FastQCFastQC Report
Thu 26 May 2016
SRR1926471_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926471_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences981175
Sequences flagged as poor quality0
Sequence length25
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG758.643901E-615.9845481
TGTAGGA5750.015.4266562
TAGGACC3000.014.9762964
GTCCTAC6400.014.7773811
TCCTACA7300.014.2074782
CTGTAGG5950.013.6562661
CCCTATA600.007023855613.6466572
GTTAGAA600.007023855613.6466572
TGTAGTA1101.2184082E-613.6466572
CTAAGAC652.4698547E-413.4443523
GTAGGAC6050.013.3610213
GATTAAT600.00824290413.32045651
GTAGTAT500.00916809313.1082443
TATAAGG853.632481E-412.8439122
GTCTTAG1254.797912E-712.7876381
GGTCAGG1152.6349207E-612.7413051
GTCTAAG1051.4514551E-512.6861491
GTGCTAG1051.4514551E-512.6861491
TGTAGAA2055.456968E-1212.6481212
GTCCTAA2850.012.6193811
Produced by FastQC (version 0.11.5)