FastQCFastQC Report
Thu 26 May 2016
SRR1926468_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926468_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences647765
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT7070.10914452000339628No Hit
CTGTAGGACGTGGAATATGGCAAGA6760.10435883383634496No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGCGT402.660879E-416.7032766
TCGAACT655.600985E-513.11376719
TAGGACC2400.012.7272974
CTAGACA1154.9349183E-912.4506164
GTTATAG701.0468102E-412.266071
TAGTAGT550.00295738912.1487834
TCTAGAT657.692516E-411.7491873
GATATAC657.732798E-411.74187851
GTAGGAC4150.011.5014643
TGTAGGA4500.011.243322
TGGCGAG3800.011.21572118
CTAGCAC600.005661049411.1372523
CCTCGAA600.006004214811.04956317
GGACCTG3750.010.9446246
GGGTAGG700.0014376910.9031731
GGCGAGA2700.010.87417319
ACTGGAC1158.251882E-710.7913743
TAAGACC803.6016884E-410.7386554
AGGACCT4200.010.6818395
TATTAAT1002.267598E-510.5008352
Produced by FastQC (version 0.11.5)