FastQCFastQC Report
Thu 26 May 2016
SRR1926466_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926466_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences910581
Sequences flagged as poor quality0
Sequence length25
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGCG250.007339225618.3636789
GTATAGG758.482037E-616.0159381
CGGACAG400.002838587215.6415565
ATCTAGG604.5393486E-415.5710511
CTGTAGG5750.015.5517081
TAGGACC2350.015.0836824
TGTAGGA5950.014.9514882
GTCATAT550.0045710114.5599431
TTGGACG450.005101472614.321884
CCTCTAA751.5187095E-414.2363891
GTCCTAC5150.013.9945091
GATATAC1253.1142918E-813.8804791
CCGTTCA400.0066120213.7727599
TCTTATG802.1216596E-413.6863612
CTAGGAC806.6743214E-613.6798573
GTCCTAG1305.208676E-813.3466151
GTCTTAG904.744568E-513.3466141
AACAGAT705.6042438E-513.1131377
GTAGGAC6000.012.9502653
TAGGACA2700.012.7305594
Produced by FastQC (version 0.11.5)