##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926473_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 678183 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.699530952560004 31.0 28.0 31.0 23.0 34.0 2 29.121309440077383 31.0 28.0 33.0 25.0 34.0 3 29.50431078337263 31.0 30.0 33.0 25.0 34.0 4 33.3442359952992 35.0 33.0 37.0 28.0 37.0 5 33.07639678375896 35.0 33.0 37.0 27.0 37.0 6 33.20120675392925 35.0 33.0 37.0 28.0 37.0 7 33.03403801038952 35.0 33.0 37.0 26.0 37.0 8 33.00085670091996 35.0 33.0 37.0 26.0 37.0 9 34.288037299666904 38.0 34.0 39.0 26.0 39.0 10 34.194295639967386 37.0 34.0 39.0 26.0 39.0 11 34.46488042313063 38.0 35.0 39.0 27.0 39.0 12 34.26073051079133 38.0 34.0 39.0 27.0 39.0 13 34.408414837882994 38.0 35.0 39.0 27.0 39.0 14 35.09933012181078 39.0 34.0 40.0 26.0 41.0 15 35.23324088041134 39.0 35.0 40.0 27.0 41.0 16 35.21463675733541 39.0 35.0 40.0 27.0 41.0 17 35.11581239871834 39.0 34.0 40.0 26.0 41.0 18 35.08858081078411 39.0 34.0 40.0 26.0 41.0 19 35.12735795500625 39.0 34.0 40.0 26.0 41.0 20 35.1092301045588 39.0 34.0 41.0 26.0 41.0 21 35.02682314360578 39.0 34.0 41.0 25.0 41.0 22 34.96884616689006 39.0 34.0 40.0 25.0 41.0 23 34.62933603466911 39.0 34.0 40.0 23.0 41.0 24 34.71708963509849 39.0 34.0 41.0 24.0 41.0 25 34.51071318508426 39.0 34.0 40.0 23.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7634814289430913 1101 2 0.8458716174756731 1101 3 0.9553910156450058 1101 4 0.8021494560211622 1101 5 0.8702291106974442 1101 6 0.23474978886883235 1101 7 0.7250645133804952 1101 8 0.75179082015012 1101 9 0.9484348894896684 1101 10 1.0336260881623858 1101 11 0.5752794714267395 1101 12 0.7323946447116185 1101 13 0.7755023201322544 1101 14 1.0149157493999397 1101 15 0.865888024805578 1101 16 1.0094453475625542 1101 17 0.8176979494721266 1101 18 1.0315962032084514 1101 19 0.7813358395575705 1101 20 0.8342790663839494 1101 21 0.754059417608687 1101 22 0.8386225999120072 1101 23 0.9994731910281658 1101 24 0.9189087325194194 1101 25 0.8988556608206864 1102 1 0.5126724073372841 1102 2 0.4112486824380781 1102 3 0.30698194648380905 1102 4 0.21464970974658115 1102 5 0.15472634291564447 1102 6 -0.16708674817073188 1102 7 0.12657700681987905 1102 8 0.30082724352070045 1102 9 0.7443200432182664 1102 10 0.741681652578194 1102 11 0.35938741636687865 1102 12 0.33292137867109517 1102 13 0.26770207597591167 1102 14 0.48193289543849005 1102 15 0.5076893592562755 1102 16 0.4043392323451229 1102 17 0.4520245332261581 1102 18 0.4003817428896568 1102 19 0.5118758252329627 1102 20 0.5324502135997804 1102 21 0.6579540302207292 1102 22 0.6154710982925167 1102 23 0.7686294597857213 1102 24 0.8052890422674039 1102 25 0.7696507057808333 1103 1 0.4611541227304521 1103 2 0.49538560238816487 1103 3 0.219227043105942 1103 4 0.3438963786354634 1103 5 0.3764720014830232 1103 6 -0.0321557302681299 1103 7 0.4694816000216733 1103 8 0.469926302225538 1103 9 0.7782616835084184 1103 10 1.052091503876568 1103 11 0.7108871169577284 1103 12 0.59866027029679 1103 13 0.40422527207267933 1103 14 0.7905219556477547 1103 15 0.844941314265256 1103 16 0.8100179766556082 1103 17 0.7872079102663463 1103 18 0.7574964711647922 1103 19 0.9721495412397019 1103 20 0.7738005341003102 1103 21 0.7665554797739702 1103 22 0.6310735596421893 1103 23 1.0226405099006186 1103 24 0.8258671526564569 1103 25 0.8619387816272166 1104 1 0.5392596659651439 1104 