##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926477_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 841157 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.072468041043468 31.0 28.0 31.0 16.0 34.0 2 28.537496567228235 31.0 28.0 33.0 22.0 34.0 3 28.940460579891745 31.0 28.0 33.0 23.0 34.0 4 32.67296592669383 35.0 33.0 37.0 26.0 37.0 5 32.32484304356975 35.0 33.0 37.0 25.0 37.0 6 32.43136893588236 35.0 33.0 37.0 25.0 37.0 7 32.27245567712092 35.0 33.0 37.0 22.0 37.0 8 32.238774687721794 35.0 33.0 37.0 22.0 37.0 9 33.453006989182754 37.0 33.0 39.0 19.0 39.0 10 33.301570337047664 37.0 33.0 39.0 18.0 39.0 11 33.553065598931 37.0 33.0 39.0 23.0 39.0 12 33.34331403055553 37.0 33.0 39.0 19.0 39.0 13 33.49016057644411 37.0 33.0 39.0 22.0 39.0 14 34.091217216286616 38.0 33.0 40.0 17.0 41.0 15 34.24887387253509 38.0 33.0 40.0 19.0 41.0 16 34.227336870524766 38.0 33.0 40.0 19.0 41.0 17 34.15189792155329 38.0 33.0 40.0 18.0 41.0 18 34.1165525579648 38.0 33.0 40.0 17.0 41.0 19 34.146044079761566 39.0 34.0 40.0 18.0 41.0 20 34.13200508347431 39.0 34.0 40.0 18.0 41.0 21 34.04541007207929 39.0 34.0 40.0 18.0 41.0 22 33.97112191897589 39.0 34.0 40.0 17.0 41.0 23 33.614566602905285 39.0 33.0 40.0 10.0 41.0 24 33.70937767860221 39.0 33.0 40.0 2.0 41.0 25 33.49692625752386 38.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5014970367306226 1101 2 0.8274860087980862 1101 3 0.6642848544287183 1101 4 0.49585496526269424 1101 5 0.5414051494657031 1101 6 0.21987399398522456 1101 7 0.5602972658270318 1101 8 0.6956570152993251 1101 9 0.8876603444890989 1101 10 1.0172291887832614 1101 11 0.7951091264535322 1101 12 0.7410399401498964 1101 13 0.5733251200505123 1101 14 1.001003526933765 1101 15 0.9092377954089841 1101 16 0.7960718516226848 1101 17 0.8958271844820445 1101 18 0.7776145298621913 1101 19 0.8033421422988596 1101 20 0.7395621340806713 1101 21 0.7039036071296891 1101 22 0.7077586222212133 1101 23 0.9538464953754584 1101 24 0.9181637725868654 1101 25 0.9074334086507463 1102 1 0.3426455780192512 1102 2 0.44924163351015167 1102 3 0.5294624101117229 1102 4 0.2270007799880176 1102 5 0.30626585968843045 1102 6 -0.18729216654354275 1102 7 0.038080945935341504 1102 8 0.28550197726846704 1102 9 0.701303973262803 1102 10 0.5247911710888076 1102 11 0.31036612855016443 1102 12 0.45684930636495835 1102 13 0.3501639049434715 1102 14 0.6288751152038969 1102 15 0.3911033288281871 1102 16 0.4698846213409098 1102 17 0.47491491400696617 1102 18 0.4984788504308213 1102 19 0.5037530071835405 1102 20 0.36982510641075805 1102 21 0.5870755404511172 1102 22 0.556552823103118 1102 23 0.8709885869315741 1102 24 0.74350106727195 1102 25 0.6464716839584241 1103 1 0.039473941519482025 1103 2 -0.09579600184382642 1103 3 -0.17857336663622903 1103 4 -0.2453673450131646 1103 5 0.05408109950155193 1103 6 -0.3850224172001049 1103 7 -0.14653318060491216 1103 8 -0.06439053366478475 1103 9 0.21567460917832904 1103 10 0.21652727550351614 1103 11 0.02647634785811448 1103 12 -0.01596787146563372 1103 13 -0.019229315965937133 1103 14 0.3691098705693676 1103 15 0.00786604553163528 1103 16 0.11929536949128305 1103 17 0.0716413661492794 1103 18 0.06765000289890821 1103 19 0.1329998652317954 1103 20 0.19074331072884831 1103 21 0.29532969868031245 1103 22 0.2611436556186959 1103 23 0.5588070599250656 1103 24 0.4395438246643053 