FastQCFastQC Report
Thu 26 May 2016
SRR1926469_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926469_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences819987
Sequences flagged as poor quality0
Sequence length25
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT11280.13756315648906628No Hit
GTCCTAAAGTGTGTATTTCTCATTT8380.10219674214347302No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATTAG300.002501828422.1140961
GTCTAAG604.7454453E-415.4798671
GTATAAG708.871186E-515.1639521
TTTAGGA3900.014.6331422
GATATAC955.3779513E-613.9667981
TGTAGGA6050.013.9248082
GTCCTAA4200.013.9002891
TCCTACA7800.013.7621222
GTAAGAC806.9826983E-613.6196713
TTAGGAC3750.013.36543753
GTCCTAC7300.013.2684571
CTCTTAT700.001523855613.2684571
GATCAAG802.7582556E-413.2684571
GTGTAGG1401.0342774E-813.2684571
GTACTAA600.00845611213.2684571
TAGGACC2500.013.2411944
CTGTAGG5900.013.0435681
TAGGACA2150.012.9133924
TAGGACG6450.012.9133914
GTAGGAC6900.012.7906483
Produced by FastQC (version 0.11.5)