##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926466_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 910581 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 19.4182527419307 26.0 2.0 30.0 2.0 31.0 2 15.279042721075884 10.0 2.0 30.0 2.0 31.0 3 18.582488543029122 16.0 7.0 30.0 2.0 31.0 4 20.639821169121692 27.0 2.0 33.0 2.0 35.0 5 20.766743430842507 27.0 2.0 35.0 2.0 35.0 6 21.646657463751165 28.0 2.0 35.0 2.0 35.0 7 21.500373937079733 28.0 2.0 35.0 2.0 36.0 8 21.665166525547974 28.0 2.0 35.0 2.0 37.0 9 24.243909108580127 30.0 7.0 37.0 2.0 39.0 10 24.912858932923047 31.0 2.0 37.0 2.0 39.0 11 22.190464110276846 28.0 2.0 35.0 2.0 39.0 12 23.224128331252246 30.0 2.0 35.0 2.0 39.0 13 20.090539995892733 25.0 2.0 35.0 2.0 39.0 14 23.19577939798876 29.0 2.0 37.0 2.0 40.0 15 24.208358180106988 30.0 2.0 37.0 2.0 40.0 16 24.54723742313973 31.0 2.0 38.0 2.0 40.0 17 23.872792206294662 30.0 2.0 37.0 2.0 40.0 18 23.935339085704623 30.0 2.0 37.0 2.0 40.0 19 24.273430919380043 30.0 2.0 38.0 2.0 40.0 20 24.49415592901675 31.0 2.0 38.0 2.0 40.0 21 22.46477249140933 30.0 2.0 36.0 2.0 40.0 22 23.32258744691576 30.0 2.0 37.0 2.0 40.0 23 23.30228282821627 30.0 2.0 37.0 2.0 40.0 24 22.44166087366198 30.0 2.0 37.0 2.0 40.0 25 22.234356965497852 30.0 2.0 37.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.118359425555457 1101 2 9.46129921841254 1101 3 6.224092500995187 1101 4 7.8070258507626775 1101 5 7.151027353666798 1101 6 7.078065736628261 1101 7 6.672404916996491 1101 8 5.351252714280701 1101 9 5.49228111471589 1101 10 5.166311294237293 1101 11 7.79323573286247 1101 12 5.279654397351877 1101 13 9.634093370091463 1101 14 7.321934982206969 1101 15 6.527712355347916 1101 16 5.830431570412831 1101 17 2.462911596405249 1101 18 4.695115536268624 1101 19 5.314466804614444 1101 20 3.5153828966248852 1101 21 0.5439776574572441 1101 22 4.113955318703354 1101 23 5.4987457215571105 1101 24 6.663734356846263 1101 25 7.110349192763181 1102 1 5.089577539061487 1102 2 9.808260526170761 1102 3 6.40165989932094 1102 4 7.635809385137474 1102 5 7.375415989880629 1102 6 6.265288068709204 1102 7 6.405423505092198 1102 8 6.491347302640069 1102 9 5.883702247139592 1102 10 5.039393160956692 1102 11 7.5071174809344505 1102 12 5.352914235200405 1102 13 9.246221772965367 1102 14 6.953729194849508 1102 15 5.083656261484624 1102 16 5.559053612466748 1102 17 6.2795123478389065 1102 18 6.261907441878012 1102 19 5.0508503653553305 1102 20 5.425110337136388 1102 21 4.075063281344924 1102 22 5.533642550922721 1102 23 5.816556017516508 1102 24 7.013701399229756 1102 25 6.809273126584287 1103 1 3.88739627106289 1103 2 7.966039576094074 1103 3 5.513173518017382 1103 4 7.027878037791336 1103 5 6.135341079156994 1103 6 5.7729806256607965 1103 7 6.105097224688798 1103 8 5.67992649148389 1103 9 4.818988720835261 1103 10 4.0028820763963076 1103 11 4.614082533121035 1103 12 3.937686071560023 1103 13 7.7133004375338245 1103 14 6.123637201556246 1103 15 5.159521232745028 1103 16 4.893871561793997 1103 17 2.8008560049378914 1103 18 1.677673174708616 1103 19 3.6245131308372436 1103 20 4.039779578374507 1103 21 6.106162531406824 1103 22 1.9756930907352448 1103 23 4.069474012873638 1103 24 5.4583583602937935 1103 25 1.592222711401405 1104 1 5.034519653330232 1104 2 9.440542587253864 1104 3 6.213073977931355 1104 4 6.739203537997799 1104 5 6.8261963029901835 1104 6 6.465713209342738 1104 7 6.344702545978869 1104 8 6.393704811215887 1104 9 5.269664813765189 1104 10 4.299430679638107 1104 11 7.318347411228775 1104 12 4.6140599248173935 1104 13 8.500828761906625 1104 14 6.216558057493732 1104 15 5.638857692673852 1104 16 5.236597607098968 1104 17 5.42439605961275 1104 18 3.1963124992715883 1104 19 3.8757798640289707 1104 20 3.1947461253978133 1104 21 2.2516061489402333 1104 22 4.381946171713352 1104 23 4.79826873630029 1104 24 5.524959464433845 1104 25 4.693520940181898 1105 1 3.2152324712089886 