1103 25 0.35376723256862874 1104 1 0.697495788061925 1104 2 0.6347393049161951 1104 3 0.5150358919255034 1104 4 0.34372304708721657 1104 5 0.5041250436961207 1104 6 0.2126610959720736 1104 7 0.4492190036800565 1104 8 0.46399224611941037 1104 9 0.9338353784602305 1104 10 1.018218942354828 1104 11 0.7523605862941452 1104 12 0.6905716893878449 1104 13 0.6313386350529058 1104 14 0.8924501463468886 1104 15 0.7880361354141243 1104 16 0.5519148866221357 1104 17 0.6645076354718 1104 18 0.7511225543948541 1104 19 0.8075967972623133 1104 20 0.8806745509891201 1104 21 0.9880840764822167 1104 22 0.855171307226172 1104 23 1.0626863352520601 1104 24 0.8758210497545917 1104 25 1.1491848501285773 1105 1 0.6847684396847953 1105 2 0.2501682412496038 1105 3 0.43522471725755096 1105 4 0.2036489496969054 1105 5 0.3135947118859761 1105 6 -0.14645436643109377 1105 7 -0.08182555967719196 1105 8 0.05488094445836822 1105 9 0.5040497109505608 1105 10 0.5106183518912459 1105 11 0.1066143119673697 1105 12 0.23394290356475977 1105 13 0.35407401547136175 1105 14 0.5599788102421357 1105 15 0.6362468353956743 1105 16 0.4687226567176168 1105 17 0.4059310796255957 1105 18 0.24262016829969468 1105 19 0.4589944448148984 1105 20 0.36384254004817507 1105 21 0.3172204873175133 1105 22 0.4112563021428315 1105 23 0.8206767691631924 1105 24 0.642778787468643 1105 25 0.884011708985355 1106 1 0.6696117923201363 1106 2 0.8920487623500897 1106 3 0.7393335182210379 1106 4 0.6935409337176424 1106 5 0.6949034848911211 1106 6 -0.34849908902520355 1106 7 -0.1977854717159495 1106 8 0.048337617093068275 1106 9 0.21080702716479038 1106 10 0.23639271731752842 1106 11 -0.12002031013800263 1106 12 0.020593870547031656 1106 13 -0.1695454069738105 1106 14 0.17891198442798384 1106 15 -0.05702864567599164 1106 16 0.07887852447465349 1106 17 -0.017102830617375275 1106 18 -0.06784109057842613 1106 19 0.013626120541140097 1106 20 0.07810021918318455 1106 21 -0.2478337357362861 1106 22 -0.1341813803256784 1106 23 0.3359409486127376 1106 24 0.12715975213897934 1106 25 0.036290582694292084 1107 1 1.119540374875271 1107 2 1.1802584154540412 1107 3 1.1344370009105589 1107 4 1.0203318482569657 1107 5 1.127731483697751 1107 6 0.8105109939035202 1107 7 1.0687111659877075 1107 8 1.0394163769377158 1107 9 1.3112389640956579 1107 10 0.7843216114164164 1107 11 0.8667364401966466 1107 12 1.0803865091377958 1107 13 1.099092433975187 1107 14 1.5067167362622627 1107 15 1.3411295499048421 1107 16 1.278617672587309 1107 17 1.1187540071079312 1107 18 1.3067361471901862 1107 19 1.336270598956176 1107 20 1.14151389384174 1107 21 1.3936995347602519 1107 22 1.4243558425521954 1107 23 1.4264496889991065 1107 24 1.4047320342497684 1107 25 1.5835633867728802 1108 1 1.1974193875007266 1108 2 1.1253211993722942 1108 3 0.9655908378200166 1108 4 0.6760203425073499 1108 5 -0.7379067250463294 1108 6 0.28966070702376356 1108 7 0.6971735522916447 1108 8 0.8113855978814826 1108 9 1.0234080909579717 1108 10 1.0363362936168201 1108 11 0.8336941928173616 1108 12 0.7700821007006837 1108 13 0.566255775171534 1108 14 0.9422928306458758 1108 15 0.8391689473715118 1108 16 0.8525059491455735 1108 17 1.0108122792368164 1108 18 0.9664870545268229 1108 19 0.9685224247887447 1108 20 0.9693495568927943 1108 21 1.0239280943182791 1108 22 0.8927273921835948 1108 23 1.3037630969795444 1108 24 1.1847050459206727 