 1103 17 3.5898199688923 1103 18 2.602332217518761 1103 19 4.4903300599856735 1103 20 4.868088448351774 1103 21 7.1515451743305185 1103 22 2.732870387404642 1103 23 4.885270197834014 1103 24 6.324299223988227 1103 25 2.205302303393953 1104 1 5.497386922075133 1104 2 10.01482191206762 1104 3 7.009327872573678 1104 4 7.562993556785376 1104 5 7.488071830047389 1104 6 7.35990232174489 1104 7 7.311669493658535 1104 8 7.149368986863237 1104 9 6.095387126167353 1104 10 4.964205656236583 1104 11 8.057929111278632 1104 12 5.059936695120669 1104 13 9.218863412094407 1104 14 6.86765435550053 1104 15 6.138639085773505 1104 16 5.813000446573319 1104 17 6.190075666080666 1104 18 3.644993673752154 1104 19 4.391745009656059 1104 20 3.657506306491527 1104 21 2.667505572280806 1104 22 4.751791634738257 1104 23 5.132445639876558 1104 24 6.223401526985949 1104 25 5.324479592521307 1105 1 3.4138817604134566 1105 2 9.293140208293527 1105 3 6.476307202085575 1105 4 5.7700414132738125 1105 5 2.5411730844452727 1105 6 4.964488384066293 1105 7 6.061809987584088 1105 8 3.063935397064629 1105 9 4.096996079632358 1105 10 3.508791446084917 1105 11 6.735331557405598 1105 12 4.141638085073907 1105 13 5.077139951822684 1105 14 3.89591866799989 1105 15 4.375179086522806 1105 16 4.788510567244828 1105 17 5.414861178687655 1105 18 3.1121221822686422 1105 19 2.88862723657844 1105 20 2.17403758810266 1105 21 2.8774017941011287 1105 22 4.293826736081211 1105 23 1.780185305483986 1105 24 4.585232818474228 1105 25 5.243556113176471 1106 1 6.079606655806625 1106 2 10.553853889173505 1106 3 7.34355084134312 1106 4 8.595835413199463 1106 5 8.14054165709728 1106 6 7.259485499547555 1106 7 7.113523419252662 1106 8 6.977924417994256 1106 9 5.82860847741782 1106 10 5.182377859795125 1106 11 7.673531948158065 1106 12 4.777256590604058 1106 13 4.152762877068902 1106 14 5.177048408007682 1106 15 5.241264443494046 1106 16 5.190143659214851 1106 17 5.697740217530214 1106 18 5.805152937158898 1106 19 5.70222777685775 1106 20 5.126020677525492 1106 21 5.358652766507507 1106 22 5.772887814904987 1106 23 6.141391764052248 1106 24 6.926984953637682 1106 25 6.127335135634411 1107 1 3.7246905005376973 1107 2 -13.431961713663375 1107 3 -5.483336430671482 1107 4 1.7280179485073432 1107 5 4.214342169107471 1107 6 4.614282226602025 1107 7 4.491516780268842 1107 8 4.962625357496208 1107 9 3.3424131541042073 1107 10 2.8259091377382646 1107 11 5.391895720374777 1107 12 2.7259943262451145 1107 13 4.436631493783949 1107 14 3.6239916581699845 1107 15 3.3605946655692804 1107 16 3.155435982736698 1107 17 3.7839428902724066 1107 18 -1.1727865280476806 1107 19 1.4377983987418972 1107 20 1.861542303219096 1107 21 -1.2441994242346652 1107 22 1.6939090180064476 1107 23 -1.3627790366679982 1107 24 2.160825397543171 1107 25 2.855482834650811 1108 1 5.387015652883903 1108 2 10.62164059181213 1108 3 0.4152634608238408 1108 4 6.101283325395183 1108 5 7.576369182458194 1108 6 7.660178709159151 1108 7 7.61902072476223 1108 8 7.545561918760704 1108 9 6.139855164659355 1108 10 5.109396683757353 1108 11 7.279542944131659 