FastQCFastQC Report
Thu 26 May 2016
SRR1926472_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926472_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences864033
Sequences flagged as poor quality0
Sequence length25
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG1003.3071046E-815.9151041
CTGTAGG5050.014.9696531
TGTAGGA5250.014.7500082
CCTATCC450.00467899414.5094223
TCCTATA852.343667E-514.3846842
GTAGGAC5300.014.167223
TAGGACC2250.013.7685574
TAATACA1407.2759576E-1213.7346444
GTCCTAC4950.013.6644841
CTAGACT1055.8736987E-813.2259544
TCCTACA5600.013.1003362
TAGGACA1553.6379788E-1213.0946794
TAGACTG1058.75898E-812.8279935
TTAATAC851.3218909E-512.8024323
TAGGACG4650.012.6352164
GTGTAAC958.354267E-512.5645571
AGGACGT4400.012.4803835
GGACGTG4200.012.0831826
CTTTAGG3000.011.9363291
GATACTG707.2046975E-411.8412255
Produced by FastQC (version 0.11.5)