1108 25 1.2948650929434535 1109 1 0.49915273507831515 1109 2 -0.3165025992139654 1109 3 0.3746670979913027 1109 4 0.6020169951078174 1109 5 -0.9500014315256848 1109 6 0.27755887841774296 1109 7 0.689885836179613 1109 8 0.5382734065811619 1109 9 0.8304768016749975 1109 10 -1.6976415921798775 1109 11 -0.20405530418243956 1109 12 0.4406209786096227 1109 13 0.606147696899356 1109 14 0.7897880677236699 1109 15 0.8206674428222485 1109 16 0.8191476883689077 1109 17 0.7635600847315374 1109 18 0.8098016032510671 1109 19 0.6536269419975795 1109 20 0.7518108748423344 1109 21 0.6193463412067999 1109 22 0.7935468818832163 1109 23 0.8164765382131023 1109 24 0.7748836332468514 1109 25 0.9962979758022783 1110 1 0.5797243609565044 1110 2 -0.41509631875179664 1110 3 0.30846551155097757 1110 4 0.5503461121835471 1110 5 0.8063388098932336 1110 6 0.47157672528420846 1110 7 0.7803035392940103 1110 8 0.8348203033450972 1110 9 1.1894520279318286 1110 10 0.6505047812513567 1110 11 0.6168935064570888 1110 12 0.8511327312808987 1110 13 0.8618732337968993 1110 14 1.247887392887563 1110 15 0.9905241370413975 1110 16 0.7128557166401492 1110 17 0.9274534169374036 1110 18 0.9578610677511179 1110 19 1.1926117047479465 1110 20 1.1050487023069664 1110 21 1.0300953317713066 1110 22 1.025808416694943 1110 23 1.525086394625454 1110 24 1.3381798776065779 1110 25 1.3840029458186933 1111 1 0.06526597594818995 1111 2 0.16447277579100117 1111 3 0.17568284107465004 1111 4 0.007055062112286237 1111 5 0.1717195381491976 1111 6 0.009507362395495988 1111 7 0.13486353795576633 1111 8 0.11046662203409596 1111 9 0.397683811890829 1111 10 0.465972472170769 1111 11 -0.13600892761461836 1111 12 -0.0480551680143364 1111 13 -0.009587910876170724 1111 14 0.1408408382400239 1111 15 -0.04201053998860971 1111 16 0.06795101312869889 1111 17 -0.047757461132249546 1111 18 0.2647451743776159 1111 19 0.09691002548334637 1111 20 -0.09892067977254015 1111 21 0.0794765562929669 1111 22 -0.1910912888447882 1111 23 0.20899349257241084 1111 24 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2202 19 0.8168248154076352 2202 20 0.8161939364982942 2202 21 0.9590993868169164 2202 22 0.8868620345252722 2202 23 0.9533537290208329 2202 24 0.8575741000911918 2202 25 1.0165818425047561 2203 1 -0.30252777879063686 2203 2 -0.07379811736032593 2203 3 -0.17762312776884315 2203 4 -0.5769019081357598 2203 5 -0.26560889596519743 2203 6 -0.4107311733461465 2203 7 -0.11200202323140473 2203 8 -0.1858816240856811 2203 9 -0.019467374525220293 2203 10 -0.06746919088650571 2203 11 -0.1646379016565973 2203 12 -0.18008022631446607 2203 13 -0.33725711989708174 2203 14 0.2812985002493704 2203 15 0.27842840096165133 2203 16 0.03501179729056503 2203 17 0.30904125688633854 2203 18 0.30161217932368345 2203 19 0.1293965129518071 2203 20 0.01948236990519092 2203 21 0.1241326883003353 2203 22 0.1623624023495296 2203 23 0.35058560486816503 2203 24 0.39158727411857086 2203 25 0.33631131551547355 2204 1 -0.13760498298654156 2204 2 -0.06819024567889187 2204 3 -0.22554640503293655 2204 4 -0.3708806253291925 2204 5 -0.34149002462874734 2204 6 -0.417640313883183 2204 7 -0.3480899143228804 2204 8 -0.13334088005938582 2204 9 -0.014516297684956214 2204 10 -0.03340589595624266 2204 11 -0.11583131589037521 2204 12 -0.21884818155006514 2204 13 -0.3490690017232936 2204 14 0.10985040504918686 