##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926469_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 819987 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.796182134594815 27.0 10.0 31.0 2.0 31.0 2 16.771293935147753 22.0 2.0 30.0 2.0 31.0 3 20.382929241561147 25.0 15.0 31.0 2.0 31.0 4 22.562036959122523 28.0 7.0 35.0 2.0 37.0 5 23.122360476446577 30.0 10.0 35.0 2.0 37.0 6 23.96416406601568 31.0 10.0 35.0 2.0 37.0 7 23.727981053358164 30.0 10.0 35.0 2.0 37.0 8 24.174790575948155 31.0 17.0 35.0 2.0 37.0 9 27.037074978017944 32.0 18.0 37.0 2.0 39.0 10 27.782989242512382 32.0 25.0 38.0 2.0 39.0 11 24.815122678774177 30.0 10.0 37.0 2.0 39.0 12 25.975013018499073 31.0 17.0 37.0 2.0 39.0 13 22.523748547233065 29.0 2.0 37.0 2.0 39.0 14 26.01763564544316 31.0 17.0 38.0 2.0 40.0 15 27.170455141361998 32.0 19.0 38.0 2.0 40.0 16 27.56376381576781 32.0 23.0 39.0 2.0 40.0 17 26.82050934953847 31.0 17.0 38.0 2.0 40.0 18 26.91084248896629 31.0 18.0 38.0 2.0 40.0 19 27.325244180700427 31.0 23.0 39.0 2.0 40.0 20 27.600999771947603 32.0 25.0 39.0 2.0 40.0 21 25.358239825753333 31.0 10.0 38.0 2.0 40.0 22 26.34086150146283 31.0 17.0 38.0 2.0 40.0 23 26.34979700897697 31.0 11.0 39.0 2.0 40.0 24 25.391352545833044 31.0 2.0 39.0 2.0 40.0 25 25.181429705592894 31.0 2.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.105527923562757 1101 2 11.02809480221864 1101 3 7.251083480974454 1101 4 9.05408919608793 1101 5 8.577514678354017 1101 6 8.118496812177177 1101 7 7.613195993979861 1101 8 6.318500314161771 1101 9 6.1676329632442375 1101 10 5.676533040247261 1101 11 8.816255214758673 1101 12 5.889276540802378 1101 13 10.664344414351852 1101 14 8.048450151471734 1101 15 7.305549340322205 1101 16 6.912919356033921 1101 17 2.8443763710562173 1101 18 5.41356857948783 1101 19 5.877628915217915 1101 20 4.048630891767438 1101 21 0.782730022350755 1101 22 4.864513884294066 1101 23 6.232074553822553 1101 24 7.6826577871042225 1101 25 8.219607677850597 1102 1 5.586834037385916 1102 2 10.927917273039327 1102 3 6.813215024764087 1102 4 7.973494666569607 1102 5 7.917183424266174 1102 6 6.951325376910756 1102 7 6.716220643091898 1102 8 7.008312957037539 1102 9 6.039194099763641 1102 10 5.329893253262707 1102 11 8.278200913039296 1102 12 5.6851189629872145 1102 13 9.975065973360373 1102 14 7.55404581837044 1102 15 5.410533708068353 1102 16 5.8471841509067595 1102 17 6.594175678452711 1102 18 6.768518825822408 1102 19 5.34593122456484 1102 20 5.812369835329399 1102 21 4.951857751880233 1102 22 6.188099337761514 1102 23 6.297203156077455 1102 24 7.682275235938004 1102 25 7.531580110685226 1103 1 4.923945756513131 1103 2 9.181268387011691 1103 3 6.118022694963891 1103 4 7.8261491167110115 1103 5 7.089257091293053 1103 6 6.691950305060111 1103 7 6.7085527425101965 1103 8 6.531385635399573 1103 9 5.370178396087187 1103 10 4.677293984414849 1103 11 5.6899683238870224 1103 12 4.529887086593309 1103 13 8.702033531358211 1103 14 6.797786190384329 1103 15 5.795239292875099 1103 16 5.451085928204254 1103 17 3.2416936698536283 1103 18 2.3756854963211076 1103 19 4.328928488492366 1103 20 4.86422875515558 1103 21 7.00752188174021 