FastQCFastQC Report
Thu 26 May 2016
SRR1926475_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926475_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15477
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC4823.1142986366866965No Hit
GTACATGGGAAGCAGTGGTATCAAC4512.9140014214641083No Hit
GTATCAACGCAGAGTACTTTTTTTT2021.3051624991923498No Hit
CATGTACTCTGCGTTGATACCACTG1971.272856496737094No Hit
GAGTACATGGGAAGCAGTGGTATCA1951.2599340957549914No Hit
GGTATCAACGCAGAGTACTTTTTTT1390.898106868256122No Hit
ACGCAGAGTACATGGGAAGCAGTGG1220.7882664599082511No Hit
ACTCTGCGTTGATACCACTGCTTCC1180.7624216579440459No Hit
GCGTTGATACCACTGCTTCCCATGT1110.7171932545066874No Hit
TATCAACGCAGAGTACTTTTTTTTT970.62673644763197No Hit
GTATCAACGCAGAGTACATGGGAAG950.6138140466498675No Hit
ACGCAGAGTACTTTTTTTTTTTTTT870.5621244427214577No Hit
GGTATCAACGCAGAGTACATGGGAA860.5556632422304064No Hit
TATCAACGCAGAGTACATGGGAAGC720.4652064353556891No Hit
CAGTGGTATCAACGCAGAGTACATG660.42643923240938164No Hit
GTGGTATCAACGCAGAGTACATGGG660.42643923240938164No Hit
GCTTCCCATGTACTCTGCGTTGATA610.39413322995412553No Hit
GATACCACTGCTTCCCATGTACTCT540.3489048265167668No Hit
GCAGTGGTATCAACGCAGAGTACAT530.3424436260257156No Hit
CATGGGAAGCAGTGGTATCAACGCA510.3295212250436131No Hit
GCAGAGTACATGGGAAGCAGTGGTA510.3295212250436131No Hit
ACATGGGAAGCAGTGGTATCAACGC500.3230600245525619No Hit
ATACCACTGCTTCCCATGTACTCTG500.3230600245525619No Hit
CTGCTTCCCATGTACTCTGCGTTGA490.31659882406151063No Hit
GTACTCTGCGTTGATACCACTGCTT470.3036764230794082No Hit
CCACTGCTTCCCATGTACTCTGCGT440.28429282160625446No Hit
CTTCCCATGTACTCTGCGTTGATAC420.27137042062415195No Hit
GGGAAGCAGTGGTATCAACGCAGAG420.27137042062415195No Hit
GAGTACTTTTTTTTTTTTTTTTTTT400.2584480196420495No Hit
ATGGGAAGCAGTGGTATCAACGCAG380.24552561865994701No Hit
AAGCAGTGGTATCAACGCAGAGTAC350.22614201718679333No Hit
GTTGATACCACTGCTTCCCATGTAC330.21321961620469082No Hit
CCATGTACTCTGCGTTGATACCACT290.18737481424048588No Hit
TACCACTGCTTCCCATGTACTCTGC290.18737481424048588No Hit
ATCAACGCAGAGTACTTTTTTTTTT280.18091361374943465No Hit
GCAGAGTACTTTTTTTTTTTTTTTT280.18091361374943465No Hit
GTACTTTTTTTTTTTTTTTTTTTTT270.1744524132583834No Hit
GTGGTATCAACGCAGAGTACTTTTT260.16799121276733217No Hit
ATCAACGCAGAGTACATGGGAAGCA240.1550688117852297No Hit
GTACATGGTAAGCAGTGGTATCAAC230.14860761129417846No Hit
GTACATGGAAAGCAGTGGTATCAAC180.11630160883892228No Hit
CTCTGCGTTGATACCACTGCTTCCC170.10984040834787104No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA160.1033792078568198No Hit
GGAAGCAGTGGTATCAACGCAGAGT160.1033792078568198No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACC1201.5877093E-48.65493517
CCATGTA1158.6918834E-48.3452512
CCCATGT1200.00132596047.9975331
GATACCA1200.00156182387.86812318
TTGATAC1200.00156182387.86812316
GAAGCAG1355.282493E-47.74339539
TGCGTTG1408.3718466E-47.41851612
ATGTACT1300.00291826677.38233854
TGTACTC1300.00291826677.38233855
ATACCAC1300.00343205347.262882719
CGTTGAT1300.00343205347.262882714
GTACTCT1350.00422213557.1089186
CATGTAC1350.00422213557.1089183
GTACATG2301.229284E-67.09346341
TGGTATC1604.2980936E-47.081310716
AGTGGTA1604.2980936E-47.081310714
GTGGTAT1604.2980936E-47.081310715
GGAAGCA1350.00465313257.039458
GTTGATA1350.00496164156.99388715
CAGTGGT1656.118172E-46.866725413
Produced by FastQC (version 0.11.5)