2204 15 0.2005958428926462 2204 16 -0.1479723827678896 2204 17 -0.20919072343066603 2204 18 -0.29744916322941606 2204 19 -0.1939550552051088 2204 20 -0.29638476988122164 2204 21 -0.12375950257012391 2204 22 -0.20433383430133034 2204 23 -0.12041780326206464 2204 24 -0.34068495170512136 2204 25 -0.24231735751253325 2205 1 0.5588522162453522 2205 2 0.45562192069709084 2205 3 0.5116794286967803 2205 4 0.3360376136645158 2205 5 0.3426810022725775 2205 6 0.23030465026599956 2205 7 0.17224551544651234 2205 8 0.34984270784118365 2205 9 0.7123850288834035 2205 10 0.5903780830441647 2205 11 0.5094747813913045 2205 12 0.5786440228788763 2205 13 0.5317591786054408 2205 14 0.8124145753713989 2205 15 0.7446483066454448 2205 16 0.596689632981807 2205 17 0.654834257630938 2205 18 0.660195781540942 2205 19 0.5266745802968913 2205 20 0.6150478534825936 2205 21 0.6760810625867606 2205 22 0.6054665638453471 2205 23 1.0592229838703986 2205 24 1.0120307257608303 2205 25 1.0811777008881833 2206 1 0.18315690265892215 2206 2 0.2602188126453626 2206 3 0.4502328287802193 2206 4 0.23182662075672056 2206 5 0.4102680902992759 2206 6 0.15950347518603536 2206 7 0.25560839960726867 2206 8 0.20811693951966959 2206 9 0.20812449488256846 2206 10 0.2901051724614945 2206 11 0.24677227130926127 2206 12 0.12997902496481828 2206 13 -0.026980913175947308 2206 14 0.29010109324096334 2206 15 0.14916784495722624 2206 16 0.2739554861315767 2206 17 0.31467555230484123 2206 18 0.29043149284674286 2206 19 0.1080984086934933 2206 20 0.18927431711769316 2206 21 0.20780735239345915 2206 22 0.04497080525823094 2206 23 0.6222540296220345 2206 24 0.4239875120214336 2206 25 0.5328177718101728 2207 1 -1.056722141351571 2207 2 0.021321625236442543 2207 3 0.2156547174424155 2207 4 0.4520193488243365 2207 5 0.6592133686437833 2207 6 0.5739835830263189 2207 7 0.509612334320181 2207 8 0.39428715228562794 2207 9 0.7362904814754003 2207 10 0.6302428874134023 2207 11 0.33552008535764344 2207 12 0.4225467894649668 2207 13 0.20072238032448553 2207 14 -4.8572156511484295 2207 15 -1.7044608347280032 2207 16 -0.5676267785143025 2207 17 -0.4569125415453499 2207 18 0.0625460367115025 2207 19 0.29923255325775955 2207 20 0.5875073395601262 2207 21 0.6059584016502413 2207 22 0.6029641016732654 2207 23 0.9805588241425838 2207 24 0.6825473342307049 2207 25 0.6814060113904716 2208 1 0.669003502867227 2208 2 0.768476089700993 2208 3 0.6643488626496392 2208 4 0.5800723667901835 2208 5 0.7369058505081227 2208 6 0.8026762477712879 2208 7 0.9644543167462203 2208 8 0.9139947174477356 2208 9 1.334555728059449 2208 10 1.0614096658404577 2208 11 1.0115897193351202 2208 12 0.8564097509119861 2208 13 0.8088592621226383 2208 14 1.296107115748434 2208 15 1.0739287706852636 2208 16 1.1702595568191043 2208 17 0.9791959327947524 2208 18 0.9431532874931037 2208 19 0.6951952456089998 2208 20 0.6827437345474578 2208 21 0.9562218368451809 2208 22 0.8483973110541712 2208 23 1.236704919641106 2208 24 1.0916417267622052 2208 25 1.066153331318361 2209 1 -0.4681883155532631 2209 2 -0.14213622693056038 2209 3 0.2727448104808943 2209 4 0.42045172060422686 2209 5 0.5181946206892292 2209 6 0.4595111906751086 2209 7 0.8309106652379441 2209 8 0.6710789611593455 2209 9 0.913736832403444 2209 10 0.6275616026408528 2209 11 0.7860881343547916 2209 12 0.7745618439322186 2209 13 0.7366310249549031 