##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926476_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 712339 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.718539908667083 31.0 28.0 31.0 23.0 34.0 2 29.17380629166731 31.0 28.0 33.0 25.0 34.0 3 29.52484561423704 31.0 30.0 33.0 25.0 34.0 4 33.33795847201964 35.0 33.0 37.0 28.0 37.0 5 33.03755374898749 35.0 33.0 37.0 27.0 37.0 6 33.15416255462638 35.0 33.0 37.0 28.0 37.0 7 32.99590503959491 35.0 33.0 37.0 26.0 37.0 8 32.9747170939679 35.0 33.0 37.0 26.0 37.0 9 34.26407651413162 38.0 34.0 39.0 26.0 39.0 10 34.16514187767341 37.0 34.0 39.0 26.0 39.0 11 34.421296601758435 38.0 35.0 39.0 27.0 39.0 12 34.22129632099323 38.0 34.0 39.0 27.0 39.0 13 34.381335291202646 38.0 35.0 39.0 27.0 39.0 14 35.0449406813329 39.0 34.0 40.0 25.0 41.0 15 35.194088769532485 39.0 34.0 40.0 27.0 41.0 16 35.16330567328196 39.0 34.0 40.0 27.0 41.0 17 35.0879300445434 39.0 34.0 40.0 26.0 41.0 18 35.05757932669698 39.0 34.0 40.0 26.0 41.0 19 35.10865191994261 39.0 34.0 41.0 26.0 41.0 20 35.09421216583677 39.0 34.0 41.0 26.0 41.0 21 35.022462619623525 39.0 34.0 41.0 25.0 41.0 22 34.962863187330754 39.0 34.0 41.0 25.0 41.0 23 34.63012975563601 39.0 34.0 40.0 23.0 41.0 24 34.706785673675036 39.0 34.0 41.0 24.0 41.0 25 34.51538803856029 39.0 34.0 40.0 23.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7150410504766036 1101 2 0.8120708467222855 1101 3 0.845741706626459 1101 4 0.5751495386275636 1101 5 0.6226871747060798 1101 6 0.020003735859873473 1101 7 0.3183413494739824 1101 8 0.6638715603161103 1101 9 0.7955607161509448 1101 10 0.7201279298016985 1101 11 0.6090641910187244 1101 12 0.6077024689183972 1101 13 0.4209174236624662 1101 14 0.8176616179874685 1101 15 0.6182635830769172 1101 16 0.5134173635392045 1101 17 0.6565511745850756 1101 18 0.5850266431237969 1101 19 0.5468101813403621 1101 20 0.6028220245994902 1101 21 0.5585680284748875 1101 22 0.5479191759849726 1101 23 0.7946130987828184 1101 24 0.4487868083745852 1101 25 0.43591297134585005 1102 1 0.7465689405331908 1102 2 0.8080288095355428 1102 3 0.6274716985423865 1102 4 0.34798704873265507 1102 5 0.5766079507772233 1102 6 0.31103041330530345 1102 7 0.5980503227965386 1102 8 0.5636290380849118 1102 9 0.7680748632811003 1102 10 0.8680664908364619 1102 11 0.5266066324091838 1102 12 0.7208795101471779 1102 13 0.6965843597982797 1102 14 0.8588903972922424 1102 15 0.7217397350575183 1102 16 0.7656404839919091 1102 17 0.7406255480693105 1102 18 0.7523669826549195 1102 19 0.6907067051883757 1102 20 0.5502755411718425 1102 21 0.7315672200674541 1102 22 0.7653807766317016 1102 23 1.0799809241668115 1102 24 1.0267981260787096 1102 25 1.0559614757920883 1103 1 0.4050630115610936 1103 2 0.34449501458984955 1103 3 0.21485585609982394 1103 4 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0.011740297901702945 2204 8 0.11660671498668762 2204 9 0.4610522574330602 2204 10 0.25989219356615223 2204 11 0.08629511922212174 2204 12 0.2071404393916012 2204 13 -0.08349475874201318 2204 14 0.20906062553560645 2204 15 0.12432021272477556 2204 16 0.16859753300916225 2204 17 0.06666757754469188 