FastQCFastQC Report
Thu 26 May 2016
SRR1926474_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926474_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences740642
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG550.004733479614.482731
GTATAGA658.669078E-414.2970541
TTAAGCA450.005274297714.249484
TAGGACC2650.014.1150514
CTGTAGG4600.013.8530451
TGTAGGA4800.013.5964082
GATATAC904.983412E-513.2758371
CATCTAT700.001517082113.2758361
GTGTATA1603.4742698E-1013.2758361
GTGTAAC600.008423421513.2758351
GGTTTCC555.615776E-513.11042914
TCCTAGA1152.089926E-613.005262
GTAGGAC4600.012.78846553
ACTAAAC600.002107103612.7095243
GAAATGC1051.5004223E-512.6436531
GTCCTAG854.579141E-412.4949041
TTAATCC600.002433316312.4682964
TCCAACG554.7519064E-412.44201918
TAGGAGT705.550083E-412.213844
GGACCTT852.1639857E-512.2020546
Produced by FastQC (version 0.11.5)