##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926474_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 740642 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.668362042660288 27.0 10.0 31.0 2.0 31.0 2 16.70216784897427 22.0 2.0 30.0 2.0 31.0 3 20.272987489232314 25.0 15.0 31.0 2.0 31.0 4 22.414854950164855 28.0 7.0 35.0 2.0 37.0 5 22.98827503706244 30.0 7.0 35.0 2.0 37.0 6 23.853508172639412 31.0 8.0 35.0 2.0 37.0 7 23.5937146421618 30.0 10.0 35.0 2.0 37.0 8 23.999927090281133 31.0 12.0 35.0 2.0 37.0 9 26.849211899946262 32.0 17.0 37.0 2.0 39.0 10 27.625954239700153 32.0 25.0 37.0 2.0 39.0 11 24.6859575881465 30.0 10.0 37.0 2.0 39.0 12 25.87217171048901 31.0 17.0 37.0 2.0 39.0 13 22.463179241792933 29.0 2.0 37.0 2.0 39.0 14 25.877156574971444 31.0 17.0 38.0 2.0 40.0 15 27.001559457875736 31.0 18.0 38.0 2.0 40.0 16 27.37393504554157 32.0 22.0 39.0 2.0 40.0 17 26.672153348041295 31.0 17.0 38.0 2.0 40.0 18 26.735618557953774 31.0 17.0 38.0 2.0 40.0 19 27.1498200210088 31.0 21.0 39.0 2.0 40.0 20 27.417456746984374 32.0 24.0 39.0 2.0 40.0 21 25.23123452356199 30.0 10.0 38.0 2.0 40.0 22 26.18032328709417 31.0 16.0 38.0 2.0 40.0 23 26.16751682999344 31.0 10.0 38.0 2.0 40.0 24 25.22269463519487 31.0 2.0 39.0 2.0 40.0 25 25.016875899557412 31.0 2.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.572032801291229 1101 2 10.253692838578582 1101 3 7.010465589092508 1101 4 8.557110706253543 1101 5 8.213445878790637 1101 6 7.6360022719481755 1101 7 7.309948617438103 1101 8 5.970776978509615 1101 9 5.788977889510665 1101 10 5.275515142493518 1101 11 8.053848649832975 1101 12 5.715689842494939 1101 13 10.313857870850416 1101 14 7.64375226564114 1101 15 6.990934160215783 1101 16 6.593124811709508 1101 17 2.832728912272632 1101 18 5.052823776783153 1101 19 5.581817066070226 1101 20 3.613836571160334 1101 21 0.8648539403191684 1101 22 4.42663585671907 1101 23 5.8831408853334395 1101 24 7.3176061207047205 1101 25 7.758208201921267 1102 1 5.364815209363673 1102 2 10.546452673353775 1102 3 6.579390074084198 1102 4 7.751381779306012 1102 5 7.72004812849821 1102 6 6.427632562381493 1102 7 6.600047734734233 1102 8 7.026900058679999 1102 9 5.95493559829168 1102 10 5.378764231499737 1102 11 7.895939933901797 1102 12 5.511331144006764 1102 13 10.035366118898704 1102 14 7.332762587794335 1102 15 5.272877574529598 1102 16 5.763736723960939 1102 17 6.627303698779077 1102 18 6.538388886087748 1102 19 5.205098050852303 1102 20 5.6693762635185365 1102 21 4.616921155976243 1102 22 5.814041715808926 1102 23 6.096346557387406 1102 24 7.486796794708198 1102 25 7.157273022463553 1103 1 4.665816230526225 1103 2 8.988203247794411 1103 3 5.936072346901955 1103 4 7.504895218545077 1103 5 6.550677250363009 1103 6 6.304170545341762 1103 7 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2205 20 5.790048443039737 2205 21 -0.5099128399853505 2205 22 4.0935425295262995 2205 23 5.548224484976238 2205 24 7.517418534002033 2205 25 7.840479011970071 2206 1 4.3596179886333815 2206 2 8.739067214898041 2206 3 5.993222607073548 2206 4 1.8976976799399665 2206 5 5.82988696582872 2206 6 6.278817976098207 2206 7 6.229392330794248 2206 8 