2204 18 0.06849349006489547 2204 19 0.1970197375131093 2204 20 0.12041484686680803 2204 21 0.18094472063616251 2204 22 0.24128727897787883 2204 23 0.37823185949036287 2204 24 0.24738925313234006 2204 25 0.1989249252370655 2205 1 0.4270916981716404 2205 2 0.43622641107258175 2205 3 0.3821109756398222 2205 4 0.006048834068906217 2205 5 0.15268046611226538 2205 6 0.15822140065650814 2205 7 0.1542183840129212 2205 8 0.13211765379502083 2205 9 0.47937274797956775 2205 10 0.5157421475190134 2205 11 0.45202682608935163 2205 12 0.4735812353124018 2205 13 0.27593051886260156 2205 14 0.702951541154377 2205 15 0.6112911470820919 2205 16 0.614663361359689 2205 17 0.5764819503998737 2205 18 0.7006776737026073 2205 19 0.601646214679505 2205 20 0.734828769455973 2205 21 0.6399289539482282 2205 22 0.5809319598263301 2205 23 0.7596915230572989 2205 24 0.7027530378259996 2205 25 0.6583806960071499 2206 1 0.37448991427135425 2206 2 0.5088673738647564 2206 3 0.5728813887880264 2206 4 0.38234499836811153 2206 5 0.5279571549140414 2206 6 0.14244135037370143 2206 7 0.4777509332835308 2206 8 0.5805789852469943 2206 9 0.7949392054173714 2206 10 0.8427549752118964 2206 11 0.6354451359177773 2206 12 0.5281243866432987 2206 13 0.41630962797921 2206 14 0.6210583230302618 2206 15 0.5002665075351729 2206 16 0.5966928328083156 2206 17 0.6603626002458043 2206 18 0.6289828472073964 2206 19 0.4548405308308574 2206 20 0.47986109931508736 2206 21 0.4381412957840567 2206 22 0.47249843665962743 2206 23 0.8756141301992031 2206 24 0.550258414013669 2206 25 0.5333488752796782 2207 1 -0.9053687679046583 2207 2 0.043886878143602814 2207 3 0.2378765001186487 2207 4 0.14821553430738987 2207 5 0.26781190599508875 2207 6 0.4894285188765579 2207 7 0.6648836530972559 2207 8 0.7492254790126793 2207 9 0.948612259273375 2207 10 0.5974118107812529 2207 11 0.5038542520261942 2207 12 0.5608356325988098 2207 13 0.10887280269118094 2207 14 -5.243061867678243 2207 15 -1.9252468027491787 2207 16 -0.586470993054256 2207 17 -0.27165717618576934 2207 18 0.23700984072613096 2207 19 0.39808660729569567 2207 20 0.2713996694044951 2207 21 0.28336703135240526 2207 22 0.2545796994482856 2207 23 0.5572584366633322 2207 24 0.15822996901371766 2207 25 0.2879679118590204 2208 1 0.4798071458325097 2208 2 0.8580313113184417 2208 3 0.8386927072491943 2208 4 0.7931090844966988 2208 5 0.6545032219252747 2208 6 0.7244141681137037 2208 7 0.8408998957150686 2208 8 1.0339310665787096 2208 9 1.0534915186445275 2208 10 1.248782386562148 2208 11 0.8363315749435358 2208 12 0.9054073996009251 2208 13 0.8131529746605537 2208 14 1.3036356585235893 2208 15 0.9343177349212723 2208 16 1.0456261605312775 2208 17 0.775344869127629 2208 18 1.1365317217430757 2208 19 0.6701444611313221 2208 20 0.7588784190058604 2208 21 0.9479997741104853 2208 22 0.8765557887035555 2208 23 1.4025819102912607 2208 24 1.2230403878833513 2208 25 1.107955155625369 2209 1 -0.16813164785138213 2209 2 -0.17664611387504436 2209 3 0.5231786364572173 2209 4 0.5216481402658317 2209 5 0.7138181079326742 2209 6 0.5710943010387055 2209 7 0.6955837644407694 2209 8 0.9029861841188733 2209 9 1.1337026348235852 