FastQCFastQC Report
Thu 26 May 2016
SRR1926470_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926470_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences883385
Sequences flagged as poor quality0
Sequence length25
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT10130.11467253802136101No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGG654.466407E-516.4321271
CCAACGA705.806214E-915.76966219
GTATTAA708.415198E-515.2584041
GTATAAT550.004560497614.564841
TTATAGT754.185791E-614.310654
GTTCTAT955.044687E-614.0537921
TAGGACC2850.013.9340534
GCTACAC806.5440345E-613.7057763
GTCCTAC7250.013.6273341
TTCTATG956.2358944E-512.9630632
TCCAACG1202.5465852E-1112.74516418
TTTAGAA1952.910383E-1112.6306772
GTAGGAC6800.012.5770653
TGTAGGA5900.012.5236382
GTCCTAA2550.012.0421721
GAAATGT1651.4551915E-1112.0077376
TCCTACA8100.011.9939462
TTGTACC902.7382113E-511.925544
TATAGTT706.804279E-411.9221245
GGACGTG5750.011.7879146
Produced by FastQC (version 0.11.5)