2209 14 1.0865451604837162 2209 15 0.8463503298893258 2209 16 0.8741774199054504 2209 17 0.9295805156335746 2209 18 0.8569099620937095 2209 19 0.7390661125639468 2209 20 0.6714243062252336 2209 21 0.7066550214080607 2209 22 0.7488358114306166 2209 23 0.8793572719428511 2209 24 0.7683157198494754 2209 25 0.7877761081691901 2210 1 -0.15083075540119495 2210 2 -0.04762977143883518 2210 3 0.43913738696905824 2210 4 0.4724551700109032 2210 5 0.6375173709837654 2210 6 0.6990530511684696 2210 7 0.8165713359289128 2210 8 0.8309419565560887 2210 9 1.099601552503529 2210 10 0.9883986910501221 2210 11 0.6252680500629495 2210 12 0.43555561313775826 2210 13 0.5678627303464268 2210 14 0.911677246038721 2210 15 0.5615022130953307 2210 16 0.8632097641659513 2210 17 0.7521012545839341 2210 18 0.8239875615981589 2210 19 0.8281166755216915 2210 20 0.9153920955685138 2210 21 0.7328437914030772 2210 22 0.9319871602215031 2210 23 1.038824314319541 2210 24 0.9984494450872319 2210 25 1.0770258622167717 2211 1 -1.0770456457242972 2211 2 -0.20911719528828598 2211 3 0.4912158195242995 2211 4 0.6202184046249712 2211 5 0.9624716303882295 2211 6 0.8073014376815308 2211 7 0.9997408073485801 2211 8 0.9677334799692403 2211 9 1.160980907912979 2211 10 1.3008411383227738 2211 11 0.9545008144033815 2211 12 1.3872515336798088 2211 13 1.239893110144159 2211 14 1.257498767240243 2211 15 1.142391189109162 2211 16 1.3350994036697372 2211 17 1.4573046243086836 2211 18 1.3212044222853763 2211 19 1.2755501757328034 2211 20 1.3607717927758216 2211 21 1.4278646008608291 2211 22 1.455521953232747 2211 23 1.7806226736898338 2211 24 1.6485600267467646 2211 25 1.6866861754644589 2212 1 1.0526282114919354 2212 2 0.07968609182008635 2212 3 0.4627489474133597 2212 4 0.4413406439675498 2212 5 0.6535990058376413 2212 6 0.5062998968494838 2212 7 0.49850814170913793 2212 8 0.5958023038007809 2212 9 0.9864046367265402 2212 10 0.8272022066329967 2212 11 0.7269584415220223 2212 12 0.9000789604902906 2212 13 0.8204401055216621 2212 14 0.949550641919231 2212 15 0.8112407011790168 2212 16 0.32267002513867027 2212 17 0.8310077573338503 2212 18 0.7702670827887133 2212 19 0.570465429970092 2212 20 0.7372997845580542 2212 21 0.8805225361458824 2212 22 0.84792051512283 2212 23 1.1860797871977908 2212 24 1.1520782599260144 2212 25 0.8698140645245473 2213 1 0.8037860622851163 2213 2 0.5897592709370265 2213 3 -0.09681836264017463 2213 4 -0.7998889125375328 2213 5 -1.3759734611335297 2213 6 -0.20354244881134065 2213 7 -0.004919980681705738 2213 8 0.19736656881723036 2213 9 -0.5361102201076022 2213 10 -0.5216620436494068 2213 11 -0.3370204887859174 2213 12 -0.5806403067362709 2213 13 -0.5109458774850637 2213 14 -7.8558813113247155 2213 15 -3.0722190994201704 2213 16 -1.7186724924194081 2213 17 -0.8774937897414361 2213 18 -4.68736515531354 2213 19 -2.416892754794901 2213 20 -4.380575074240632 2213 21 -1.4353128845112622 2213 22 -1.0422003040903718 2213 23 -4.160312826131612 2213 24 -1.747769488219955 2213 25 -1.294619165670433 2214 1 0.2724974818124686 2214 2 0.13557166232659768 2214 3 -1.789258491597689 2214 4 -2.0729274886218825 2214 5 -0.5020595654346494 2214 6 -0.6055970509680435 2214 7 -0.6996156959453863 2214 8 -0.6309836058371161 2214 9 -5.630231945704267 2214 10 -1.875590157440957 2214 11 -1.3938899339292377 2214 12 -1.4807245621002387 2214 13 -1.6199219992808729 2214 14 -2.919954882640443 2214 15 -2.2584863527538133 2214 16 -1.7462529592175287 2214 17 -2.155241011947407 2214 18 -1.42384187684371 2214 19 -4.475382945481172 2214 20 -2.5049658318952908 2214 21 -4.224769554435021 2214 22 -6.380090643393743 2214 23 -6.73503387651515 2214 24 -6.456449253475149 2214 25 -4.375621336416266 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 31896.0 3 17.0 4 614.0 5 3696.0 6 4386.0 7 3601.0 8 2634.0 9 2154.0 10 1862.0 11 1783.0 12 1761.0 13 1614.0 14 1731.0 15 1725.0 16 1732.0 17 1865.0 18 2067.0 19 2475.0 20 3108.0 21 4163.0 22 5363.0 23 6476.0 24 7934.0 25 9187.0 26 10703.0 27 11679.0 28 13720.0 29 16473.0 30 20015.0 31 24952.0 32 32452.0 33 41367.0 34 53810.0 35 70397.0 36 93870.0 37 136716.0 38 211159.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.48807767359954 19.679453619532623 8.874756080148494 30.957712626719335 2 12.707282846541718 21.377604414422986 41.108362667681476 24.806750071353818 3 18.6434147246443 24.882979178856335 28.891655844619184 27.581950251880183 4 11.786279149968488 17.766229441914117 38.07375343378007 32.373737974337324 5 12.430466206397941 39.386628107467914 35.74078610346771 12.442119582666434 6 31.81230175115339 38.528954773605484 17.605332938725144 12.053410536515976 7 28.134299831017717 31.311381117569514 21.961266259632524 18.593052791780245 8 23.95592745897978 40.703731276614754 19.571305202346565 15.769036062058902 9 26.147816816262353 15.535000625383418 19.73078732794511 38.586395230409124 10 14.770247855270119 30.283407549211372 34.38535579032479 20.560988805193723 11 34.1372828985459 22.101460044059305 25.608371505779665 18.15288555161513 12 24.440930652812288 24.514997681690108 32.2600549261113 18.784016739386303 13 30.184733646631962 22.666279897807424 25.231556059094796 21.91743039646582 14 19.56402764029544 24.359081131500798 27.095389476890276 28.981501751313488 15 21.686903115894044 34.56729506062613 22.359205791195034 21.386596032284793 16 20.364392926140585 28.747570218457426 30.524713924803095 20.3633229305989 17 20.253452002482444 30.83345024289447 28.806357343783066 20.10674041084001 18 20.355357408233022 28.26131668895824 32.068004161093775 19.315321741714968 19 23.71863155842365 27.209165743307828 26.75061702562737 22.321585672641156 20 23.563385104096326 28.810168056836876 28.19802554616094 19.428421292905853 21 21.464983903104777 27.37649139993057 26.924732154320957 24.233792542643695 22 22.59625729798361 30.6834514840868 26.46580840437635 20.25448281355324 23 20.792788979940724 30.170110930539725 28.070502890661313 20.96659719885824 24 23.641897153318947 27.440008084122425 29.000838133281025 19.9172566292776 25 21.64649405521205 29.096708462272797 28.459253147747688 20.79754433476747 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 142.0 1 142.0 2 146.0 3 150.0 4 150.0 5 150.0 6 255.5 7 361.0 8 361.0 9 361.0 10 628.0 11 895.0 12 895.0 13 895.0 14 1675.0 15 2455.0 16 2455.0 17 2455.0 18 4784.5 19 7114.0 20 7114.0 21 7114.0 22 12772.5 23 18431.0 24 18431.0 25 18431.0 26 29078.0 27 39725.0 28 39725.0 29 39725.0 30 56239.0 31 72753.0 32 72753.0 33 72753.0 34 83340.5 35 93928.0 36 93928.0 37 93928.0 38 100136.0 39 106344.0 40 106344.0 41 106344.0 42 110041.0 43 113738.0 44 113738.0 45 113738.0 46 116585.5 47 119433.0 48 119433.0 49 119433.0 50 110194.5 51 100956.0 52 100956.0 53 