FastQCFastQC Report
Thu 26 May 2016
SRR1926473_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926473_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences678183
Sequences flagged as poor quality0
Sequence length25
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTAA500.002499939215.9399481
GTCCTAC3750.014.8772851
TCCTACA4350.014.7010932
GTCTAGT550.004714843414.4908621
TGTAGGA3200.014.4567262
TAGGACC1700.014.4525494
GTATAAT751.5797121E-414.1688431
GACGTGC450.007299144813.5673287
CTCTTAG700.001510246113.2832911
GTAGGAC3700.013.2575163
AGGACGT3150.013.1924965
TTTAGAG500.00929054413.08074953
GCTGTAC500.00929054413.08074953
GCTAAAC500.00929054413.08074953
ACTGCGT450.0095866113.0191288
TGTAGAA1152.0714597E-613.01475052
GGACGTG3050.012.9385626
CTGTAGG3350.012.6885171
ACGTGAA1551.8189894E-1212.5991568
TTAGACA600.002440968512.4627054
Produced by FastQC (version 0.11.5)