FastQCFastQC Report
Thu 26 May 2016
SRR1926469_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926469_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences819987
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA22820.2782970949539444No Hit
GTCCTACAGTGGACATTTCTAAATT20580.2509795887007965No Hit
CTTTAGGACGTGAAATATGGCGAGG17040.2078081725685895No Hit
GTCCTAAAGTGTGTATTTCTCATTT16680.20341785906361928No Hit
GTCCTACAGTGTGCATTTCTCATTT12820.15634394203810548No Hit
CTGTAGGACCTGGAATATGGCGAGA10940.13341674928992775No Hit
CTGAAGGACCTGGAATATGGCGAGA10630.12963620154953676No Hit
CCCATGTACTCTGCGTTGATACCAC9220.11244080698840347No Hit
GTACATGGGAAGCAGTGGTATCAAC9090.11085541600049757No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCCAT456.8605645E-414.748301514
ACCGTGC400.00527131714.2520188
TAGGACC3900.013.9377274
ATACTAG500.001458002113.3484161
CTAGGAC1750.013.0792113
AGGACCT7900.012.3127415
CTACACG550.002979849212.1371594
TAGTGCT550.002979849212.1371594
TGTAGGA11450.011.9955092
TCTATAC1209.424184E-911.9211563
TTACACA1209.433279E-911.92042354
GGACCTG7900.011.8298896
GTAGGAC11100.011.7707993
GTCCTAG1302.4610927E-911.7348721
AGGACGT12150.011.694775
GGACGTG12300.011.474626
GTCCTAC9650.011.4612611
GTAGTAC751.9981927E-411.4443093
GATATAC2000.011.44151
CTGTAGG11400.011.3745911
Produced by FastQC (version 0.11.5)