1108 12 2.819922875749956 1108 13 3.9459544345593116 1108 14 5.295340285021737 1108 15 5.206360743423872 1108 16 5.287250914241046 1108 17 0.5330084061922342 1108 18 4.032930374293134 1108 19 5.171462555200996 1108 20 5.690450338040137 1108 21 5.328007451378163 1108 22 2.119503180653961 1108 23 4.846008846644679 1108 24 5.643459837989202 1108 25 6.213599366018453 1109 1 3.2560787286972612 1109 2 -13.431961713663375 1109 3 -5.4386711284876945 1109 4 1.7761753844268426 1109 5 1.1992058335020488 1109 6 2.2581583413906827 1109 7 3.1665424411518224 1109 8 3.401576016893827 1109 9 3.082821310679641 1109 10 2.414104721812741 1109 11 0.8976681385621426 1109 12 0.9612457209473959 1109 13 2.249039683139756 1109 14 3.2029605926235867 1109 15 3.1253074173378828 1109 16 2.8866471969010945 1109 17 3.498077580713037 1109 18 3.6975753057681366 1109 19 2.0375234533929216 1109 20 1.1954472442451625 1109 21 0.4037025296802348 1109 22 2.515290833506235 1109 23 2.9728069226802454 1109 24 3.9380817625689737 1109 25 4.323436950328638 1110 1 3.211739902140046 1110 2 8.844045502211548 1110 3 6.1742823513744085 1110 4 7.453075596922801 1110 5 8.26264290395384 1110 6 3.8261431392627294 1110 7 6.2991630320746275 1110 8 7.151207769026101 1110 9 5.442707722707812 1110 10 5.206235666117973 1110 11 2.6880553890898184 1110 12 5.570097294052356 1110 13 9.96217940904208 1110 14 7.692677828193084 1110 15 6.972713347394684 1110 16 6.581689457133393 1110 17 7.145494781116202 1110 18 7.121859620941233 1110 19 6.0593080342751335 1110 20 6.209558716530452 1110 21 4.120094249827549 1110 22 6.280209241191756 1110 23 6.749751431917293 1110 24 7.451696055650764 1110 25 7.846036491383014 1111 1 5.027108325305097 1111 2 9.16338989539503 1111 3 7.435369735275724 1111 4 7.746882759330877 1111 5 8.428256120730392 1111 6 7.504685515326781 1111 7 7.430712631897457 1111 8 7.550617340166042 1111 9 5.943471381302608 1111 10 5.078055338049378 1111 11 7.025986322244069 1111 12 6.27575474640701 1111 13 7.784320061266005 1111 14 5.907842131256963 1111 15 5.583432734575684 1111 16 5.441858945621892 1111 17 6.6102514531845 1111 18 6.650279833131535 1111 19 5.341711204443783 1111 20 4.922677876656508 1111 21 7.713773407144085 1111 22 7.0231540893451765 1111 23 6.95056174858297 1111 24 6.9820037337344445 1111 25 7.545172564522229 1112 1 -2.108811084678841 1112 2 7.163108708871837 1112 3 5.385600420139777 1112 4 -0.609626079158943 1112 5 3.3120113737423047 1112 6 2.6758441716748784 1112 7 5.1717510355414085 1112 8 5.467623249279843 1112 9 3.9288398895311616 1112 10 2.828866722046648 1112 11 -0.848643658674419 1112 12 3.2887522786805086 1112 13 7.317877084773759 1112 14 4.856992136572927 1112 15 3.074601938915748 1112 16 3.1616167055305695 1112 17 4.036509238274061 1112 18 4.350195057493199 1112 19 3.939541731507635 1112 20 3.5220850067633336 1112 21 -2.2574903094422822 1112 22 1.7028273813818622 1112 23 3.208567870335333 1112 24 4.912068028921539 1112 25 4.8208472722840945 1113 1 -17.842379159796213 1113 2 -13.431961713663375 1113 3 -7.528117302864919 1113 4 -6.479071501694154 1113 5 -1.8272169126426725 1113 6 -1.2484163917054012 1113 7 -20.118155067744976 1113 8 -9.617966539452553 1113 9 -5.439781002487617 1113 10 -4.8045370338218945 1113 11 -5.667070888721366 1113 12 -3.598897369206817 1113 13 -2.159769628841264 1113 14 -2.8378171357275406 1113 15 -3.1552278732908228 1113 16 -3.5740170972863297 1113 17 -3.171642170176643 1113 18 -3.753449308703985 1113 19 -3.3502323295252303 1113 20 -4.183677904034791 1113 21 -1.7142890183624715 1113 22 -2.6784020787120326 1113 23 -3.100642810415838 1113 24 -3.0545516893883224 1113 25 -3.005608126776938 1114 1 2.6372646061070952 1114 2 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2204 9 3.9150699420224164 2204 10 3.711560959094278 2204 11 5.045060162046049 2204 12 5.193390853933682 2204 13 2.90594613266995 2204 14 4.696590331804927 2204 15 4.752973130251089 2204 16 4.622393764579076 2204 17 -0.19391047005857942 2204 18 3.3241171021578815 2204 19 4.190754240722704 2204 20 4.630339215209602 2204 21 6.065273407847684 2204 22 5.39947351160674 2204 23 4.448206805876961 2204 24 5.777691521945798 2204 25 5.9207968319007485 2205 1 5.870915637891649 2205 2 9.991159673619864 2205 3 6.9818357318171635 2205 4 7.711054943392551 2205 5 7.707353174062529 2205 6 7.404863955115406 2205 7 7.470862273295488 2205 8 7.176141119506983 2205 9 5.922162639130878 2205 10 5.5675149892994895 2205 11 7.494923330931812 2205 12 6.916388035417462 2205 13 9.695512163066251 2205 14 7.289173471208876 2205 15 6.300140623819004 2205 16 5.658584058783035 2205 17 6.5701858645054365 2205 18 6.389766009215091 2205 19 4.75177272827824 2205 20 5.257810535271567 2205 21 -0.9856871397497535 2205 22 3.729123210305307 2205 23 5.144368027734451 2205 24 6.7135985996290195 2205 25 7.097484358772196 2206 1 4.270459067031652 2206 2 8.563432644425285 2206 3 6.04861521008845 2206 4 1.9842067798257084 2206 5 5.67548206548053 2206 6 6.0109431362163015 2206 7 6.10809709114967 2206 8 6.138791160605017 2206 9 5.080467270396664 2206 10 4.230629920697389 2206 11 3.6693856455330938 2206 12 4.861860762629686 2206 13 8.223044850145495 2206 14 0.7627366207491413 2206 15 2.7422872389716986 2206 16 3.598729016703306 2206 17 4.814455267935042 2206 18 -0.7732470922388153 2206 19 1.4498882087591696 2206 20 2.809153126477586 2206 21 3.656203569432577 2206 22 -1.463834217456995 2206 23 -2.793406555378418 2206 24 1.0335945397423636 2206 25 2.530785079901243 2207 1 1.2314060831551963 2207 2 -13.431961713663375 2207 3 -6.1111238265601795 2207 4 -4.170278385317431 2207 5 0.8076777334281147 2207 6 1.4907452759610642 2207 7 2.708479605320413 2207 8 2.8401434635468483 2207 9 0.4663880137091425 2207 10 0.13651575562021634 2207 11 -1.9102172594472222 2207 12 -2.7780181700466464 2207 13 -0.26844614353832696 2207 14 -5.721152093735938 2207 15 -1.9794502788097184 2207 16 -0.825690762553279 2207 17 0.3406336246643882 2207 18 0.1464449624417874 2207 19 -0.3349935022906756 2207 20 -0.27076698621835504 2207 21 -4.06107853545905 2207 22 -4.710886706786418 2207 23 -1.7853080023774481 2207 24 -0.27770105951429613 2207 25 0.26857603639042793 2208 1 4.996421745181163 