FastQCFastQC Report
Thu 26 May 2016
SRR1926468_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926468_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences647765
Sequences flagged as poor quality0
Sequence length25
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTT350.00595598319.0371651
ATAGATC358.5135055E-418.7223033
GTCCTAC3200.015.4082061
TCTAGGA751.2494717E-414.5646122
GTATTAG550.004614840714.5374731
TCCTACA3600.014.4128982
GTCTAAA658.421354E-414.3510941
TTATACT450.005171501514.2912494
TAGGACC1158.712959E-1013.9805714
GTAGGAC2600.013.8617053
CTGAACT703.5097808E-513.7808484
TGTAGGA2900.013.6543262
AGGACGT2350.013.2861455
TTAGACA652.8963873E-413.1919224
TAGGACG2450.013.1246184
AGGACCT1950.012.8092065
AAGTGTG1452.910383E-1112.6383067
CTGCACT600.002379625312.5048444
CTGTAGG2800.012.3741581
ATAAACT705.4126605E-412.2496424
Produced by FastQC (version 0.11.5)