1103 22 2.500528924131732 1103 23 4.898437837520444 1103 24 6.315032231316568 1103 25 2.220771474812789 1104 1 6.186770995052548 1104 2 10.945353788581407 1104 3 7.54730353444625 1104 4 8.231128318508695 1104 5 8.212545038305809 1104 6 7.587209618714642 1104 7 7.404678975471153 1104 8 7.575673008172959 1104 9 6.597130929786601 1104 10 5.577674957247286 1104 11 8.525899794793023 1104 12 5.331627348250898 1104 13 9.840243074085357 1104 14 7.545499538525817 1104 15 6.912195268176095 1104 16 6.615374470546978 1104 17 6.8020760423346225 1104 18 4.271243433622605 1104 19 5.2319975534792 1104 20 4.436758351491186 1104 21 3.5596506417378713 1104 22 5.676240899467242 1104 23 6.21647681485668 1104 24 7.316252130484354 1104 25 6.032344267562227 1105 1 3.399423839284342 1105 2 9.642587650574992 1105 3 6.383478292817806 1105 4 5.944350768573251 1105 5 1.9114926708918851 1105 6 4.790015711680919 1105 7 5.936462980939368 1105 8 2.805968773937831 1105 9 3.9432432461506366 1105 10 3.7182899994641616 1105 11 7.1183393918092825 1105 12 4.756394800478304 1105 13 5.703369269019333 1105 14 3.9137059570754857 1105 15 4.4983048108073795 1105 16 4.803932162537166 1105 17 5.599504395827047 1105 18 2.804156744567951 1105 19 2.707220525269676 1105 20 1.6818671709707118 1105 21 2.69564940016226 1105 22 4.326849634744168 1105 23 1.5951474704152098 1105 24 4.8800826554132755 1105 25 5.608580642425604 1106 1 6.370746877771559 1106 2 11.05931916334804 1106 3 7.5158153839478885 1106 4 8.916384539114485 1106 5 8.451422421723649 1106 6 7.344792177554073 1106 7 7.10403421567478 1106 8 7.0535910053368305 1106 9 6.018158056626767 1106 10 5.257978157581906 1106 11 7.775274928692905 1106 12 4.992251092142514 1106 13 4.260316323695136 1106 14 5.507757626078487 1106 15 5.5826740431324815 1106 16 5.587926270671442 1106 17 6.053664987560641 1106 18 6.019542267545948 1106 19 5.7901687616896815 1106 20 5.256016469649538 1106 21 5.245167010679221 1106 22 5.769134259053452 1106 23 6.022222522409145 1106 24 6.965123290745929 1106 25 6.260943241251539 1107 1 3.714753966992319 1107 2 -14.146922356326318 1107 3 -5.718421928159033 1107 4 1.5909667656778481 1107 5 4.338193776675276 1107 6 4.721395097216938 1107 7 4.731251254555001 1107 8 5.264537752206568 1107 9 3.379112979966834 1107 10 2.869899893291869 1107 11 5.573734216500238 1107 12 2.7590289882704013 1107 13 4.862661423779553 1107 14 3.7887643484630082 1107 15 3.266981849672778 1107 16 3.1858795012611623 1107 17 3.6303633730392306 1107 18 -1.7368431138090479 1107 19 1.1465130397073544 1107 20 1.5318857168397066 1107 21 -1.793000752813061 1107 22 1.398218184996729 1107 23 -1.4178625128822695 1107 24 1.965609459251933 1107 25 2.6843876125251676 1108 1 5.116017221889511 1108 2 10.532187008441202 1108 3 0.01509741636483497 1108 4 5.93168977752326 1108 5 7.24848817351479 1108 6 7.24978676169712 1108 7 7.217406006479642 1108 8 6.952101666888868 1108 9 5.602132718057959 1108 10 4.600124365282468 1108 11 6.819824461791832 1108 12 2.234086210176205 1108 13 3.5503716729534887 1108 14 5.123851562540132 1108 15 4.885838520797741 1108 16 4.736803691035242 1108 17 -0.2521065654618866 1108 18 