2208 2 6.701522449232554 2208 3 6.3783165206644945 2208 4 8.237611190613539 2208 5 4.144595870162757 2208 6 6.357907137706363 2208 7 6.610351719585342 2208 8 6.597172035208079 2208 9 5.224364925114191 2208 10 4.757929029526519 2208 11 6.648653093179085 2208 12 6.352706137580515 2208 13 8.756785801022989 2208 14 3.1374289049964403 2208 15 4.977526651596055 2208 16 5.412883355202357 2208 17 6.177752626203446 2208 18 1.1194975238752 2208 19 3.6628757021489804 2208 20 4.76119766157607 2208 21 7.198349556298162 2208 22 6.5809050479848 2208 23 2.9238000628873273 2208 24 4.123638355860546 2208 25 5.369821737476457 2209 1 2.6025236097971884 2209 2 4.447236872076166 2209 3 4.8916878680248885 2209 4 0.2626723845757333 2209 5 4.352431363727394 2209 6 5.060132518135493 2209 7 5.571296906326914 2209 8 4.142294583116687 2209 9 3.9607029397634115 2209 10 2.8329909567497005 2209 11 -0.48119876143792695 2209 12 2.999017701506208 2209 13 1.901867819597154 2209 14 3.398769119428618 2209 15 3.784921655289022 2209 16 3.210225684092574 2209 17 0.586284317743214 2209 18 2.9906645687267748 2209 19 3.288208669296221 2209 20 3.257010074751303 2209 21 5.561680707624564 2209 22 4.301520947216076 2209 23 0.18403271993183168 2209 24 -3.6556654195002842 2209 25 -0.49181024816762786 2210 1 -2.9364751007556222 2210 2 -13.431961713663375 2210 3 -7.920341318855078 2210 4 -2.9905206099352384 2210 5 -1.6682001537250812 2210 6 -2.0871402539440247 2210 7 -1.9982288684829825 2210 8 -20.234841539452553 2210 9 -13.196833585513449 2210 10 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-6.569808996854977 2211 23 -8.528112648534348 2211 24 -21.02455168938832 2211 25 -20.810608126776938 2212 1 -0.8315580267599287 2212 2 -13.431961713663375 2212 3 -7.636819960407884 2212 4 -19.079696501694155 2212 5 -19.34409191264267 2212 6 -20.2009163917054 2212 7 -9.659848256796534 2212 8 -9.19283008178228 2212 9 -4.738658309935104 2212 10 -5.185151292256016 2212 11 -5.477844281464844 2212 12 -2.7220128211482546 2212 13 -5.876512945200272 2212 14 -3.595257858197307 2212 15 -3.647970553112593 2212 16 -3.659325817846483 2212 17 -3.87221266667569 2212 18 -2.8829949802507713 2212 19 -3.4849852735099525 2212 20 -3.5280811503746996 2212 21 -1.4665729935375182 2212 22 -2.727041082531258 2212 23 -2.741837511243336 2212 24 -1.7992175073386711 2212 25 -2.4363242311690456 2213 1 -17.842379159796213 2213 2 -13.431961713663375 2213 3 -8.522093509299236 2213 4 -5.809589263088256 2213 5 -19.34409191264267 2213 6 -10.568208616906475 2213 7 -20.118155067744976 2213 8 -10.524385775377485 2213 9 -8.342686498466172 2213 10 -7.173263575376858 2213 11 -6.451052121965336 2213 12 -5.293215398697431 2213 13 -18.401019628841265 2213 14 -11.970674039212795 2213 15 -7.171906827714416 2213 16 -6.19602782114692 2213 17 -4.913099944975571 2213 18 -5.006864188060554 2213 19 -5.959557061428715 2213 20 -5.757732863820312 2213 21 -3.972806109515286 2213 22 -4.902458714100774 2213 23 -4.608921965911819 2213 24 -3.7738814481014593 2213 25 -4.583396330530288 2214 1 -17.842379159796213 2214 2 -13.431961713663375 2214 