FastQCFastQC Report
Thu 26 May 2016
SRR1926477_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926477_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences841157
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT15890.1889064704924289No Hit
CTGTAGGACGTGGAATATGGCAAGA12510.14872372220643706No Hit
GTCCTAAAGTGTGTATTTCTCATTT12290.14610827705172758No Hit
GTCCTACAGTGTGCATTTCTCATTT9910.11781391583259725No Hit
CTTTAGGACGTGAAATATGGCGAGG9100.10818432230843945No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCTAC654.7033347E-516.3330611
TAGGACC4750.015.7568914
TGTAGGA10050.015.5577252
CTGTAGG9350.015.1866881
CTAGGAC1450.014.2690563
GTCCTAA5450.014.1228521
GTGTAAG955.369746E-613.9690651
GTCCTAG1151.8191167E-713.8475941
GTCCTAC11350.013.6798371
TCCTAAA5200.013.5960992
GTAGGAC11500.013.4462183
TTTAGGA6150.013.2644862
GACTGCT1151.9772415E-913.23867
CTACACT1700.013.2079834
TTTAGAC755.7882717E-513.0674513
AGGACCT7750.012.9841345
ACTGATC603.34046E-412.9727658
ACAGCGT450.00981841912.9727658
TCAGAAC1108.393363E-812.869463
GGACCTG7250.012.7246186
Produced by FastQC (version 0.11.5)