FastQCFastQC Report
Thu 26 May 2016
SRR1926473_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926473_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences678183
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT11610.17119273116548187No Hit
CTGTAGGACGTGGAATATGGCAAGA11530.17001310855624513No Hit
GTCCTACAGTGTGCATTTCTCATTT8450.12459763810063065No Hit
CTTTAGGACGTGAAATATGGCGAGG7650.11280141200826325No Hit
GTCCTAAAGTGTGTATTTCTCATTT7420.10940999700670762No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTAG456.5862376E-414.8272615
TAGGACC3200.013.9998464
TCCTATA551.9004372E-413.8665092
GTACTAT551.9100268E-413.8582871
GTCCTAC4900.013.2219371
GTAGGAC6450.012.8568613
GTGTAAG853.8085527E-612.3297991
GTCCTAT701.0600647E-412.2497361
CTAGCAG550.002989551312.1313953
CGGTTTC550.003104341412.07053313
TGTAGGA6650.011.8986112
TCTAAAT3200.011.84712118
TCCTACA5400.011.8282592
CCCAGTA657.781786E-411.73322
CTAAATT2750.011.71787919
CTGTAGG7000.011.7053021
TCTTACA907.1592094E-611.6517192
GACGTGG2450.011.6345097
CTACACT1156.726805E-811.6039434
CTATACT752.0071949E-411.4381734
Produced by FastQC (version 0.11.5)