3.354027192448136 1108 19 4.660743109098913 1108 20 4.816381726771677 1108 21 4.879700185135569 1108 22 1.8534861200378678 1108 23 4.6789895890228514 1108 24 5.341072620716901 1108 25 5.829993418122143 1109 1 3.2468199060866354 1109 2 -14.146922356326318 1109 3 -5.8836154455790215 1109 4 1.5594445009732816 1109 5 0.6429557039007427 1109 6 2.1661386739575796 1109 7 3.4846164928872305 1109 8 3.69956264801214 1109 9 3.195991652127642 1109 10 2.393626032354579 1109 11 0.7180218615292162 1109 12 1.2088394688030846 1109 13 2.5685418342831987 1109 14 3.324828711962997 1109 15 3.36881902096205 1109 16 3.086153815063888 1109 17 3.516508756996 1109 18 3.7909338122252905 1109 19 2.285118638728033 1109 20 1.09773588914085 1109 21 0.23551184368619715 1109 22 2.6112791071911445 1109 23 3.228005207954496 1109 24 4.470240715929929 1109 25 5.022581858775464 1110 1 2.8745870430487983 1110 2 9.004086690298596 1110 3 6.099448272834259 1110 4 7.366526597847201 1110 5 8.122285226696693 1110 6 3.9073466475649994 1110 7 6.387402438141873 1110 8 7.1931380981136 1110 9 5.133637312463737 1110 10 4.781449243979491 1110 11 2.649449321485065 1110 12 5.296469230904009 1110 13 9.940522240401222 1110 14 7.511490621756764 1110 15 6.601483015918962 1110 16 6.1472226904514535 1110 17 6.8471260368407165 1110 18 6.907239858543324 1110 19 5.6564706740296735 1110 20 5.869715388228023 1110 21 3.6411506529159396 1110 22 5.7662614528434695 1110 23 6.117761151148162 1110 24 7.208177331606347 1110 25 7.721733331415635 1111 1 5.413004328793303 1111 2 9.835883695943284 1111 3 7.74648584059301 1111 4 8.463990077758641 1111 5 8.719175814320497 1111 6 7.668109317003754 1111 7 7.69093340734927 1111 8 8.038842937497957 1111 9 6.418542342525029 1111 10 5.712169001536456 1111 11 7.777668550181559 1111 12 6.921546406243511 1111 13 8.931421532924716 1111 14 6.70210102724198 1111 15 6.2338426716666895 1111 16 6.024098230678835 1111 17 7.315795002734244 1111 18 6.712475625017685 1111 19 5.661177292873955 1111 20 5.128812984760614 1111 21 7.95617057731522 1111 22 7.379109268338652 1111 23 7.430430637765834 1111 24 7.211905199633403 1111 25 7.780462687909704 1112 1 -1.8789172465057966 1112 2 8.197420581129762 1112 3 6.190345849234692 1112 4 -0.5242991884814856 1112 5 4.455743752525716 1112 6 3.510542675050619 1112 7 6.153609853743301 1112 8 6.293871109956516 1112 9 4.953052516798916 1112 10 3.713707877982653 1112 11 -0.2935059354016225 1112 12 4.308412888412551 1112 13 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5 1.5256109197927437 2204 6 4.288587188513265 2204 7 5.353945498421886 2204 8 5.351649663096982 2204 9 3.7742064858095254 2204 10 3.371170934953792 2204 11 5.013496408705429 2204 12 5.062901337419625 2204 13 3.2531354448724663 2204 14 4.427943410443373 2204 15 4.786421042401873 2204 16 4.486966036875387 2204 17 -0.9710192833922839 2204 18 2.760070396133777 2204 19 3.5073590341038354 2204 20 3.969793737158149 2204 21 5.313948974983575 2204 22 4.9568182168866635 2204 23 3.64024778073291 2204 24 5.38666592721394 2204 25 5.4562151335383575 2205 1 5.987981101269593 2205 2 10.529210165647989 2205 3 7.3455322798299 2205 4 7.665333414505188 2205 5 