6.321986260780008 2206 9 4.971450996349734 2206 10 4.135059204718779 2206 11 3.620806880736172 2206 12 4.801775956608179 2206 13 8.133640373636329 2206 14 0.32122752841294044 2206 15 2.7070090186802 2206 16 3.3614443601395934 2206 17 4.689003236513717 2206 18 -1.3205378543339563 2206 19 0.8463808116813603 2206 20 2.6033839372825085 2206 21 3.320281010994563 2206 22 -1.881252057178017 2206 23 -2.522140309893267 2206 24 1.607823225931039 2206 25 3.2809031738381407 2207 1 1.5717046592004955 2207 2 -13.952390811611531 2207 3 -6.225713378562057 2207 4 -3.9183683406875573 2207 5 1.1521006698387808 2207 6 1.5817043050401765 2207 7 2.8305920537281892 2207 8 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2208 21 6.789349459548514 2208 22 5.78290002617965 2208 23 1.920551825047955 2208 24 3.2168410041005124 2208 25 4.5344531269584785 2209 1 2.8212519439743495 2209 2 4.591155067082093 2209 3 4.907949460441756 2209 4 0.6553964272973687 2209 5 4.97268341197929 2209 6 5.476532344268396 2209 7 5.98696166819235 2209 8 4.590612705762453 2209 9 4.029092253619023 2209 10 3.2614537974438775 2209 11 -0.8841771205281503 2209 12 3.031157972517587 2209 13 1.862418858142636 2209 14 3.8316192626667274 2209 15 4.209559425577176 2209 16 3.598966606055164 2209 17 1.0606251212266216 2209 18 3.3304961655974665 2209 19 3.968177342578276 2209 20 4.033700682473157 2209 21 6.146498323527631 2209 22 4.69627023454639 2209 23 0.30517755990427986 2209 24 -3.4181045747638805 2209 25 -0.008379620134331134 2210 1 -1.4268226211772692 2210 2 -13.952390811611531 2210 3 -7.399517670487814 2210 4 -1.8511349237182948 2210 5 -0.01768675983690926 2210 6 -0.5992337160610113 2210 7 -0.5254242786545014 2210 8 -21.239869409323084 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-3.7842046858490264 2211 22 -5.437418155337873 2211 23 -7.934243056290917 2211 24 -22.540188556101363 2211 25 -22.38551803382018 2212 1 -0.583385532389574 2212 2 -13.952390811611531 2212 3 -7.5555058127216 2212 4 -19.66342161313809 2212 5 -20.17894955847172 2212 6 -21.01646921094156 2212 7 -9.58837262073561 2212 8 -9.339086119505852 2212 9 -4.899397189496703 2212 10 -5.05057830295582 2212 11 -5.02138886865723 2212 12 -2.301660341331633 2212 13 -6.064143830083111 2212 14 -3.3805538759202136 2212 15 -3.4021138531004773 2212 16 -3.6638577851593226 2212 17 -3.688132673434229 2212 18 -2.676644400206591 2212 19 -3.3728467679133693 2212 20 -3.64891331549925 2212 21 -1.4406496054984892 2212 22 -2.9177575155967475 2212 23 -3.0936695002887085 2212 24 -1.920519278468639 2212 25 -2.649225605621748 2213 1 -18.996788491484438 2213 2 -13.952390811611531 2213 3 -8.778148690586411 2213 4 -6.08194013165661 2213 5 -20.17894955847172 2213 6 -10.94332106279341 2213 7 -20.815526667733 2213 8 -11.029684224137899 2213 9 -8.676604422839706 2213 10 -7.671562733861922 2213 11 -6.923506652237155 2213 12 -5.449871346070051 2213 13 -19.563708669073538 2213 14 -12.878269280619952 2213 15 -7.54872926747046 2213 16 -6.81152242107461 2213 17 -5.454132093219549 2213 18 -5.510765858479488 2213 19 -6.424410929986667 2213 20 -6.282551261539286 2213 21 -4.45354487674885 2213 22 -5.289960397329654 2213 23 -5.058215965814284 