FastQCFastQC Report
Thu 26 May 2016
SRR1926476_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926476_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences712339
Sequences flagged as poor quality0
Sequence length25
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACCG451.6215101E-518.4898975
GCTACAC603.425066E-718.1891923
CCCGCTG300.001059543618.12369212
GGATAGT500.002483610515.9558861
GTCTAGC550.00468419314.5053521
GTCTTAG658.5661095E-414.3193851
TAGGACC1600.014.037994
GTCCTAG1101.6292888E-613.2965731
GTATAAG700.001499141713.2965731
GTATTAA600.008336144513.2965721
TGTAGGA2700.013.1200982
CCTAGGG500.0092191913.0962173
GACCGTG550.001825517612.9544397
TGTACTG1203.6085112E-812.1339955
GTAGGAC3250.012.0888173
GTGTATG1554.154026E-812.0098071
CTAGGAC1005.1247007E-612.0048663
GTCCTAA1351.2413911E-611.8191761
TAGGACA1951.8189894E-1211.5183514
AGCTACA959.373565E-411.4734452
Produced by FastQC (version 0.11.5)