FastQCFastQC Report
Thu 26 May 2016
SRR1926475_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926475_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15477
Sequences flagged as poor quality0
Sequence length25
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC1821.1759384893713252No Hit
GTACATGGGAAGCAGTGGTATCAAC1561.007947276603993No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA970.62673644763197No Hit
GAGTACATGGGAAGCAGTGGTATCA810.5233572397751503No Hit
CATGTACTCTGCGTTGATACCACTG750.4845900368288428No Hit
ACGCAGAGTACATGGGAAGCAGTGG560.3618272274988693No Hit
GTATCAACGCAGAGTACATGGGAAG480.3101376235704594No Hit
ACTCTGCGTTGATACCACTGCTTCC440.28429282160625446No Hit
GCGTTGATACCACTGCTTCCCATGT420.27137042062415195No Hit
GGTATCAACGCAGAGTACATGGGAA420.27137042062415195No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA360.23260321767784456No Hit
CNCATGTACTCTGCGTTGATACCAC360.23260321767784456No Hit
GNACATGGGAAGCAGTGGTATCAAC360.23260321767784456No Hit
GCTTCCCATGTACTCTGCGTTGATA360.23260321767784456No Hit
TATCAACGCAGAGTACATGGGAAGC290.18737481424048588No Hit
CAGTGGTATCAACGCAGAGTACATG270.1744524132583834No Hit
GTACTCTGCGTTGATACCACTGCTT240.1550688117852297No Hit
ANAAAAAAAAAAAAAAAAAAAAAAA230.14860761129417846No Hit
ATACCACTGCTTCCCATGTACTCTG230.14860761129417846No Hit
CNTGTACTCTGCGTTGATACCACTG220.14214641080312723No Hit
GTATCAACGCAGAGTACTTTTTTTT210.13568521031207598No Hit
GTGGTATCAACGCAGAGTACATGGG210.13568521031207598No Hit
CCATGTACTCTGCGTTGATACCACT210.13568521031207598No Hit
CTGCTTCCCATGTACTCTGCGTTGA210.13568521031207598No Hit
CCACTGCTTCCCATGTACTCTGCGT200.12922400982102475No Hit
GGGAAGCAGTGGTATCAACGCAGAG200.12922400982102475No Hit
GCAGAGTACATGGGAAGCAGTGGTA200.12922400982102475No Hit
CATGGGAAGCAGTGGTATCAACGCA190.12276280932997351No Hit
GGTATCAACGCAGAGTACTTTTTTT190.12276280932997351No Hit
GNGTACATGGGAAGCAGTGGTATCA180.11630160883892228No Hit
TACCACTGCTTCCCATGTACTCTGC180.11630160883892228No Hit
GATACCACTGCTTCCCATGTACTCT180.11630160883892228No Hit
TATCAACGCAGAGTACTTTTTTTTT180.11630160883892228No Hit
ACATGGGAAGCAGTGGTATCAACGC170.10984040834787104No Hit
AAGCAGTGGTATCAACGCAGAGTAC160.1033792078568198No Hit
ANGCAGAGTACATGGGAAGCAGTGG160.1033792078568198No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAAGCA800.00327770859.7268918
AGTGGTA905.5881037E-48.71939814
ACATGGG1659.7983604E-57.97931963
GTGGTAT950.00601488867.77715615
TGGTATC1000.0095263467.38829816
Produced by FastQC (version 0.11.5)