2213 24 -4.219818185730993 2213 25 -5.1521847004868455 2214 1 -18.996788491484438 2214 2 -13.952390811611531 2214 3 -12.228999945066697 2214 4 -19.66342161313809 2214 5 -20.17894955847172 2214 6 -21.01646921094156 2214 7 -16.321575054829772 2214 8 -15.043296828677923 2214 9 -13.953471208980687 2214 10 -13.96422253075559 2214 11 -14.74705055904719 2214 12 -12.396048168053326 2214 13 -19.563708669073538 2214 14 -17.00864562470597 2214 15 -24.324655193396385 2214 16 -18.53004840673769 2214 17 -24.03190987099733 2214 18 -18.077425058001587 2214 19 -15.162149878612714 2214 20 -13.79626093895864 2214 21 -11.298213335530212 2214 22 -12.397606753363108 2214 23 -12.276107244189435 2214 24 -11.038172427069105 2214 25 -10.847211582207276 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 121936.0 3 7.0 4 508.0 5 2111.0 6 2759.0 7 2709.0 8 2376.0 9 2364.0 10 2294.0 11 2116.0 12 2059.0 13 2142.0 14 3416.0 15 7550.0 16 11086.0 17 11720.0 18 11970.0 19 13697.0 20 16373.0 21 19222.0 22 20490.0 23 22471.0 24 23481.0 25 23993.0 26 20021.0 27 15846.0 28 16901.0 29 19924.0 30 19181.0 31 22040.0 32 29046.0 33 36760.0 34 44763.0 35 50779.0 36 50321.0 37 51641.0 38 34569.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.704526236561136 18.320835307928427 11.760223756469703 26.214414699040734 2 14.084028680447819 20.616168392804727 40.76753742295274 24.532265503794708 3 19.55196445149229 23.864046352242248 28.673533559884717 27.91045563638075 4 11.368534482758621 17.205822070952998 38.25167072443605 33.17397272185233 5 12.475714294581728 37.51559561274464 36.23597946655245 13.772710626121176 6 31.703809417530206 38.09480399661134 17.81075979309662 12.39062679276184 7 27.919054547614095 30.786441785194647 21.981916441488256 19.312587225703002 8 24.568523774744556 37.60880752348172 20.860138871273648 16.962529830500074 9 26.207152897923873 15.364943771626297 19.87862240484429 38.54928092560554 10 13.548589075985241 29.886762428038843 35.442006586727295 21.122641909248625 11 33.70849345395516 22.190079225829276 24.685821229783432 19.41560609043213 12 23.23577777967778 24.691131080122094 32.200172710094996 19.872918430105134 13 28.194079372603092 21.405259268834914 25.226105150265965 25.17455620829603 14 20.058480973149674 22.196075532447907 27.962721117490357 29.782722376912062 15 22.254376558101853 31.714595278491846 23.375863068772205 22.655165094634093 16 21.609845907766815 28.369743605408367 28.170359530031185 21.850050956793638 17 21.232374299235833 28.58313562412556 28.82357590438963 21.360914172248975 18 21.022631676139277 27.71255240713763 30.622046220872573 20.642769695850518 19 22.735165198749833 27.151767379160045 27.196884972013418 22.9161824500767 20 22.855134947759538 27.8748503739973 28.43246112432093 20.837553553922234 21 21.873376810757776 26.465533138046222 26.88937468240037 24.771715368795636 22 22.175251649255763 28.508612818129425 27.97129562575892 21.344839906855896 23 21.79953151842906 27.97062055560887 28.267172363642263 21.96267556231981 24 22.22187711225786 27.47543679828668 28.61903261732876 21.6836534721267 25 22.250337587133977 27.57792889786418 28.34150436126081 21.830229153741033 