FastQCFastQC Report
Thu 26 May 2016
SRR1926467_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926467_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences814399
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT13900.1706780091822313No Hit
CTGTAGGACGTGGAATATGGCAAGA13570.1666259413383366No Hit
GTCCTACAGTGTGCATTTCTCATTT10170.12487736355275485No Hit
CTTTAGGACGTGAAATATGGCGAGG9540.11714159766895588No Hit
GTCCTAAAGTGTGTATTTCTCATTT9240.1134578996290516No Hit
CTGTAGGACCTGGAATATGGCGAGA8400.10314354511731964No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCACCG250.005912894419.0644235
GTCATAG350.002125755716.3348791
TAGGACC3650.013.0578234
GGACCTG4700.012.9800326
GGCGAGG2600.012.39096119
CGGTTTC550.003103379412.0715113
TTAAGCT802.764927E-511.9152644
TGTAGGA9750.011.7334012
AGGACCT5150.011.6607645
GGACGTG6050.011.5016776
AGGACGT6400.011.4684425
ACTAGAC752.0062088E-411.4393613
TAATACT752.0073606E-411.4386544
TTGCACC752.0073606E-411.4386544
CTAGACT1251.736771E-811.4386534
CTAGACA1001.8487935E-611.4386534
GTTTAAG752.0142837E-411.4344171
GTCCTAA3000.011.4344171
GGATTAG752.0142837E-411.4344171
GACGTGG3700.011.297927
Produced by FastQC (version 0.11.5)