2 0.7420218139940822 1104 3 0.6209409338746248 1104 4 0.2771984969744352 1104 5 0.5241446567150945 1104 6 0.05394859514890982 1104 7 0.3561120111217022 1104 8 0.5643309784144606 1104 9 0.8572286451543718 1104 10 0.8411947774108199 1104 11 0.4551684963238003 1104 12 0.5143372196347045 1104 13 0.31756903284942695 1104 14 0.9051952154599121 1104 15 0.6014448789791302 1104 16 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0.3277033943845922 2202 21 0.612881753901263 2202 22 0.39825611541434114 2202 23 0.8481419578627225 2202 24 0.6410135432844584 2202 25 0.6905071388575763 2203 1 -0.09123248301482079 2203 2 0.11475765068480115 2203 3 -0.10376993742223206 2203 4 -0.41905755819948354 2203 5 0.004785385222916716 2203 6 -0.18253124398476217 2203 7 0.032969183198837015 2203 8 -0.12353749953484083 2203 9 0.30977970451272796 2203 10 0.2942197356025744 2203 11 0.012646375043154023 2203 12 0.14166332108938917 2203 13 0.018892492570188324 2203 14 0.5336722140814345 2203 15 0.19866472050390627 2203 16 0.09653445772183034 2203 17 0.2887512041529021 2203 18 0.3031923628486055 2203 19 0.4311152248566188 2203 20 0.4419785524348967 2203 21 0.4323270185860082 2203 22 0.5732897132571608 2203 23 0.8017373231609426 2203 24 0.5058076133552234 2203 25 0.4956274068913018 2204 1 0.276837803318962 2204 2 0.2559385542443202 2204 3 0.0806159720424553 2204 4 -0.21079350422342458 2204 5 -0.07711239786176094 2204 6 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18 0.8235306025962785 2205 19 0.7068360449287354 2205 20 0.7892535497207476 2205 21 0.6962284465125705 2205 22 0.6660299437595967 2205 23 1.1027868406143284 2205 24 1.0061654520949475 2205 25 0.981848115540572 2206 1 0.58565512374674 2206 2 0.70045225750237 2206 3 0.5950651589937301 2206 4 0.523922357475989 2206 5 0.8862964490065437 2206 6 0.5614707184357357 2206 7 0.44521148257714316 2206 8 0.48411168660656045 2206 9 0.793776548191893 2206 10 0.8744404086532498 2206 11 0.7019876951254176 2206 12 0.5511796425718671 2206 13 0.5381441354238348 2206 14 0.7562857072820819 2206 15 0.5901860430392034 2206 16 0.8348150569123263 2206 17 0.8156880486686546 2206 18 0.6242558416217818 2206 19 0.6173728932203204 2206 20 0.6140221425584187 2206 21 0.7442682197036206 2206 22 0.7648908654702353 2206 23 0.9850038599921263 2206 24 0.8221940372054419 2206 25 0.8893451090160056 2207 1 -0.9472194771256888 2207 2 0.08004463726777189 2207 3 0.44432246461377645 2207 4 0.3136000498982128 2207 5 0.2483396237182589 2207 6 0.43932803067343684 2207 7 0.47278437333613255 2207 8 0.5022840545704739 2207 9 0.7501696206056323 2207 10 0.6321803125868968 2207 11 0.4771892579959385 2207 12 0.4417469965559704 2207 13 0.08542317453972004 2207 14 -5.021185161230541 2207 15 -1.7352642335238073 2207 16 -0.4607282253363536 2207 17 -0.555030463681554 2207 18 -0.09227121081764977 2207 19 0.4217900428841972 2207 20 0.4796064542942844 2207 21 0.5945850401389521 2207 22 0.594999009051385 2207 23 1.0386062103640938 2207 24 0.7758607718869399 2207 25 0.5257722899483213 2208 1 0.3027620414164929 2208 2 0.7515426650322006 2208 3 0.5747348915460115 2208 4 0.4729082361279424 2208 5 0.5458402705692862 2208 6 0.5262568876216775 2208 7 0.42202306928447086 2208 8 0.5777161246521629 2208 9 0.7677778295411528 2208 10 0.7654804673030995 2208 11 0.6504007809208687 2208 12 0.7122798961910988 2208 13 0.38279379113395606 2208 14 0.8485357423543363 