100956.0 54 84737.5 55 68519.0 56 68519.0 57 68519.0 58 57140.5 59 45762.0 60 45762.0 61 45762.0 62 36522.0 63 27282.0 64 27282.0 65 27282.0 66 20610.5 67 13939.0 68 13939.0 69 13939.0 70 9920.0 71 5901.0 72 5901.0 73 5901.0 74 4075.0 75 2249.0 76 2249.0 77 2249.0 78 1506.5 79 764.0 80 764.0 81 764.0 82 500.5 83 237.0 84 237.0 85 237.0 86 150.5 87 64.0 88 64.0 89 64.0 90 39.0 91 14.0 92 14.0 93 14.0 94 7.5 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0852397352693968 2 0.032930832175206294 3 0.03340636765788075 4 0.029364316055147846 5 0.02365789026305434 6 0.3798339667862242 7 0.16929063183210744 8 0.19401847693117932 9 0.1987738317579239 10 0.0168815096349433 11 0.0021399096720350662 12 0.003209864508052599 13 0.0 14 0.07668009658125653 15 0.0021399096720350662 16 0.0038042838613956726 17 0.006063077404099354 18 0.0038042838613956726 19 0.0029720967667153696 20 0.0015454903186919921 21 1.1888387066861477E-4 22 0.0 23 0.0 24 2.3776774133722953E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 841157.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.6671089821211 #Duplication Level Percentage of deduplicated Percentage of total 1 92.2448835903845 72.56638310447853 2 5.720634034390174 9.000514820604058 3 0.9499317169249205 2.241851457027184 4 0.357372128713765 1.1245372878679345 5 0.18054300564781756 0.7101398150628286 6 0.10625034752648017 0.5015044600952314 7 0.06916646613662218 0.38087881506344917 8 0.05139659543194962 0.32345772593241345 9 0.033816455157274466 0.23942184869216895 >10 0.2325802996373221 3.3622202190186843 >50 0.026865871655049837 1.5138966236580726 >100 0.02155112783254611 3.4648721674965346 >500 0.0036424440447981456 2.027154947238638 >1k 0.0013659165167993045 2.5431667077643105 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTAGGACGTGGAATATGGCAAGA 3624 0.43083514730305994 No Hit GTCCTACAGTGGACATTTCTAAATT 3479 0.41359698605611084 No Hit CTTTAGGACGTGAAATATGGCGAGG 2770 0.3293083217520629 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2752 0.32716841208002784 No Hit GTCCTACAGTGTGCATTTCTCATTT 2204 0.26202005095362696 No Hit CTGTAGGACCTGGAATATGGCGAGA 1919 0.22813814781307176 No Hit CTGAAGGACCTGGAATATGGCGAGA 1667 0.1981794124045808 No Hit CCCATGTACTCTGCGTTGATACCAC 1563 0.1858154898550449 No Hit GTACATGGGAAGCAGTGGTATCAAC 1323 0.15728336089457734 No Hit GTCCTTCAGTGTGCATTTCTCATTT 995 0.1182894513152717 No Hit GAATATGGCAAGAAAACTGAAAATC 965 0.11472293519521326 No Hit GATATACACTGTTCTACAAATCCCG 884 0.10509334167105547 No Hit CTGTAGGACATGGAATATGGCAAGA 869 0.10331008361102623 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 1.1888387066861478E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 615 0.0 14.421046 4 ACGAATG 40 0.0053684674 14.212904 19 CCAACGA 110 1.9281288E-10 13.782209 19 CCTCTAA 65 5.268897E-5 13.200434 1 GGACTTG 155 0.0 12.919644 6 TGTAGGA 1990 0.0 12.897991 2 TAAGGTC 60 3.9583634E-4 12.715331 5 GACTTGG 135 2.7284841E-11 12.663806 7 CTGTAGG 2005 0.0 12.64813 1 GTAGGAC 1950 0.0 12.570872 3 TCTACAC 100 1.3659883E-7 12.399673 3 GTCCTAC 1735 0.0 12.363518 1 GGCGAGG 810 0.0 12.282757 19 TCCAACG 155 1.8189894E-12 12.226153 18 GTAGCAC 55 0.0029755828 12.139541 3 CCCGTTT 125 1.4515535E-9 12.135559 12 GTCTAAG 55 0.002986273 12.133732 1 TAGGACT 215 0.0 11.976068 4 TGTCGAG 80 2.9400768E-5 11.844087 18 TCCTACA 1855 0.0 11.727726 2 >>END_MODULE