##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926476_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 712339 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.629345016909085 27.0 10.0 31.0 2.0 31.0 2 16.638590614861744 22.0 2.0 30.0 2.0 31.0 3 20.222677685764783 25.0 15.0 31.0 2.0 31.0 4 22.372406957923122 28.0 7.0 35.0 2.0 37.0 5 22.948530123999948 29.0 7.0 35.0 2.0 37.0 6 23.80432209944984 31.0 8.0 35.0 2.0 37.0 7 23.56763703798332 30.0 10.0 35.0 2.0 37.0 8 23.983669292289203 31.0 12.0 35.0 2.0 37.0 9 26.841032149018936 32.0 17.0 37.0 2.0 39.0 10 27.606542671396625 32.0 25.0 37.0 2.0 39.0 11 24.65616932387529 30.0 10.0 37.0 2.0 39.0 12 25.823843703629873 31.0 17.0 37.0 2.0 39.0 13 22.417412215251446 28.0 2.0 37.0 2.0 39.0 14 25.853675005860975 31.0 17.0 38.0 2.0 40.0 15 26.978644999080494 31.0 18.0 38.0 2.0 40.0 16 27.339365105658963 32.0 21.0 39.0 2.0 40.0 17 26.621077885669603 31.0 17.0 38.0 2.0 40.0 18 26.699678102701103 31.0 17.0 38.0 2.0 40.0 19 27.127235768363096 31.0 21.0 39.0 2.0 40.0 20 27.40069685922012 32.0 24.0 39.0 2.0 40.0 21 25.18972146688585 30.0 10.0 38.0 2.0 40.0 22 26.157761964457933 31.0 16.0 38.0 2.0 40.0 23 26.146594528728595 31.0 10.0 38.0 2.0 40.0 24 25.20799647358912 31.0 2.0 39.0 2.0 40.0 25 25.009093984746027 31.0 2.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.537823946127535 1101 2 10.455636786369208 1101 3 6.839344051991322 1101 4 8.473026340454034 1101 5 8.165787971429634 1101 6 7.618409833906178 1101 7 7.305087655624483 1101 8 5.580284728291126 1101 9 5.760378568475922 1101 10 5.2597637589642545 1101 11 8.304204520094277 1101 12 5.831004584568937 1101 13 10.403696846786175 1101 14 7.627805313815131 1101 15 6.832200615041511 1101 16 6.2843987214790715 1101 17 2.4877100021522267 1101 18 4.886170325427855 1101 19 5.292999344869763 1101 20 3.228839585886199 1101 21 0.6339328678558012 1101 22 4.454024798005822 1101 23 5.380816384608128 1101 24 6.953320966640344 1101 25 7.499031648194226 1102 1 6.108559596895521 1102 2 10.99888658426735 1102 3 7.224954399606521 1102 4 8.747884869152497 1102 5 8.54735628185809 1102 6 7.337084045708927 1102 7 7.273559765567892 1102 8 7.621513507595896 1102 9 6.721575011483193 1102 10 5.946910080710417 1102 11 8.974374285656122 1102 12 5.862532474625528 1102 13 10.433607921968068 1102 14 8.263213559570993 1102 15 5.998732547135287 1102 16 6.403234614769289 1102 17 7.193449694957401 1102 18 7.136776631005869 1102 19 5.964785925306302 1102 20 6.30159625524756 1102 21 5.513480159690886 1102 22 6.42492213026128 1102 23 6.945084775877326 1102 24 8.435940206737357 1102 25 8.179710710441597 1103 1 4.554631921372824 1103 2 8.570424784447777 1103 3 5.734048657830861 1103 4 7.141154909781154 1103 5 6.589265242943881 1103 6 6.184101074336443 1103 7 6.156927791426515 1103 8 6.048498434246916 1103 9 4.792613977763104 1103 10 4.3752156665324335 1103 11 5.199177944187941 1103 12 3.7807629084968895 1103 13 8.023365230505032 1103 14 6.196757521444361 1103 15 5.232183952215102 1103 16 4.982241796707825 1103 17 2.6665555026032948 1103 18 1.1717425307919846 1103 19 3.464118110466753 1103 20 3.964287275676444 1103 21 6.259053120089337 1103 22 1.8729855323157558 1103 23 4.094045236812764 1103 24 5.484063881359219 1103 25 1.026008503788706 1104 1 5.29637766213278 1104 2 9.975339868671774 1104 3 6.676171802039718 1104 4 7.316513001088193 1104 5 7.1107641726092545 1104 6 6.768253972695007 1104 7 6.77839059489617 1104 8 6.819811928918302 1104 9 5.4740981494932015 1104 10 4.454326270339521 1104 11 7.484179039503449 1104 12 4.708012821063807 1104 13 