2208 15 0.6632177787373195 2208 16 0.7770819331289474 2208 17 0.35483546654302245 2208 18 0.6530965437908307 2208 19 0.4164059981907542 2208 20 0.47942163971611507 2208 21 0.633483228428048 2208 22 0.5993078289310958 2208 23 0.9427607100710276 2208 24 0.7817339151604799 2208 25 0.885834651667416 2209 1 -0.22183981494513105 2209 2 -0.007083838283520549 2209 3 0.4957396136260037 2209 4 0.6256702296977039 2209 5 0.590779818787091 2209 6 0.6568037358448962 2209 7 0.8385783792308104 2209 8 0.9456602367609719 2209 9 0.7876638502139528 2209 10 0.5029679266631035 2209 11 0.5944865249017113 2209 12 0.5784520141334752 2209 13 0.8137560935885162 2209 14 1.0510349895690254 2209 15 0.848819250727459 2209 16 0.8577825993265975 2209 17 0.9015643542108052 2209 18 1.065867112868773 2209 19 0.9383540261707779 2209 20 0.8956605716906836 2209 21 0.877458517643305 2209 22 0.9636767311966707 2209 23 1.0683266866729397 2209 24 1.0797591893200291 2209 25 1.214126751792712 2210 1 -0.41857340437278623 2210 2 -0.6556813594837827 2210 3 0.06850422429015879 2210 4 0.22076578187088103 2210 5 0.48088153171985226 2210 6 0.2690696873802523 2210 7 0.5383899324670196 2210 8 0.5982924514936414 2210 9 0.8276580518519907 2210 10 0.9302419717453674 2210 11 0.3730712413859649 2210 12 0.13970446031742512 2210 13 0.3098156234380056 2210 14 0.7954117864572368 2210 15 0.36829449896983135 2210 16 0.463670352702934 2210 17 0.4612270828233065 2210 18 0.5362109074134764 2210 19 0.4961878547808567 2210 20 0.47519960191464605 2210 21 0.6263677007990651 2210 22 0.6777546708453812 2210 23 0.8233990453982614 2210 24 0.6655193787331868 2210 25 0.5918030582361453 2211 1 -0.9158683531334759 2211 2 -0.15628002178894462 2211 3 0.665075180186335 2211 4 0.7294733313660089 2211 5 1.0435472291635293 2211 6 0.9405131194322891 2211 7 1.0983677071811613 2211 8 1.2168480585739871 2211 9 1.3730097502899525 2211 10 1.385987333470247 2211 11 1.1549425937444795 2211 12 1.310556749897863 2211 13 1.1903388367464416 2211 14 1.3404242568478963 2211 15 1.1817265927839316 2211 16 1.451076302562356 2211 17 1.4347489133179039 2211 18 1.4008915024829705 2211 19 1.2812810380517377 2211 20 1.3040966512155094 2211 21 1.261400098748652 2211 22 1.475618202576996 2211 23 1.5357762736711678 2211 24 1.3129377780948417 2211 25 1.37040746068093 2212 1 1.3255298864475584 2212 2 0.6643447331450503 2212 3 0.7079940684091923 2212 4 0.6490357955920061 2212 5 0.7308759083084695 2212 6 0.7937069773458347 2212 7 0.8331961497106519 2212 8 0.9601162362744446 2212 9 1.164383426933533 2212 10 0.8240224787521342 2212 11 0.7163377685920338 2212 12 1.043805051900307 2212 13 0.6610224075215001 2212 14 0.7658011514616234 2212 15 0.7084434069033421 2212 16 0.1005059466476439 2212 17 0.7315467175264985 2212 18 0.7931918103084072 2212 19 0.5053247736012949 2212 20 0.7553759520957186 2212 21 0.7964331573075967 2212 22 0.9532163529202009 2212 23 1.3023472425325764 2212 24 1.1070291522221822 2212 25 0.4741522945768821 2213 1 0.09633112465935056 2213 2 -0.047197169628585556 2213 3 -0.5452058452039381 2213 4 -1.4382231696167125 2213 5 -2.322811474066551 2213 6 -1.0275843006656231 2213 7 -0.5520944289785348 2213 8 -0.5519501582050239 2213 9 -1.169466856394834 2213 10 -0.9519259770825741 2213 11 -0.7087668726453558 2213 12 -0.9147878705469097 2213 13 -0.9785839151745961 2213 14 -8.223005578486852 2213 15 -3.781948110728834 2213 16 -2.44178145423885 2213 17 -1.7907914870664001 2213 18 -5.9449284525544925 2213 19 -3.1532702056761437 2213 20 -5.458582726185181 2213 21 -2.707981828757376 2213 22 -2.2210537294558534 2213 23 -4.876977500106545 2213 24 -2.6006191709469846 2213 25 -1.9666511033066456 2214 1 0.3819930986917015 2214 2 0.019883312213039517 2214 3 -1.7259723708310801 2214 4 -1.9167870225634722 2214 5 -0.4929274688417635 2214 6 -0.6881349130153254 2214 7 -0.6589826653890611 2214 8 -0.5990313098913589 2214 9 -5.361055839682205 2214 10 -1.5645447349672068 2214 11 -0.9319222877816173 2214 12 -1.1914996028769664 2214 13 -1.600398524487666 2214 14 -2.7383952606455395 2214 15 -2.191596107334952 2214 16 -1.661702635844911 2214 17 -1.9443891113505032 2214 18 -1.3486326937878985 2214 19 -4.672210064785471 2214 20 -2.4106207287082597 2214 21 -4.041832431059408 2214 22 -6.406537078232827 2214 23 -6.510007542641684 2214 24 -6.240079722675741 2214 25 -4.085021594414453 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 17488.0 3 6.0 4 312.0 5 2071.0 6 2682.0 7 2122.0 8 1633.0 9 1360.0 10 1246.0 11 1121.0 12 1075.0 13 1068.0 14 1105.0 15 1112.0 16 1186.0 17 1240.0 18 1417.0 19 1695.0 20 2132.0 21 2814.0 22 3652.0 23 4524.0 24 5612.0 25 6667.0 26 7668.0 27 8598.0 28 10071.0 29 12223.0 30 14983.0 31 19190.0 32 25160.0 33 32340.0 34 43305.0 35 57254.0 36 78165.0 37 116054.0 38 187832.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.757421738950775 18.022178145347432 8.739027514595547 30.481372601106248 2 14.36770250098824 20.2893334827987 38.37459364103532 26.968370375177734 3 19.085926345475357 23.004059140736548 28.341268717476726 29.568745796311376 4 12.611871176321362 16.15871105526156 35.80157873311977 35.42783903529731 5 13.81100497169042 37.795761010797314 34.53577154116504 13.857462476347232 6 34.18905999804653 36.52078813476548 16.447592353068273 12.842559514119712 7 30.164631614412396 30.58193677472171 20.237822565510555 19.015609045355337 8 26.514443964982178 37.336482021150196 18.669423246301083 17.479650767566547 9 26.470644741295096 15.290567331551047 18.635531338532406 39.60325658862145 10 14.916014098423513 29.307761506584672 33.22730021088646 22.548924184105353 11 36.30269696388811 21.97620065765221 22.77968650928233 18.941415869177344 12 23.819052351720753 24.06825497704829 30.61668065052207 21.496012020708886 13 29.861261635871145 21.103153573592966 24.952409600358607 24.083175190177283 14 21.230933138157923 21.236245372085097 26.35753400198618 31.1752874877708 15 23.560429449209714 30.567806644636825 22.158423170557278 23.713340735596184 16 23.219423001776853 27.690606268386013 26.460079774096272 22.62989095574086 17 22.49820099328764 28.38286991706874 26.75992992721396 22.35899916242966 18 22.532588179780582 27.392355786245133 28.22416538869883 21.85089064527545 19 24.20661486058068 26.629901057257033 26.108645324918534 23.054838757243758 20 24.132231161232482 27.040768178172037 26.801007408106447 22.02599325248903 21 23.459744641195666 26.31649569511474 26.215490509198847 24.00826915449075 22 23.711741521093867 27.483584814128342 25.907903913840364 22.89676975093743 23 23.068994651885998 27.262405574896455 26.46394852126933 23.20465125194822 24 23.752578596506233 26.39760299272021 27.139737443949162 22.710080966824393 25 23.3164204941734 26.85661539731901 26.81061011555878 23.016353992948808 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 