2209 10 0.7175457351717256 2209 11 0.8194064429959198 2209 12 0.6867942794109467 2209 13 0.8867268906629562 2209 14 1.1411174682149507 2209 15 1.1762995192729022 2209 16 1.1255603496236048 2209 17 1.128776231029704 2209 18 1.2982643803155085 2209 19 1.2471120914318519 2209 20 1.2028323586104221 2209 21 1.1212529614869524 2209 22 1.2683207373362961 2209 23 1.1848430109044799 2209 24 1.2894540192119521 2209 25 1.5353169997787717 2210 1 -0.5791178512238879 2210 2 -0.9109588374315081 2210 3 -0.06630356419003292 2210 4 0.06428142250054236 2210 5 0.2181870399715109 2210 6 0.337854648509321 2210 7 0.4204006610512252 2210 8 0.5392491716848724 2210 9 0.8433773069407735 2210 10 0.8003169836813484 2210 11 0.5155144314135711 2210 12 0.4072422439825729 2210 13 0.4111179654372705 2210 14 0.7060552982268717 2210 15 0.40953065536556466 2210 16 0.41258130005794413 2210 17 0.47234632130393095 2210 18 0.5065168074999775 2210 19 0.41049141352348784 2210 20 0.542680766036753 2210 21 0.8142013518804134 2210 22 0.6605367141715845 2210 23 1.1914943479850493 2210 24 0.8920089502288135 2210 25 0.8304285650998366 2211 1 -0.9689663712022494 2211 2 -0.27649713250841046 2211 3 0.5248858631227442 2211 4 0.5213801163717875 2211 5 0.730228155977386 2211 6 0.5178801527986749 2211 7 0.4890071994235896 2211 8 0.5411040290927147 2211 9 0.8213122323600501 2211 10 0.6923372325413837 2211 11 0.441899778040046 2211 12 0.5330440833828334 2211 13 0.47248669563281 2211 14 0.8526624548850421 2211 15 0.581000629861272 2211 16 0.8104134354625359 2211 17 0.7141347129879918 2211 18 0.631994046853869 2211 19 0.7806977664101069 2211 20 0.5812386144021602 2211 21 0.7284489753443566 2211 22 0.9139682003760825 2211 23 1.0713227736748223 2211 24 0.8942463719770188 2211 25 1.201608718125577 2212 1 1.1390187063863522 2212 2 0.2887566675471547 2212 3 0.43189463400816663 2212 4 0.5614364722871699 2212 5 0.5587275805120484 2212 6 0.8486417514016864 2212 7 0.8890587928672531 2212 8 0.9371154590248452 2212 9 1.233230317846413 2212 10 0.9504592311920135 2212 11 0.8476623688246647 2212 12 1.103377853276072 2212 13 0.9452810759161707 2212 14 0.895600106192731 2212 15 0.9984590574518606 2212 16 0.2614433199725994 2212 17 0.9818769045187281 2212 18 0.9835438345270049 2212 19 0.6943700289810479 2212 20 0.9450041420443398 2212 21 1.109720555294352 2212 22 1.4126704268316175 2212 23 1.3848561308205163 2212 24 1.3246547539272484 2212 25 0.8446059003298032 2213 1 0.4167800060019573 2213 2 0.24655581011450778 2213 3 -0.42089202208974896 2213 4 -1.0397900143697782 2213 5 -2.180709207269107 2213 6 -0.635976452421815 2213 7 -0.5029524236472724 2213 8 -0.35673250435954174 2213 9 -0.7760437622885945 2213 10 -0.6549777100065128 2213 11 -0.6142619964109599 2213 12 -1.0721972487890952 2213 13 -1.0792810453346888 2213 14 -7.938764532807667 2213 15 -3.6486852486328445 2213 16 -2.4548214784114037 2213 17 -1.5755864244677795 2213 18 -5.857611227820112 2213 19 -3.580749934880636 2213 20 -5.299449393799925 2213 21 -2.3958842926946033 2213 22 -2.1900757441264744 2213 23 -4.571185530519159 2213 24 -2.596964310738173 2213 25 -2.0338994735398046 2214 1 