9.157485653719572 1104 14 6.672797014294066 1104 15 5.965945419399834 1104 16 5.347154805597178 1104 17 5.767347588660506 1104 18 3.1500706565726375 1104 19 4.188876852813955 1104 20 3.476814226202773 1104 21 2.659865575171793 1104 22 4.768580826686602 1104 23 5.136043860942756 1104 24 6.211658914391627 1104 25 5.174466030527853 1105 1 3.7301124970572026 1105 2 10.064288009085455 1105 3 6.6800618925329545 1105 4 6.057256711310945 1105 5 2.1466047155686816 1105 6 5.194707706784108 1105 7 6.224052388850026 1105 8 3.3041005377090755 1105 9 4.387966735412057 1105 10 4.041923848899586 1105 11 7.050759314960889 1105 12 4.268159748271444 1105 13 5.389479612550119 1105 14 4.370377438916179 1105 15 4.666186568962285 1105 16 4.853618608302028 1105 17 5.503557314197497 1105 18 3.24808738787733 1105 19 3.299681969162407 1105 20 2.3218582297827233 1105 21 3.1114597726658246 1105 22 4.737933700592727 1105 23 2.0929570222394958 1105 24 5.178923482808493 1105 25 6.13367632786278 1106 1 6.674049118837061 1106 2 11.393643737032374 1106 3 7.944389688200403 1106 4 9.211182477314068 1106 5 8.85746415155619 1106 6 7.822140498548354 1106 7 7.803192221012399 1106 8 7.571376469598224 1106 9 6.463204586700112 1106 10 5.957533602022146 1106 11 8.220911417810225 1106 12 5.616702146788228 1106 13 4.6774888582565985 1106 14 6.127118388369734 1106 15 6.328108407369779 1106 16 6.3316631047891505 1106 17 6.5920892228626045 1106 18 6.3242577381895835 1106 19 6.232624372558192 1106 20 5.487191709486083 1106 21 6.255255513298145 1106 22 6.520592512068134 1106 23 6.8495955126766574 1106 24 7.675575268522778 1106 25 6.842081016582529 1107 1 4.039728337983551 1107 2 -14.031024490914543 1107 3 -5.5816355021139685 1107 4 1.8405669096784472 1107 5 4.497101820731931 1107 6 5.07506612079322 1107 7 4.79238240023955 1107 8 5.35351385870376 1107 9 3.4130604616838873 1107 10 2.764137568527527 1107 11 5.581282639752082 1107 12 3.057730031111234 1107 13 4.608746293453688 1107 14 3.8329824360688 1107 15 3.679177742083013 1107 16 3.263546474664974 1107 17 3.920525328624226 1107 18 -1.4449679699434732 1107 19 1.5492424048116078 1107 20 2.038874886958123 1107 21 -1.365011967083607 1107 22 1.7133928406838415 1107 23 -1.0904943587400027 1107 24 2.556907300196496 1107 25 3.355587513322156 1108 1 5.107519591651798 1108 2 10.46318400329395 1108 3 -0.05271139414658066 1108 4 5.724193849341834 1108 5 7.22858468661115 1108 6 7.099820520490674 1108 7 7.222640709313346 1108 8 7.204260407400195 1108 9 5.498928366686187 1108 10 4.547284008165995 1108 11 6.851896501254323 1108 12 2.0413197386189346 1108 13 2.8891689223956796 1108 14 4.553217086599577 1108 15 4.740185121085837 1108 16 4.548309727993146 1108 17 0.23494003620522008 1108 18 3.4640049795761705 1108 19 4.599672919934605 1108 20 4.696285112022107 1108 21 4.848938657800961 1108 22 1.5030217797433068 1108 23 4.206322731698954 1108 24 4.981366774314285 1108 25 5.718229296009497 1109 1 3.259625080599392 1109 2 -14.031024490914543 1109 3 -5.745271623548916 1109 4 1.5511170131071026 1109 5 0.5069156491526385 1109 6 2.207626070401485 1109 7 3.699504038808257 1109 8 4.078052996982258 1109 9 3.371664260474713 1109 10 2.3665890274710115 1109 11 0.7014844350527838 1109 12 1.5613314796833073 1109 13 2.754553103569336 1109 14 3.3052412912553635 1109 15 3.152806765286428 1109 16 2.983839995102361 1109 17 3.597142840513289 1109 18 3.7228909331989684 1109 19 2.1106801677839577 1109 20 1.2376881692146355 1109 21 0.4533189779707527 1109 22 2.721532019306178 1109 23 3.0937304401592947 1109 24 4.241991308623028 1109 25 4.637676562825185 1110 