7.9407203539691125 2205 6 7.602381237601431 2205 7 7.632203346050929 2205 8 7.301975672449359 2205 9 6.057854007996603 2205 10 5.369135445088684 2205 11 7.58012950515597 2205 12 6.710270803653184 2205 13 10.158763420682998 2205 14 7.574259433838506 2205 15 6.190551124625262 2205 16 5.845668997201063 2205 17 6.4608426342708505 2205 18 6.587235793278889 2205 19 5.096212303890169 2205 20 5.5988078602899165 2205 21 -0.8541905258131273 2205 22 3.890009231416421 2205 23 5.087378945713976 2205 24 7.175430779670474 2205 25 7.621764526673957 2206 1 4.280241337369006 2206 2 8.83472278303466 2206 3 6.165787665793097 2206 4 1.4769438647285504 2206 5 5.817565871706908 2206 6 6.050309151118981 2206 7 6.10777501357023 2206 8 6.32931054742 2206 9 4.947711974202846 2206 10 4.099282727987408 2206 11 3.556221256936734 2206 12 4.976771355912348 2206 13 8.369845926776694 2206 14 0.3369801249185649 2206 15 2.623850030816584 2206 16 3.5883879324509174 2206 17 4.603678961938183 2206 18 -1.2840411687119833 2206 19 1.141641882458746 2206 20 2.658604450577972 2206 21 3.3944327332375543 2206 22 -2.291412614144985 2206 23 -2.7597080078700564 2206 24 1.2672124371282258 2206 25 3.0438169267145874 2207 1 1.4361736818796942 2207 2 -14.146922356326318 2207 3 -6.3862858955721435 2207 4 -4.623730883015707 2207 5 1.0784438905797842 2207 6 1.6136792850041068 2207 7 2.7506592231105564 2207 8 3.0845785991574424 2207 9 0.585968558114395 2207 10 0.24352300876724442 2207 11 -1.8620329332122232 2207 12 -2.926912815025009 2207 13 0.23418960296211466 2207 14 -6.068885161124236 2207 15 -1.7777793362035155 2207 16 -0.7003764744754761 2207 17 0.6348782984969468 2207 18 0.4389030457388685 2207 19 -0.11040476090421691 2207 20 -0.22283854357434052 2207 21 -4.255857001920525 2207 22 -4.896925742702809 2207 23 -1.653985543354274 2207 24 -0.0012868298411135015 2207 25 0.7321717447768066 2208 1 4.585218730157358 2208 2 6.163624518673682 2208 3 6.016515894180813 2208 4 8.07759729635103 2208 5 2.987443488265935 2208 6 5.752656972895547 2208 7 6.266541105939368 2208 8 6.426817203889524 2208 9 4.474934013490181 2208 10 4.053407968753326 2208 11 6.145529479800999 2208 12 5.476361965333375 2208 13 8.358337404371301 2208 14 2.390190278158993 2208 15 4.01706370702891 2208 16 4.830959153134128 2208 17 5.684443092773215 2208 18 0.22037051268389263 2208 19 2.934254794593347 2208 20 3.9735014877409007 2208 21 6.749869174575647 2208 22 5.657154209019531 2208 23 1.3637217026443338 2208 24 2.851161847959858 2208 25 4.11909301086936 2209 1 2.955128383436538 2209 2 4.894954899991372 2209 3 5.414665243609214 2209 4 0.833079208204218 2209 5 5.040624426141989 2209 6 5.765628919345609 2209 7 6.174730128953808 2209 8 4.948880230363649 2209 9 4.6591374393325395 2209 10 3.548412105335757 2209 11 -0.37834424161897573 2209 12 3.303781395989212 2209 13 2.2426282625241285 2209 14 3.978992361492324 2209 15 4.460584784796662 2209 16 4.030390843116077 2209 17 1.5567406822738192 2209 18 3.6624207533577824 2209 19 4.223263707048222 2209 20 4.279702600857625 2209 21 6.717301579058198 2209 22 5.060413177882346 2209 23 0.7315936190101553 2209 24 -2.9826001857344835 2209 25 