FastQCFastQC Report
Thu 26 May 2016
SRR1926470_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926470_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences883385
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA20780.2352315241938679No Hit
GTCCTACAGTGGACATTTCTAAATT18810.21293094177510372No Hit
GTCCTAAAGTGTGTATTTCTCATTT15900.17998947231388354No Hit
CTTTAGGACGTGAAATATGGCGAGG15680.17749905194224488No Hit
GTCCTACAGTGTGCATTTCTCATTT13640.15440606304159568No Hit
CCCATGTACTCTGCGTTGATACCAC12360.13991634451569812No Hit
CTGTAGGACCTGGAATATGGCGAGA11240.1272378408055378No Hit
GTACATGGGAAGCAGTGGTATCAAC11020.12474742043389914No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTTC351.0100484E-418.95875513
GTATAAG1050.017.2587591
GCCGGTT508.870015E-515.16700611
TAGGACC3850.013.1321154
GGCTAAG701.0502584E-412.2628031
GATATAC2100.012.2628021
TATAAGG1103.569221E-812.1465532
CTAAGAC1103.5863195E-812.1423973
CCCCTAT550.002976896712.1389361
TCCAACG951.0759268E-611.96446118
TTCTATA1052.5574445E-711.8160342
CTTAGAG1156.652772E-811.6144683
CTGCGTT3400.011.43101411
TGCGTTG3400.011.43101412
CCAACGA752.1297409E-411.36623919
CGTTGAT3400.011.15220914
GGACCTG8650.011.1384526
TAATACT951.2973582E-511.0455844
AGGACCT8900.010.9327575
GCCCTAA700.001441796510.90026951
Produced by FastQC (version 0.11.5)