FastQCFastQC Report
Thu 26 May 2016
SRR1926477_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926477_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences841157
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA36240.43083514730305994No Hit
GTCCTACAGTGGACATTTCTAAATT34790.41359698605611084No Hit
CTTTAGGACGTGAAATATGGCGAGG27700.3293083217520629No Hit
GTCCTAAAGTGTGTATTTCTCATTT27520.32716841208002784No Hit
GTCCTACAGTGTGCATTTCTCATTT22040.26202005095362696No Hit
CTGTAGGACCTGGAATATGGCGAGA19190.22813814781307176No Hit
CTGAAGGACCTGGAATATGGCGAGA16670.1981794124045808No Hit
CCCATGTACTCTGCGTTGATACCAC15630.1858154898550449No Hit
GTACATGGGAAGCAGTGGTATCAAC13230.15728336089457734No Hit
GTCCTTCAGTGTGCATTTCTCATTT9950.1182894513152717No Hit
GAATATGGCAAGAAAACTGAAAATC9650.11472293519521326No Hit
GATATACACTGTTCTACAAATCCCG8840.10509334167105547No Hit
CTGTAGGACATGGAATATGGCAAGA8690.10331008361102623No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6150.014.4210464
ACGAATG400.005368467414.21290419
CCAACGA1101.9281288E-1013.78220919
CCTCTAA655.268897E-513.2004341
GGACTTG1550.012.9196446
TGTAGGA19900.012.8979912
TAAGGTC603.9583634E-412.7153315
GACTTGG1352.7284841E-1112.6638067
CTGTAGG20050.012.648131
GTAGGAC19500.012.5708723
TCTACAC1001.3659883E-712.3996733
GTCCTAC17350.012.3635181
GGCGAGG8100.012.28275719
TCCAACG1551.8189894E-1212.22615318
GTAGCAC550.002975582812.1395413
CCCGTTT1251.4515535E-912.13555912
GTCTAAG550.00298627312.1337321
TAGGACT2150.011.9760684
TGTCGAG802.9400768E-511.84408718
TCCTACA18550.011.7277262
Produced by FastQC (version 0.11.5)