0.08258402860336034 2214 2 -0.14193745865259189 2214 3 -1.886119703107294 2214 4 -2.2930362844327767 2214 5 -0.9068725446386381 2214 6 -0.831714191875605 2214 7 -1.0033346219980714 2214 8 -1.1484020298634299 2214 9 -6.173387024725983 2214 10 -2.046400038700959 2214 11 -1.4863188033587562 2214 12 -1.575720141552253 2214 13 -1.7955797031468492 2214 14 -3.0636569036260717 2214 15 -2.4205886930565654 2214 16 -1.9533318228924372 2214 17 -2.3016676205988347 2214 18 -1.6193088649549097 2214 19 -4.992642826571132 2214 20 -2.69534407133758 2214 21 -4.402133791391456 2214 22 -6.443487263033941 2214 23 -7.056626720774698 2214 24 -6.500461884672536 2214 25 -4.229304782569642 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 18901.0 3 22.0 4 353.0 5 2181.0 6 2841.0 7 2318.0 8 1657.0 9 1417.0 10 1309.0 11 1204.0 12 1178.0 13 1125.0 14 1165.0 15 1207.0 16 1199.0 17 1283.0 18 1385.0 19 1660.0 20 2316.0 21 2891.0 22 3843.0 23 4861.0 24 5867.0 25 6893.0 26 8054.0 27 8978.0 28 10499.0 29 12753.0 30 15593.0 31 20193.0 32 25930.0 33 33980.0 34 45602.0 35 60324.0 36 82023.0 37 121543.0 38 197791.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.73825682530315 18.860318397054897 8.817603169919487 30.583821607722463 2 13.826248260502632 21.143581728642623 38.54234684348807 26.48782316736668 3 18.39609062888357 23.921166632731154 28.83877354715047 28.84396919123481 4 12.162146981509357 17.00512817632389 36.94337100361725 33.88935383854951 5 13.272740803354166 38.197585191571335 35.23530972817609 13.294364276898406 6 32.75838015396908 37.763150292479246 17.047643607943936 12.430825945607738 7 29.00945818379799 31.32469439923018 21.116524317928906 18.549323099042926 8 25.527415771754008 37.77978184437594 19.470804485795124 17.22199789807492 9 26.061037533960942 15.531774495141 19.36171021030105 39.04547776059701 10 14.111109083020812 30.19043299883884 34.19748700891159 21.500970909228762 11 35.29650422494479 22.737910228170886 23.218595091791997 18.746990455092323 12 22.746620843792293 25.17239733373765 31.38824778045452 20.69273404201553 13 29.06228635523255 21.658929245766412 25.41149649254077 23.867287906460266 14 20.719015950347973 22.019600075298452 27.30411865687787 29.957265317475702 15 23.096231490164588 30.711445915061127 22.843818262476066 23.348504332298223 16 22.323362641281108 28.81790653371163 26.82287901100768 22.03585181399959 17 21.785019359657227 28.81331899492634 27.31803466487717 22.08362698053926 18 21.697685421115214 28.39574287074045 28.459479075853107 21.44709263229123 19 23.131932384986108 27.48190086856177 27.090086435022116 22.296080311430003 20 23.07337028239641 27.799818062364178 27.692283935019063 21.43452772022035 21 22.775002877847314 27.113392799485638 26.968658137007996 23.142946185659056 22 22.601598396269193 28.226588744965532 26.99908330163026 22.172729557135014 23 22.384988046421718 27.72205368511341 27.1741404022523 22.718817866212575 24 22.58972929947483 27.418763871594482 27.821101529191942 22.170405299738746 25 22.684845277318804 27.440025044255613 27.366745327716156 22.508384350709424 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 84.0 1 84.0 2 