FastQCFastQC Report
Thu 26 May 2016
SRR1926474_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926474_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences740642
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA13950.18835010706927233No Hit
GTCCTACAGTGGACATTTCTAAATT13720.18524469311759256No Hit
GTCCTACAGTGTGCATTTCTCATTT10260.13852846584449707No Hit
GTCCTAAAGTGTGTATTTCTCATTT10200.13771835785710235No Hit
CTTTAGGACGTGAAATATGGCGAGG9670.13056240396844898No Hit
CTGTAGGACCTGGAATATGGCGAGA9120.1231364140839974No Hit
GTATCAACGCAGAGTACTTTTTTTT7850.10598912835080916No Hit
CCCATGTACTCTGCGTTGATACCAC7770.1049089843676162No Hit
GTACATGGGAAGCAGTGGTATCAAC7620.10288371439912941No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT350.002116133516.3456926
GACCGTG350.002167563716.2870487
CCATAAT551.9000225E-413.8671871
TAGGACC2450.012.453864
GTATTAA853.782634E-612.3377181
TACACCT701.0526448E-412.2592695
GTAGGAC7200.012.053623
CCTACAC1209.416908E-911.9211643
TGTAGGA8400.011.3550312
AGGACGT6600.011.26862055
GGACGTG6550.011.2090686
GTCCTAC7000.011.1680381
GTCCTAA3100.011.0713831
GGCGAGG2950.010.92121819
GCTCTAG1053.313935E-610.8956461
CTAAATT4100.010.8624619
TAGGACG6850.010.8573574
TCCTACA8000.010.7305052
GCTGGAC803.632965E-410.7290463
TTAGACA803.6398176E-410.726864
Produced by FastQC (version 0.11.5)