1 3.0235807347621204 1110 2 9.094385345151032 1110 3 6.17577142122148 1110 4 7.315671158032256 1110 5 8.146886477863763 1110 6 3.731195923997223 1110 7 6.459554438030356 1110 8 7.4206348614795665 1110 9 5.513555874756317 1110 10 5.244574975948765 1110 11 3.0967506059444965 1110 12 5.72966077286161 1110 13 10.170306969107497 1110 14 7.5907795905555275 1110 15 6.8692091099458885 1110 16 6.497471067318422 1110 17 7.093260183049956 1110 18 6.98132253131995 1110 19 5.641523669982078 1110 20 5.8951932707663275 1110 21 3.6785576210264814 1110 22 6.001740053051744 1110 23 6.55587966568212 1110 24 7.466090491005211 1110 25 7.82877110245294 1111 1 4.935458019590225 1111 2 9.48335002345997 1111 3 7.433152291717104 1111 4 8.059006784154768 1111 5 8.50657096459743 1111 6 7.6222056428757945 1111 7 7.469698756371393 1111 8 7.626294229434016 1111 9 6.093217560975379 1111 10 5.352936013818002 1111 11 7.260358309716132 1111 12 6.485237982537178 1111 13 8.205780239006998 1111 14 6.014274947657441 1111 15 5.410128391029108 1111 16 5.342837722521139 1111 17 6.753647154912336 1111 18 6.415157730728925 1111 19 5.456398776660464 1111 20 4.84908846482546 1111 21 7.75669396555627 1111 22 7.162255038976568 1111 23 6.973902899279125 1111 24 6.898019490967002 1111 25 7.359212336992538 1112 1 -2.24807823918875 1112 2 7.94632178740261 1112 3 6.136718951594446 1112 4 -0.06238072800944394 1112 5 3.8513933482547635 1112 6 2.979803277172458 1112 7 5.949264767490803 1112 8 6.10366313884176 1112 9 4.4585927467389155 1112 10 3.395137339999909 1112 11 -0.49653285736920694 1112 12 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0.4942876953626083 2207 11 -1.2842361633349313 2207 12 -2.498223893207655 2207 13 0.42900181914176017 2207 14 -5.574852163173851 2207 15 -1.634607241327103 2207 16 -0.3115743177429877 2207 17 0.7079483913679177 2207 18 0.9509235012213821 2207 19 0.23652129794054133 2207 20 0.04330617994349595 2207 21 -3.7874826509676147 2207 22 -4.311980889439429 2207 23 -1.3356126120048764 2207 24 0.27651693136154876 2207 25 1.0558703592132623 2208 1 5.3163552729707 2208 2 6.769124651516478 2208 3 6.8559293655008915 2208 4 8.678370269290067 2208 5 3.9641044825336316 2208 6 6.642303367671506 2208 7 7.208068514875382 2208 8 7.33184743834293 2208 9 5.865342527731219 2208 10 5.189322595171021 2208 11 7.2982279717468685 2208 12 6.7532093847367705 2208 13 9.557511282013209 2208 14 4.160455389139894 2208 15 5.806555230408964 2208 16 6.080967601590622 2208 17 6.838361098686683 2208 18 1.2249907016357184 2208 19 4.234098332883509 2208 20 5.429481971393464 2208 21 7.991655871659116 2208 22 7.091209008198987 2208 23 3.2566321014714106 2208 24 4.393589543584035 2208 25 5.764139251986567 2209 1 2.738167715503806 2209 2 4.786127612645327 2209 3 5.005650990429395 2209 4 0.582441278463051 2209 5 4.8700017624942475 2209 6 5.482230461638476 2209 7 6.095704896940642 2209 8 4.727449546608749 2209 9 4.471537730557689 2209 10 3.4655256895144717 2209 11 -0.4188629544565856 2209 12 3.0147262935788888 2209 13 2.353595207210315 2209 14 3.6034301290456305 2209 15 4.0168641579590485 2209 16 3.5570975727033236 2209 17 1.3676248708318006 2209 18 3.4104938401427027 2209 19 3.9320722401980035 2209 20 4.128608836578842 2209 21 6.152800286419872 2209 22 5.034227187647744 2209 23 0.6089991945695914 2209 24 -2.8868380463177203 2209 25 0.3185291798045249 2210 1 -1.7107592134691139 2210 2 -14.031024490914543 2210 3 -7.469540629396869 2210 4 -2.0515563369346665 2210 5 -0.19684374934359994 2210 6 -0.6665176221632585 2210 7 -0.6601033129043579 2210 8 -21.315430712290926 2210 9 -12.957784361079172 2210 10 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-5.736040693680401 2211 23 -8.073401044941122 2211 24 -22.610139017191507 2211 25 -22.39991742213722 2212 1 -0.9059939218617146 2212 2 -14.031024490914543 2212 3 -7.784500325935513 2212 4 -19.763837038689054 2212 5 -20.24245778443132 2212 6 -21.06388604321589 2212 7 -9.851876665204028 2212 8 -9.697412694272908 2212 9 -4.952165584407762 2212 10 -5.399820238938254 2212 11 -5.91412666476884 2212 12 -2.988667391368196 2212 13 -6.105811772585016 2212 14 -3.782304148921657 2212 15 -3.705632124731409 2212 16 -3.874984995301574 2212 17 -3.8267073254421433 2212 18 -3.0092253936542015 2212 19 -3.6515497312880463 2212 20 -3.8769452612082667 2212 21 -1.6294397205774196 2212 22 -3.007660330938805 2212 23 -3.190974565598342 2212 24 -2.2137426207951094 2212 25 -2.698115620335418 2213 1 -19.087075002942797 2213 2 -14.031024490914543 2213 3 -8.850374336180383 2213 4 -6.304991248717437 2213 5 -20.24245778443132 2213 6 -11.058209600453356 2213 7 -20.897822611149973 2213 8 -11.131892396302279 2213 9 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-12.02429657947922 2214 22 -13.037722262124332 2214 23 -13.059674010702281 2214 24 -11.859372929346767 2214 25 -11.579692703036098 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 117941.0 3 2.0 4 462.0 5 1785.0 6 2592.0 7 2588.0 8 2370.0 9 2257.0 10 2148.0 11 1974.0 12 1905.0 13 2191.0 14 3441.0 15 7173.0 16 10632.0 17 11378.0 18 11520.0 19 13744.0 20 16035.0 21 18454.0 22 19334.0 23 21369.0 24 22710.0 25 23026.0 26 19130.0 27 15057.0 28 16522.0 29 18944.0 30 18542.0 31 21549.0 32 28044.0 33 35325.0 34 42870.0 35 48807.0 36 48035.0 37 49635.0 38 32848.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.06709225913277 18.167720945876024 11.71640536065404 26.048781434337165 2 14.39254927327692 20.588507082414754 40.13324356554149 24.88570007876684 3 19.51759702871184 23.704263417725098 28.52754941610975 28.25059013745331 4 11.496045359098272 16.931283043239787 37.99067892599937 33.58199267166257 5 12.730601894237079 37.12024111418085 35.98985635233782 14.159300639244249 6 31.976342685766152 37.95364560725044 17.587440642963315 12.4825710640201 7 28.14538791791611 30.804941842052003 21.794283542590712 19.255386697441175 8 25.02461078499289 36.98925025218458 20.7236482298465 17.262490732976023 9 26.111654524195 15.455089719373987 19.780985788810938 38.65226996762007 10 13.545537015068634 29.778962014392178 35.24260631427241 21.432894656266775 11 33.83771655745802 22.28347229909479 24.166949359329156 19.711861784118028 12 22.762007108708467 24.732976905628025 32.132374391979184 20.372641593684325 13 27.98399978612914 21.083991097625365 25.441275739692426 25.49073337655307 14 20.35173729647407 21.73952103493416 27.99752446303949 29.911217205552276 15 22.70276131270782 30.621078502240856 23.491831718953303 23.18432846609802 16 22.143029734801626 28.45619502765708 27.11378108437762 22.286994153163672 17 21.573096389477428 28.09941004910958 28.452195478681126 21.87529808273187 18 21.389326638378392 27.871169612807805 29.650253198104366 21.08925055070944 19 22.662580059846384 27.221273523032025 27.311427639571534 22.804718777550057 20 22.707511495175265 27.684155101098412 28.260532903091377 21.347800500634946 21 22.143325755162966 26.335780950270276 26.95646686030384 24.564426434262916 22 22.17111643562097 27.982486165782415 27.974784642592276 21.871612756004335 23 22.27330093403304 27.55299084518325 27.747499079458866 22.426209141324847 24 22.094956358285764 27.44813453819902 28.312032478988037 22.144876624527182 