FastQCFastQC Report
Thu 26 May 2016
SRR1926472_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926472_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences864033
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA15670.18135881384160096No Hit
GTCCTACAGTGGACATTTCTAAATT14780.17105828133879145No Hit
GTACATGGGAAGCAGTGGTATCAAC10370.12001856410576912No Hit
CTTTAGGACGTGAAATATGGCGAGG10330.11955561882474396No Hit
GTCCTAAAGTGTGTATTTCTCATTT10280.11897693722346253No Hit
CCCATGTACTCTGCGTTGATACCAC10080.1166622108183368No Hit
GTCCTACAGTGTGCATTTCTCATTT9800.11342159385116077No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGACC400.005154078414.3005334
TCTTATA1204.9112714E-1113.510782
TAACCAA500.001518444713.27680710
TAGACTG1457.2759576E-1212.4924195
TAGGACC2750.012.1337854
TCTAGAC802.7577116E-511.9184983
GTCCTAA3050.011.8766561
AGGACCT5800.011.8349235
GGACCTG5800.011.6705496
TATTATA907.1338964E-611.6563592
GTCCTAG1501.6552804E-1011.4390941
GCCTAAG752.0069143E-411.4390941
TCCAACG1001.9890267E-611.36958118
GGCGAGA4850.011.32985519
TGGCGAG7600.011.21998218
GTCCATA600.00572427911.1213421
GTAGGAC8250.011.0950393
GTCCTAC7700.011.0181741
TGTAGGA8800.010.94592
TAGTACT1053.3160413E-610.8956444
Produced by FastQC (version 0.11.5)