96.0 3 108.0 4 108.0 5 108.0 6 181.5 7 255.0 8 255.0 9 255.0 10 513.5 11 772.0 12 772.0 13 772.0 14 1362.5 15 1953.0 16 1953.0 17 1953.0 18 3858.5 19 5764.0 20 5764.0 21 5764.0 22 9683.0 23 13602.0 24 13602.0 25 13602.0 26 20526.5 27 27451.0 28 27451.0 29 27451.0 30 37425.5 31 47400.0 32 47400.0 33 47400.0 34 56433.5 35 65467.0 36 65467.0 37 65467.0 38 73714.0 39 81961.0 40 81961.0 41 81961.0 42 87874.0 43 93787.0 44 93787.0 45 93787.0 46 97078.5 47 100370.0 48 100370.0 49 100370.0 50 96849.5 51 93329.0 52 93329.0 53 93329.0 54 82758.0 55 72187.0 56 72187.0 57 72187.0 58 61068.5 59 49950.0 60 49950.0 61 49950.0 62 40218.5 63 30487.0 64 30487.0 65 30487.0 66 23325.0 67 16163.0 68 16163.0 69 16163.0 70 11614.0 71 7065.0 72 7065.0 73 7065.0 74 4884.5 75 2704.0 76 2704.0 77 2704.0 78 1882.5 79 1061.0 80 1061.0 81 1061.0 82 689.0 83 317.0 84 317.0 85 317.0 86 200.0 87 83.0 88 83.0 89 83.0 90 50.0 91 17.0 92 17.0 93 17.0 94 9.5 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09110830657874973 2 0.029199580536794983 3 0.028638050141856615 4 0.027374606753245295 5 0.02105738981018869 6 0.38127913816314984 7 0.21422384566898625 8 0.21927761922343153 9 0.22405062758040764 10 0.01684591184815095 11 0.0030884171721610072 12 0.004351860560772329 13 0.0 14 0.07089321236096859 15 0.002105738981018869 16 0.002526886777222643 17 0.004632625758241511 18 0.0039307127645685554 19 0.0016845911848150951 20 9.826781911421389E-4 21 1.4038259873459124E-4 22 0.0 23 0.0 24 2.807651974691825E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 712339.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.33239138687236 #Duplication Level Percentage of deduplicated Percentage of total 1 92.60127575811401 80.87090857431309 2 5.858751740328581 10.233176000497906 3 0.8523371243562626 2.233099180135273 4 0.2612294535578439 0.9125517151956969 5 0.1245467528471531 0.5438482882805817 6 0.07341530990863451 0.3846920747237639 7 0.05175742440862613 0.31640697519414185 8 0.03317047859242148 0.23174857751385794 9 0.02127733572658253 0.1672380550209409 >10 0.1054892511540369 1.6622919256248232 >50 0.007891755332317136 0.4732973170418286 >100 0.007247140112414658 1.0800258792236144 >500 0.0016104755610920787 0.8907154372344732 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 745 0.10458503605727049 No Hit CTGTAGGACGTGGAATATGGCAAGA 731 0.10261967967498621 No Hit GTATCAACGCAGAGTACTTTTTTTT 714 0.10023317549649816 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCCGGTT 35 0.0022023008 16.248209 11 CCGGTTT 40 0.00535669 14.217182 12 TAGGACT 115 4.978574E-9 12.44405 4 CTCCTAG 70 1.0496981E-4 12.26281 1 TGGACCG 55 0.0029696051 12.142376 5 CGGTTTC 55 0.0031190275 12.063065 13 AGGACTG 170 0.0 11.785248 5 CTTAGAC 90 7.089373E-6 11.662184 3 AGTACTG 115 6.64495E-8 11.614447 5 TCCTACA 540 0.0 11.486297 2 TGGCGAG 355 0.0 11.4749 18 GGCGAGA 260 0.0 11.294491 19 GTCCTAC 465 0.0 11.281199 1 TAGACTG 110 4.6882997E-7 11.2750635 5 GTAGGAC 600 0.0 11.132086 3 GGATAAT 60 0.0056875814 11.13051 6 TGTAGGA 615 0.0 11.0165 2 GACGTGG 235 0.0 10.915823 7 AGTAGTG 70 0.0014377943 10.903358 5 CCTACAG 500 0.0 10.877639 3 >>END_MODULE