25 22.557664307438035 27.264550294294565 27.868880106586914 22.308905291680485 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 612.0 1 612.0 2 1344.5 3 2077.0 4 2077.0 5 2077.0 6 3010.5 7 3944.0 8 3944.0 9 3944.0 10 4655.0 11 5366.0 12 5366.0 13 5366.0 14 6438.0 15 7510.0 16 7510.0 17 7510.0 18 10534.0 19 13558.0 20 13558.0 21 13558.0 22 19375.5 23 25193.0 24 25193.0 25 25193.0 26 33602.5 27 42012.0 28 42012.0 29 42012.0 30 51323.0 31 60634.0 32 60634.0 33 60634.0 34 68453.5 35 76273.0 36 76273.0 37 76273.0 38 81498.5 39 86724.0 40 86724.0 41 86724.0 42 88761.5 43 90799.0 44 90799.0 45 90799.0 46 89554.0 47 88309.0 48 88309.0 49 88309.0 50 82161.5 51 76014.0 52 76014.0 53 76014.0 54 65962.0 55 55910.0 56 55910.0 57 55910.0 58 46622.0 59 37334.0 60 37334.0 61 37334.0 62 29534.0 63 21734.0 64 21734.0 65 21734.0 66 16307.5 67 10881.0 68 10881.0 69 10881.0 70 7802.5 71 4724.0 72 4724.0 73 4724.0 74 3295.5 75 1867.0 76 1867.0 77 1867.0 78 1254.0 79 641.0 80 641.0 81 641.0 82 406.0 83 171.0 84 171.0 85 171.0 86 104.0 87 37.0 88 37.0 89 37.0 90 26.0 91 15.0 92 15.0 93 15.0 94 7.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.18290869936926 2 36.19526657953587 3 5.620638488135564 4 13.490908120992954 5 13.364984929928026 6 14.621549571201353 7 9.13188804768516 8 7.593435148152776 9 0.11048110520412331 10 1.4251641423535704 11 5.64773232969134 12 5.683670274967397 13 15.98213771813701 14 1.5550180461830674 15 2.897356455283229 16 2.9755495627783963 17 2.8661915183641495 18 1.476403790891696 19 1.4330255678827075 20 0.28652088401730075 21 4.35129903037739 22 1.5693370712539956 23 0.1124464615864076 24 8.886780030294565 25 0.11328875717881515 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 712339.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 95.20160859984806 #Duplication Level Percentage of deduplicated Percentage of total 1 97.35512811306899 92.68364801808457 2 2.0993814469512295 3.9972898162886725 3 0.28151520990408807 0.8040210248457919 4 0.09538459937318479 0.36323069183916995 5 0.05066637266026409 0.24117600895882516 6 0.03018924992431764 0.17244390931307282 7 0.015496142646823745 0.10326803949492294 8 0.012771656633221863 0.09727058047741129 9 0.008312362432279327 0.07122152473361437 >10 0.043969540976611564 0.7242539509230024 >50 0.004940027458175372 0.31238824440293506 >100 0.002245277970784999 0.42978819063810525 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.4038259873459124E-4 0.0 5 0.0 0.0 0.0 1.4038259873459124E-4 0.0 6 0.0 0.0 0.0 1.4038259873459124E-4 0.0 7 0.0 0.0 0.0 1.4038259873459124E-4 0.0 8 0.0 0.0 0.0 1.4038259873459124E-4 0.0 9 0.0 0.0 0.0 1.4038259873459124E-4 0.0 10 0.0 0.0 0.0 2.807651974691825E-4 0.0 11 0.0 0.0 0.0 2.807651974691825E-4 0.0 12 0.0 0.0 0.0 2.807651974691825E-4 0.0 13 0.0 0.0 0.0 2.807651974691825E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGACCG 45 1.6215101E-5 18.489897 5 GCTACAC 60 3.425066E-7 18.189192 3 CCCGCTG 30 0.0010595436 18.123692 12 GGATAGT 50 0.0024836105 15.955886 1 GTCTAGC 55 0.004684193 14.505352 1 GTCTTAG 65 8.5661095E-4 14.319385 1 TAGGACC 160 0.0 14.03799 4 GTCCTAG 110 1.6292888E-6 13.296573 1 GTATAAG 70 0.0014991417 13.296573 1 GTATTAA 60 0.0083361445 13.296572 1 TGTAGGA 270 0.0 13.120098 2 CCTAGGG 50 0.00921919 13.096217 3 GACCGTG 55 0.0018255176 12.954439 7 TGTACTG 120 3.6085112E-8 12.133995 5 GTAGGAC 325 0.0 12.088817 3 GTGTATG 155 4.154026E-8 12.009807 1 CTAGGAC 100 5.1247007E-6 12.004866 3 GTCCTAA 135 1.2413911E-6 11.819176 1 TAGGACA 195 1.8189894E-12 11.518351 4 AGCTACA 95 9.373565E-4 11.473445 2 >>END_MODULE