65.0 1 65.0 2 67.5 3 70.0 4 70.0 5 70.0 6 121.0 7 172.0 8 172.0 9 172.0 10 350.0 11 528.0 12 528.0 13 528.0 14 938.0 15 1348.0 16 1348.0 17 1348.0 18 2749.0 19 4150.0 20 4150.0 21 4150.0 22 7160.5 23 10171.0 24 10171.0 25 10171.0 26 15954.0 27 21737.0 28 21737.0 29 21737.0 30 30627.5 31 39518.0 32 39518.0 33 39518.0 34 47741.0 35 55964.0 36 55964.0 37 55964.0 38 63717.5 39 71471.0 40 71471.0 41 71471.0 42 78083.5 43 84696.0 44 84696.0 45 84696.0 46 89728.5 47 94761.0 48 94761.0 49 94761.0 50 92873.5 51 90986.0 52 90986.0 53 90986.0 54 82628.0 55 74270.0 56 74270.0 57 74270.0 58 65179.5 59 56089.0 60 56089.0 61 56089.0 62 46217.0 63 36345.0 64 36345.0 65 36345.0 66 28406.0 67 20467.0 68 20467.0 69 20467.0 70 14976.5 71 9486.0 72 9486.0 73 9486.0 74 6662.5 75 3839.0 76 3839.0 77 3839.0 78 2630.0 79 1421.0 80 1421.0 81 1421.0 82 948.5 83 476.0 84 476.0 85 476.0 86 303.0 87 130.0 88 130.0 89 130.0 90 76.5 91 23.0 92 23.0 93 23.0 94 11.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08537518634350905 2 0.031112546318619018 3 0.030522735014000643 4 0.025214433272435315 5 0.02123320696626132 6 0.3628814051664521 7 0.21528112618570505 8 0.22103178640573415 9 0.22471810705959896 10 0.0137131128323771 11 0.0019168867400097024 12 0.0029490565230918496 13 0.0 14 0.07402131872960543 15 0.0017694339138551098 16 0.002654150870782665 17 0.004571037610792367 18 0.003391415001555627 19 0.0019168867400097024 20 0.0010321697830821476 21 0.0 22 0.0 23 0.0 24 5.8981130461837E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 678183.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.1640223817667 #Duplication Level Percentage of deduplicated Percentage of total 1 91.40948180358326 76.93389672422477 2 6.315627844766699 10.63097286563707 3 1.209215945462131 3.053174336947919 4 0.41877152787913674 1.4098198498106522 5 0.20339716410559075 0.8559361736085406 6 0.10871327165068118 0.5489847737041809 7 0.07726493549502855 0.4552049432230541 8 0.0496709067945959 0.3344402648946414 9 0.031374340485326606 0.23765316253381777 >10 0.15379722749077818 2.27156180338976 >50 0.011254784772073322 0.6377604269555366 >100 0.00984759786223658 1.5623133456583211 >500 0.001230949729713256 0.7258037738203553 >1k 3.5169992277521596E-4 0.34247755559141563 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1161 0.17119273116548187 No Hit CTGTAGGACGTGGAATATGGCAAGA 1153 0.17001310855624513 No Hit GTCCTACAGTGTGCATTTCTCATTT 845 0.12459763810063065 No Hit CTTTAGGACGTGAAATATGGCGAGG 765 0.11280141200826325 No Hit GTCCTAAAGTGTGTATTTCTCATTT 742 0.10940999700670762 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 1.474528261545925E-4 0.0 0.0 1.474528261545925E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAAGTAG 45 6.5862376E-4 14.827261 5 TAGGACC 320 0.0 13.999846 4 TCCTATA 55 1.9004372E-4 13.866509 2 GTACTAT 55 1.9100268E-4 13.858287 1 GTCCTAC 490 0.0 13.221937 1 GTAGGAC 645 0.0 12.856861 3 GTGTAAG 85 3.8085527E-6 12.329799 1 GTCCTAT 70 1.0600647E-4 12.249736 1 CTAGCAG 55 0.0029895513 12.131395 3 CGGTTTC 55 0.0031043414 12.070533 13 TGTAGGA 665 0.0 11.898611 2 TCTAAAT 320 0.0 11.847121 18 TCCTACA 540 0.0 11.828259 2 CCCAGTA 65 7.781786E-4 11.7332 2 CTAAATT 275 0.0 11.717879 19 CTGTAGG 700 0.0 11.705302 1 TCTTACA 90 7.1592094E-6 11.651719 2 GACGTGG 245 0.0 11.634509 7 CTACACT 115 6.726805E-8 11.603943 4 CTATACT 75 2.0071949E-4 11.438173 4 >>END_MODULE