FastQCFastQC Report
Thu 26 May 2016
SRR1926476_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926476_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences712339
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT7450.10458503605727049No Hit
CTGTAGGACGTGGAATATGGCAAGA7310.10261967967498621No Hit
GTATCAACGCAGAGTACTTTTTTTT7140.10023317549649816No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGTT350.002202300816.24820911
CCGGTTT400.0053566914.21718212
TAGGACT1154.978574E-912.444054
CTCCTAG701.0496981E-412.262811
TGGACCG550.002969605112.1423765
CGGTTTC550.003119027512.06306513
AGGACTG1700.011.7852485
CTTAGAC907.089373E-611.6621843
AGTACTG1156.64495E-811.6144475
TCCTACA5400.011.4862972
TGGCGAG3550.011.474918
GGCGAGA2600.011.29449119
GTCCTAC4650.011.2811991
TAGACTG1104.6882997E-711.27506355
GTAGGAC6000.011.1320863
GGATAAT600.005687581411.130516
TGTAGGA6150.011.01652
GACGTGG2350.010.9158237
AGTAGTG700.001437794310.9033585
CCTACAG5000.010.8776393
Produced by FastQC (version 0.11.5)