3 -12.299564220673137 2214 4 -19.079696501694155 2214 5 -19.34409191264267 2214 6 -20.2009163917054 2214 7 -16.396693880530364 2214 8 -14.869544735799586 2214 9 -14.100631459108623 2214 10 -14.072139773547924 2214 11 -14.266119594961854 2214 12 -12.454891471185507 2214 13 -18.401019628841265 2214 14 -16.432281557340936 2214 15 -22.841477873290824 2214 16 -17.86144479896061 2214 17 -22.530392170176643 2214 18 -17.370403266086026 2214 19 -14.837775137744407 2214 20 -13.60734989794651 2214 21 -11.293481369960643 2214 22 -12.286804856489812 2214 23 -12.229799012851151 2214 24 -11.093044840073253 2214 25 -10.81745744184543 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 196357.0 3 3.0 4 1109.0 5 3593.0 6 4909.0 7 4710.0 8 4110.0 9 3887.0 10 3590.0 11 3409.0 12 3231.0 13 3587.0 14 4978.0 15 9825.0 16 14381.0 17 15310.0 18 15919.0 19 18725.0 20 22211.0 21 25363.0 22 27024.0 23 28878.0 24 30235.0 25 30613.0 26 26001.0 27 21004.0 28 22564.0 29 25930.0 30 24935.0 31 28404.0 32 36637.0 33 44548.0 34 53432.0 35 60021.0 36 58321.0 37 59801.0 38 39251.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.234274435362394 17.480627842076185 12.069173124052641 26.21592459850878 2 14.554697312536083 19.460352228081433 39.88755461851071 26.097395840871773 3 19.317748801196792 23.42402296317446 28.826752445645386 28.431475789983356 4 11.906373499012007 15.718455502355983 37.08995953032376 35.285211468308255 5 13.58213879045217 36.853288495851466 35.576389849607615 13.988182864088747 6 33.71076014930431 35.74879285108265 17.318290739623563 13.222156259989474 7 28.701237556410376 30.389060818767593 22.010348911648116 18.89935271317392 8 25.734899522337486 36.44693599677124 19.60467550438412 18.213488976507165 9 26.419209051127808 15.072300392502486 19.060863505467367 39.44762705090235 10 14.698067507302722 28.96483258313669 34.126220152395604 22.210879757164985 11 34.34961247548791 21.545450794484967 24.047910147300573 20.057026582726547 12 24.14039218331627 23.88340472478297 30.87929531906375 21.09690777283701 13 28.797083987934585 20.449682068259197 24.984710712023055 25.768523231783163 14 20.878929817419724 21.3848074302271 27.00339091044436 30.73287184190882 15 23.285882033952664 30.441135666124037 22.775885188463562 23.497097111459738 16 22.63051332936918 27.34007238975982 27.081099977926087 22.948314302944915 17 21.991938426622177 27.60255726100557 28.15644767676086 22.2490566356114 18 21.970637857899213 26.89275973762552 29.550206267279954 21.586396137195308 19 23.599464383365113 26.39388199384124 26.399911839497985 23.606741783295664 20 23.88754238410392 27.1413275681713 26.963322030905623 22.007808016819162 21 22.6742585636448 25.532145064356538 26.178870148356893 25.614726223641775 22 23.060326200708296 27.18130973864165 27.10758500472234 22.650779055927707 23 22.541301139624498 27.03103222103796 27.53945642371075 22.888210215626795 24 23.31791466334653 26.503038892647556 27.35701331844375 22.822033125562157 25 23.09772805535969 26.560783333060034 27.35526704163455 22.986221569945723 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 