0.3738289276864464 2210 1 -2.143114359795913 2210 2 -14.146922356326318 2210 3 -7.822774166177441 2210 4 -2.4383075907206226 2210 5 -0.6097320861110092 2210 6 -1.2151925208739236 2210 7 -1.0970261156338417 2210 8 -21.474224462777144 2210 9 -13.549592612288636 2210 10 -7.4384874751719074 2210 11 -10.862136992161616 2210 12 -5.116895190040456 2210 13 -5.8530199262361755 2210 14 -4.421007638507675 2210 15 -6.089372478718751 2210 16 -5.381432542738146 2210 17 -4.95817153923613 2210 18 -5.006557057409562 2210 19 -10.40629475758734 2210 20 -7.518564322227949 2210 21 -5.649620944193824 2210 22 -10.30984953903343 2210 23 -8.018214629131368 2210 24 -10.717666033660079 2210 25 -8.0645068236322 2211 1 0.424612061602474 2211 2 5.354588217691809 2211 3 1.1151064184758788 2211 4 2.056972453702489 2211 5 1.7201918675458963 2211 6 0.5606331184141773 2211 7 -21.063537019060632 2211 8 -13.363952559453882 2211 9 -8.966969860502772 2211 10 -6.884052693381619 2211 11 -9.503726247792152 2211 12 -4.579904949948599 2211 13 -6.380240846509867 2211 14 -4.56677803125692 2211 15 -5.108795825322549 2211 16 -12.275400599509377 2211 17 -7.958776239427188 2211 18 -5.806410705845813 2211 19 -8.540006440300914 2211 20 -6.498253925149331 2211 21 -4.251438809437445 2211 22 -5.763511151630016 2211 23 -7.903752177013271 2211 24 -22.825921485373474 2211 25 -22.608120530797308 2212 1 -0.5211981025967987 2212 2 -14.146922356326318 2212 3 -7.661776364438701 2212 4 -19.944538120315638 2212 5 -20.414249220067397 2212 6 -21.260363860437785 2212 7 -9.708671933206176 2212 8 -9.430070742417373 2212 9 -4.676083698250078 2212 10 -5.053192518436646 2212 11 -5.150376676508621 2212 12 -2.487019469492189 2212 13 -6.0513136628472886 2212 14 -3.4905088649999065 2212 15 -3.489541997011699 2212 16 -3.630810381309864 2212 17 -3.8771854802371415 2212 18 -2.7903187078121547 2212 19 -3.28771155352603 2212 20 -3.4790784046003367 2212 21 -1.1941790376415788 2212 22 -2.5138248767327163 2212 23 -2.653485387162153 2212 24 -1.5544578713096975 2212 25 -2.1943838177964885 2213 1 -19.25136981150931 2213 2 -14.146922356326318 2213 3 -8.67571735469306 2213 4 -5.756193525721045 2213 5 -20.414249220067397 2213 6 -10.82201926584319 2213 7 -21.063537019060632 2213 8 -10.863582570885253 2213 9 -8.634036284933245 2213 10 -7.493115116832261 2213 11 -6.438437299478281 2213 12 -5.386268242999957 2213 13 -19.779032387295366 2213 14 -12.736886016886054 2213 15 -7.434143359412531 2213 16 -6.485288735379385 2213 17 -5.07599116961417 2213 18 -5.114652009838629 2213 19 -5.99532713108233 2213 20 -5.888688827574416 2213 21 -4.070636878352278 2213 22 -4.803413780845332 2213 23 -4.8791696261844955 2213 24 -3.874063377265365 2213 25 -4.76859350377028 2214 1 -19.25136981150931 2214 2 -14.146922356326318 2214 3 -12.279266180668357 2214 4 -19.944538120315638 2214 5 -20.414249220067397 2214 6 -21.260363860437785 2214 7 -16.721455571096833 2214 8 -15.111328535175334 2214 9 -14.171906225009065 2214 10 -13.936809320085287 2214 11 -14.44647891009342 2214 12 -12.418423220375523 2214 13 -19.779032387295366 2214 14 -17.092500851177356 2214 15 -24.607285251304422 2214 16 -18.623358932273113 2214 17 -24.303187115560117 2214 18 -18.17797978897523 2214 19 -15.256812088890817 2214 20 -13.761674794310382 2214 21 -11.194566039413793 2214 22 -12.245279685211239 2214 23 -12.241159047732742 2214 24 -11.007821937862161 2214 25 -10.758346775141199 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 131814.0 3 4.0 4 553.0 5 2147.0 6 2980.0 7 2891.0 8 2600.0 9 2467.0 10 2310.0 11 2198.0 12 2173.0 13 2348.0 14 3883.0 15 8341.0 16 12435.0 17 13009.0 18 13759.0 19 15654.0 20 18484.0 21 21172.0 22 22409.0 23 24632.0 24 25781.0 25 26488.0 26 22105.0 27 17358.0 28 19149.0 29 22015.0 30 21351.0 31 25018.0 32 32294.0 33 40685.0 34 49485.0 35 56872.0 36 55856.0 37 57957.0 38 39310.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.67009556955808 18.143620977588167 11.849642842106741 26.336640610747015 2 14.16885677994225 20.152538687762956 40.72618943176573 24.95241510052906 3 19.37435277650808 23.751714093138204 28.622579287298795 28.251353843054922 4 11.75669765670189 17.01903136153944 37.454114526755355 33.77015645500332 5 12.729209080269369 37.30626730791116 35.8640291903937 14.100494421425772 6 32.192155557491965 37.4125939057507 17.637639153359036 12.757611383398308 7 28.072803213627584 30.58636892562782 21.9029711023156 19.437856758428996 8 24.830281184308507 37.438830716107304 20.604015789022807 17.12687231056138 9 26.634593904384758 15.061421252253782 19.52747812138739 38.77650672197407 10 14.428925391855735 29.03499147077488 34.881871159838546 21.654211977530842 11 33.78581870967409 21.6260544790499 25.04442630657251 19.543700504703505 12 24.100779027109315 23.878255331377783 31.806131246262147 20.214834395250755 13 28.680409048076072 21.162560746659388 25.093325163600355 25.063705041664186 14 20.526214734755815 21.873958688179368 27.32749063206466 30.272335945000155 15 22.472192231400808 31.786476510909683 22.955783764782687 22.785547492906822 16 21.98057564975908 27.383914649364865 28.306797267132524 22.32871243374353 17 21.559891515928108 28.1084690116328 28.74212944544358 21.589510026995516 18 21.504633755123866 26.757089249292065 30.758034812570546 20.980242183013527 19 23.492284206437375 26.38091302938608 26.73497108896977 23.39183167520678 20 23.67810582001524 27.306367372793787 27.792214462419306 21.22331234477167 21 22.20665083741179 25.8793015064996 26.334286319086154 25.579761337002456 22 23.126993054815667 28.162685306091095 27.133915939235216 21.57640569985802 23 21.969783902163908 27.78923929948264 27.945007483220742 22.29596931513271 24 23.113456393721833 26.65878458897598 28.184140688391924 22.043618328910263 25 22.712157391266132 27.137321828809164 28.016031134484763 22.13448964543994 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 647.0 1 647.0 2 1462.5 3 2278.0 4 2278.0 5 2278.0 6 3310.0 7 4342.0 8 4342.0 9 4342.0 10 5054.0 11 5766.0 12 5766.0 13 5766.0 14 6814.0 15 7862.0 16 7862.0 17 7862.0 18 11345.0 19 14828.0 20 14828.0 21 14828.0 22 21455.0 23 28082.0 24 28082.0 25 28082.0 26 38065.5 27 48049.0 28 48049.0 29 48049.0 30 59499.5 31 70950.0 32 70950.0 33 70950.0 34 79519.5 35 88089.0 36 88089.0 37 88089.0 38 92886.5 39 97684.0 40 97684.0 41 97684.0 42 99795.0 43 101906.0 