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 551.0 1 551.0 2 1260.5 3 1970.0 4 1970.0 5 1970.0 6 2847.5 7 3725.0 8 3725.0 9 3725.0 10 4399.5 11 5074.0 12 5074.0 13 5074.0 14 6354.0 15 7634.0 16 7634.0 17 7634.0 18 11149.0 19 14664.0 20 14664.0 21 14664.0 22 20816.5 23 26969.0 24 26969.0 25 26969.0 26 36193.5 27 45418.0 28 45418.0 29 45418.0 30 55798.5 31 66179.0 32 66179.0 33 66179.0 34 74087.5 35 81996.0 36 81996.0 37 81996.0 38 87123.0 39 92250.0 40 92250.0 41 92250.0 42 93318.5 43 94387.0 44 94387.0 45 94387.0 46 93005.5 47 91624.0 48 91624.0 49 91624.0 50 84603.5 51 77583.0 52 77583.0 53 77583.0 54 66403.0 55 55223.0 56 55223.0 57 55223.0 58 45919.5 59 36616.0 60 36616.0 61 36616.0 62 28926.0 63 21236.0 64 21236.0 65 21236.0 66 15925.0 67 10614.0 68 10614.0 69 10614.0 70 7545.0 71 4476.0 72 4476.0 73 4476.0 74 3078.0 75 1680.0 76 1680.0 77 1680.0 78 1118.0 79 556.0 80 556.0 81 556.0 82 360.0 83 164.0 84 164.0 85 164.0 86 102.5 87 41.0 88 41.0 89 41.0 90 25.0 91 9.0 92 9.0 93 9.0 94 5.5 95 2.0 96 2.0 97 2.0 98 1.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 12.818608720542448 2 35.59911536207777 3 5.320114171219024 4 13.04408877703398 5 12.992781937832312 6 14.09628403466182 7 9.059977695026747 8 7.383189179117576 9 0.1082844343150942 10 1.2382500587328291 11 5.347792860788343 12 5.250579902300976 13 15.399207714388327 14 1.3701626426802693 15 2.6751115923752637 16 2.757607589091626 17 2.62812532910637 18 1.294417545858863 19 1.244865940629886 20 0.288128407516722 21 4.082269166479892 22 1.3719178766529578 23 0.10868948830879156 24 8.71257638643231 25 0.11246999224996691 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 740642.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 94.01214594593476 #Duplication Level Percentage of deduplicated Percentage of total 1 97.22849698985155 91.40659649113802 2 2.106951063154591 3.961579817004636 3 0.31229599884745146 0.8807885106593423 4 0.12283050224886184 0.46190236416129865 5 0.06232112656461537 0.2929471423053846 6 0.035920016998846435 0.20261507282856045 7 0.02121173950163946 0.1395912804856695 8 0.0189183614527106 0.142284460636014 9 0.01413246640729925 0.11957611450131338 >10 0.06621367565802355 1.0955983766736581 >50 0.006747750112847039 0.45618674454100383 >100 0.0036669529708448848 0.6643792938421 >500 2.9335623076716524E-4 0.17595433122298448 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 1.3501799789911994E-4 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATTAG 55 0.0047334796 14.48273 1 GTATAGA 65 8.669078E-4 14.297054 1 TTAAGCA 45 0.0052742977 14.24948 4 TAGGACC 265 0.0 14.115051 4 CTGTAGG 460 0.0 13.853045 1 TGTAGGA 480 0.0 13.596408 2 GATATAC 90 4.983412E-5 13.275837 1 CATCTAT 70 0.0015170821 13.275836 1 GTGTATA 160 3.4742698E-10 13.275836 1 GTGTAAC 60 0.0084234215 13.275835 1 GGTTTCC 55 5.615776E-5 13.110429 14 TCCTAGA 115 2.089926E-6 13.00526 2 GTAGGAC 460 0.0 12.7884655 3 ACTAAAC 60 0.0021071036 12.709524 3 GAAATGC 105 1.5004223E-5 12.643653 1 GTCCTAG 85 4.579141E-4 12.494904 1 TTAATCC 60 0.0024333163 12.468296 4 TCCAACG 55 4.7519064E-4 12.442019 18 TAGGAGT 70 5.550083E-4 12.21384 4 GGACCTT 85 2.1639857E-5 12.202054 6 >>END_MODULE