902.0 1 902.0 2 1923.5 3 2945.0 4 2945.0 5 2945.0 6 4247.5 7 5550.0 8 5550.0 9 5550.0 10 6447.0 11 7344.0 12 7344.0 13 7344.0 14 8384.5 15 9425.0 16 9425.0 17 9425.0 18 13182.0 19 16939.0 20 16939.0 21 16939.0 22 24010.0 23 31081.0 24 31081.0 25 31081.0 26 41411.0 27 51741.0 28 51741.0 29 51741.0 30 63961.0 31 76181.0 32 76181.0 33 76181.0 34 86468.5 35 96756.0 36 96756.0 37 96756.0 38 103707.0 39 110658.0 40 110658.0 41 110658.0 42 114805.0 43 118952.0 44 118952.0 45 118952.0 46 120611.0 47 122270.0 48 122270.0 49 122270.0 50 115307.0 51 108344.0 52 108344.0 53 108344.0 54 96434.0 55 84524.0 56 84524.0 57 84524.0 58 72079.0 59 59634.0 60 59634.0 61 59634.0 62 48721.5 63 37809.0 64 37809.0 65 37809.0 66 29053.5 67 20298.0 68 20298.0 69 20298.0 70 14972.0 71 9646.0 72 9646.0 73 9646.0 74 6708.5 75 3771.0 76 3771.0 77 3771.0 78 2602.0 79 1433.0 80 1433.0 81 1433.0 82 937.5 83 442.0 84 442.0 85 442.0 86 281.5 87 121.0 88 121.0 89 121.0 90 78.0 91 35.0 92 35.0 93 35.0 94 20.0 95 5.0 96 5.0 97 5.0 98 2.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.279402460672845 2 36.34222148512601 3 5.700108312193004 4 13.78922733889841 5 13.419757863895185 6 14.811538831661558 7 9.327235909689334 8 7.66928131072086 9 0.10401246511589815 10 1.518827689428607 11 5.7157716066445134 12 5.906495250848173 13 16.13472890215662 14 1.6683967952694803 15 2.964457630276636 16 3.069903338022084 17 2.928319441117274 18 1.5798428756580118 19 1.527836643100063 20 0.2742612146117039 21 4.420427392952131 22 1.6868242005065488 23 0.10708369932207623 24 9.059014789016448 25 0.11035968247533287 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 976806.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 92.01363581762037 #Duplication Level Percentage of deduplicated Percentage of total 1 95.9076166679099 88.24808512217 2 3.044641969642927 5.602971547795333 3 0.5478890542511592 1.5123979171897985 4 0.18399744953136074 0.6772109725019844 5 0.09801401187610932 0.450931279689612 6 0.05426410630009376 0.2995822629039279 7 0.040004489613349334 0.2576670976846751 8 0.02438951579369016 0.1795334419206965 9 0.01493118757841148 0.12364855705480778 >10 0.07347134760450325 1.2128690116381453 >50 0.005506840141873387 0.36519091832974443 >100 0.0047001689479299474 0.7448140644587762 >500 5.731908086553631E-4 0.32509780666247734 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 1.0237447353926983E-4 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATATAC 165 0.0 15.3535 1 GTAGGAC 505 0.0 14.515353 3 TAGGACC 230 0.0 14.467175 4 TGTAGGA 590 0.0 14.133952 2 TTTAGGA 385 0.0 13.8480835 2 GTCCTAC 445 0.0 13.782741 1 AGCCGTC 45 0.0072407117 13.584956 7 GTATTAG 70 0.0014694756 13.333303 1 GTACAGA 140 9.673386E-9 13.333303 1 GTGTAGA 150 1.7753337E-9 13.333303 1 AGGACCT 435 0.0 13.174138 5 ATTACAC 75 5.54178E-5 13.128813 3 CTTAGAC 50 0.009073597 13.128812 3 GTCCTAA 265 0.0 13.081732 1 CTGTAGG 645 0.0 13.023226 1 TAGGACG 555 0.0 12.764413 4 GGCGAGG 290 0.0 12.635144 19 CCATTAG 85 4.4191597E-4 12.548992 1 TTAGGAC 375 0.0 12.545309 3 TGTAGAA 275 0.0 12.427768 2 >>END_MODULE