FastQCFastQC Report
Thu 26 May 2016
SRR1926478_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926478_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences976806
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA17720.18140756711158612No Hit
GTCCTACAGTGGACATTTCTAAATT16860.17260336238720891No Hit
CCCATGTACTCTGCGTTGATACCAC16190.16574427266007785No Hit
GTACATGGGAAGCAGTGGTATCAAC14680.1502857271556481No Hit
GTCCTAAAGTGTGTATTTCTCATTT13060.13370106244228638No Hit
CTTTAGGACGTGAAATATGGCGAGG12750.13052745376256902No Hit
GTATCAACGCAGAGTACTTTTTTTT11230.1149665337846No Hit
GTCCTACAGTGTGCATTTCTCATTT10390.10636707800730134No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGC400.00534619514.22214110
TAGGACC4000.014.0694444
AAGGCGT500.001453722213.3540496
GTTCTAA853.775247E-612.34089851
GTCCTAA5200.012.2869621
AAAGGCG550.00297525212.1400455
TACACGG550.00297525212.1400455
GGACCTG6850.011.9754766
AGGACCT6800.011.9232585
AATGCCG802.9494146E-511.84086418
GATATAC2950.011.6373331
CTAAATT5550.011.43550119
GTAGGAC8350.011.4264263
TCCAACG1251.9070285E-811.3672318
TGTAGGA8350.011.3133282
AGACAGT1701.0913936E-1111.221896
TCCTAAA5800.011.187232
CGTTTCC855.4278054E-511.15404914
CTTAGGC600.0056873811.1312433
TTAGGCA600.00569830711.1283744
Produced by FastQC (version 0.11.5)