FastQCFastQC Report
Thu 26 May 2016
SRR1926471_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926471_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences981175
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA17930.1827400820444875No Hit
GTCCTACAGTGGACATTTCTAAATT17140.17468851122378778No Hit
GTCCTACAGTGTGCATTTCTCATTT13930.14197263485107142No Hit
GTACATGGGAAGCAGTGGTATCAAC12540.12780594695135933No Hit
CTTTAGGACGTGAAATATGGCGAGG12400.12637908629958977No Hit
CCCATGTACTCTGCGTTGATACCAC12300.12535990011975437No Hit
GTCCTAAAGTGTGTATTTCTCATTT11990.12220042296226463No Hit
CTGTAGGACCTGGAATATGGCGAGA11010.1122123983998777No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4950.013.8725144
TAAGTTG655.251461E-513.20556455
TGTAGGA10450.013.1491282
CTGTAGG10500.012.7177751
GTAGGAC10250.012.6583353
GTCCTAC8900.012.324811
AGGACCT6800.012.2022025
GGACCTG6850.012.1125136
TGGCGAA550.003132649512.05679218
TAAGGCT959.903688E-712.0471824
TAGGACG8350.011.9930784
AGGACGT8450.011.7382795
TAGGCTG907.1339127E-611.6567645
GGCGAGG4050.011.46139519
TTAGGAC4500.011.23634053
CCCTATA855.0828632E-511.2261752
GGACGTG8450.011.1733676
GACGTGG4850.011.1647397
TCCTACA10600.011.1626292
GTAGAAC2650.011.1603383
Produced by FastQC (version 0.11.5)