FastQCFastQC Report
Thu 26 May 2016
SRR1926467_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926467_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences814399
Sequences flagged as poor quality0
Sequence length25
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTTG451.4892648E-624.3575672
ATACCGA250.003524148420.8469856
GTCGAAA250.007331702418.36660411
GGTATAA500.002404399216.0342351
TGTAGGA4350.015.7484262
CCCCTAC801.5459244E-515.0320951
GTAGGAC4350.014.8477483
GTCCTAC4400.014.5765771
TCGCCTT751.5034973E-414.2526521
CTGTAGG4450.014.1125281
GTGTAGG1451.033186E-913.8226161
GTATTAT600.00807278613.3618631
TAGGACC2450.013.1586074
GTCCTAT1051.4046247E-512.7255831
TAAGACT851.5038877E-512.6425834
TCCTACA5150.012.5039452
TCCTAGG1101.7507304E-512.4555742
CTACACT953.436082E-612.4429644
GTGTAGC1307.423132E-712.3340261
GTAAGAC801.0078393E-412.3154523
Produced by FastQC (version 0.11.5)