44 101906.0 45 101906.0 46 101726.0 47 101546.0 48 101546.0 49 101546.0 50 94384.0 51 87222.0 52 87222.0 53 87222.0 54 76028.5 55 64835.0 56 64835.0 57 64835.0 58 54722.5 59 44610.0 60 44610.0 61 44610.0 62 35901.0 63 27192.0 64 27192.0 65 27192.0 66 20687.0 67 14182.0 68 14182.0 69 14182.0 70 10262.0 71 6342.0 72 6342.0 73 6342.0 74 4365.5 75 2389.0 76 2389.0 77 2389.0 78 1627.5 79 866.0 80 866.0 81 866.0 82 554.5 83 243.0 84 243.0 85 243.0 86 155.5 87 68.0 88 68.0 89 68.0 90 37.5 91 7.0 92 7.0 93 7.0 94 4.5 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.099841826760668 2 36.05679114424985 3 5.551917286493566 4 13.555946618665907 5 13.200940990527899 6 14.628280692254878 7 9.135510684925492 8 7.467435459342648 9 0.09853814755599784 10 1.413437042294573 11 5.569722446819279 12 5.790945466208611 13 16.00830257065051 14 1.5502684798661441 15 2.859435576417675 16 2.8611429205584966 17 2.827849709812473 18 1.4638036944488144 19 1.4201444657049442 20 0.27317506253147916 21 4.364093577093295 22 1.5620979356989806 23 0.10012353854390375 24 8.920263370029037 25 0.10134307007306213 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 819987.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 91.4712515471454 #Duplication Level Percentage of deduplicated Percentage of total 1 96.16943836262676 87.96738887615525 2 2.8132683799229956 5.14666359299133 3 0.4920730153999501 1.3503160371363354 4 0.18959654071448673 0.6937053147265363 5 0.08866481879900863 0.4055140971873092 6 0.056072457526034326 0.3077410720338307 7 0.03518216948779419 0.2252709952634628 8 0.029361784236602663 0.21486073214234325 9 0.017835722257402844 0.14683102534187237 >10 0.09420569894615897 1.58265504788715 >50 0.007288782397066458 0.45548721158235395 >100 0.00632479050919123 1.0007423531262365 >500 5.499817412662135E-4 0.3609564430971892 >1k 1.3749543531655338E-4 0.14186720132879077 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1128 0.13756315648906628 No Hit GTCCTAAAGTGTGTATTTCTCATTT 838 0.10219674214347302 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.2195315291583892E-4 0.0 5 0.0 0.0 0.0 1.2195315291583892E-4 0.0 6 0.0 0.0 0.0 1.2195315291583892E-4 0.0 7 0.0 0.0 0.0 1.2195315291583892E-4 0.0 8 0.0 0.0 0.0 1.2195315291583892E-4 0.0 9 0.0 0.0 0.0 1.2195315291583892E-4 0.0 10 0.0 0.0 0.0 1.2195315291583892E-4 0.0 11 0.0 0.0 0.0 1.2195315291583892E-4 0.0 12 0.0 0.0 0.0 1.2195315291583892E-4 0.0 13 0.0 0.0 0.0 1.2195315291583892E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCATTAG 30 0.0025018284 22.114096 1 GTCTAAG 60 4.7454453E-4 15.479867 1 GTATAAG 70 8.871186E-5 15.163952 1 TTTAGGA 390 0.0 14.633142 2 GATATAC 95 5.3779513E-6 13.966798 1 TGTAGGA 605 0.0 13.924808 2 GTCCTAA 420 0.0 13.900289 1 TCCTACA 780 0.0 13.762122 2 GTAAGAC 80 6.9826983E-6 13.619671 3 TTAGGAC 375 0.0 13.3654375 3 GTCCTAC 730 0.0 13.268457 1 CTCTTAT 70 0.0015238556 13.268457 1 GATCAAG 80 2.7582556E-4 13.268457 1 GTGTAGG 140 1.0342774E-8 13.268457 1 GTACTAA 60 0.008456112 13.268457 1 TAGGACC 250 0.0 13.241194 4 CTGTAGG 590 0.0 13.043568 1 TAGGACA 215 0.0 12.913392 4 TAGGACG 645 0.0 12.913391 4 GTAGGAC 690 0.0 12.790648 3 >>END_MODULE