1105 2 8.983187898837803 1105 3 6.067979305247846 1105 4 5.339874506172311 1105 5 2.2984945206461624 1105 6 4.9416679880353875 1105 7 5.84493462306278 1105 8 2.6693421451750083 1105 9 3.62158067694228 1105 10 3.5487221760384173 1105 11 6.687200710973791 1105 12 3.690583102792779 1105 13 4.6174579729682605 1105 14 3.6954809484358613 1105 15 4.176101973859485 1105 16 4.361122627323397 1105 17 4.9504663181063115 1105 18 2.456184954655118 1105 19 2.1187663668408376 1105 20 1.429250939151462 1105 21 2.397573654955682 1105 22 3.532763949097351 1105 23 0.9641928760286547 1105 24 3.8439407358331295 1105 25 4.677969580505522 1106 1 6.027195118641622 1106 2 10.441958976112145 1106 3 7.405750896363259 1106 4 8.738187968215119 1106 5 8.092816873444935 1106 6 7.510280011916539 1106 7 7.495599827715122 1106 8 6.771598892454204 1106 9 5.959378375456414 1106 10 5.384397461706762 1106 11 7.694911887195172 1106 12 5.253746987241431 1106 13 4.356751862768931 1106 14 5.469071653252556 1106 15 5.449593589231046 1106 16 5.609297186138026 1106 17 6.061228143867908 1106 18 5.837510807032018 1106 19 5.941116198863458 1106 20 5.319738708428513 1106 21 5.416565600823176 1106 22 5.813401871518028 1106 23 6.23249505255502 1106 24 7.010109581874239 1106 25 5.886750448054379 1107 1 3.768773923338376 1107 2 -12.756649499536179 1107 3 -4.892231964013488 1107 4 1.6664966431282089 1107 5 4.056830322627661 1107 6 4.578152491978173 1107 7 4.288825776838404 1107 8 4.868242303638709 1107 9 3.346001955278833 1107 10 2.892728298285874 1107 11 5.562533978476505 1107 12 2.9870912587361076 1107 13 4.244214827581342 1107 14 3.872908570022659 1107 15 3.78245016140151 1107 16 3.5505925497843336 1107 17 4.2344172837004095 1107 18 -0.8101187031761405 1107 19 1.7704095460835028 1107 20 2.043062672988871 1107 21 -1.1084804107902961 1107 22 1.7704089527885678 1107 23 -1.2867275770740818 1107 24 1.9613919623986042 1107 25 2.7652797884832516 1108 1 5.368567325763355 1108 2 10.304161311274632 1108 3 0.4550428569455427 1108 4 6.070550389287217 1108 5 7.44001494265439 1108 6 7.613443422005943 1108 7 7.632596752188327 1108 8 7.215022953050941 1108 9 5.80317067560545 1108 10 4.9594553123813085 1108 11 6.844464234090971 1108 12 2.798162240859721 1108 13 3.286414922413016 1108 14 4.956934667206653 1108 15 4.936294538930099 1108 16 4.830732395713653 1108 17 0.5926885761822298 1108 18 3.8451281362812253 1108 19 4.917158482306121 1108 20 5.299098955340941 1108 21 4.904986918466506 1108 22 1.9551305848786207 1108 23 4.825086047897436 1108 24 5.39203398514589 1108 25 5.900929108343096 1109 1 2.9990748981387796 1109 2 -12.756649499536179 1109 3 -5.133932944546142 1109 4 1.5004469891329926 1109 5 0.3416115259536383 1109 6 2.0227208697204944 1109 7 3.113307565808924 1109 8 3.2845722008085723 1109 9 3.1055724189220584 1109 10 2.3436913157405677 1109 11 0.6450146401051633 1109 12 1.4041950092177764 1109 13 2.142574131177053 1109 14 3.187086645452659 1109 15 3.2871338846868525 1109 16 3.043217058960707 1109 17 3.6403527067561434 1109 18 3.6149066938615633 1109 19 2.2611981440077926 1109 20 1.0643818540418835 1109 21 0.658785594120328 1109 22 2.6329409180256746 1109 23 2.9966524103786654 1109 24 4.086910651115314 1109 25 4.2499046288561 1110 1 3.3089150631360447 1110 2 8.902979419209823 1110 3 6.291929254772441 1110 4 7.8443320666197565 1110 5 8.407673842175093 1110 6 3.8821358188173427 1110 7 6.553446060753565 1110 8 7.272883946132662 1110 9 5.4156410241602515 1110 10 5.313536797460877 1110 11 3.3381173707383525 1110 12 6.077482784575658 1110 13 10.022222707165138 1110 14 7.964894773535281 1110 15 7.176601326261839 1110 16 6.771057339397522 1110 17 7.33896848896886 1110 18 7.264727721907683 1110 19 6.1918758798642095 1110 20 6.515445645775927 1110 21 4.595304002257677 1110 22 6.6929540391128235 1110 23 6.989382563921399 1110 24 7.880681549129346 1110 25 8.19886855887372 1111 1 5.1643860452461965 1111 2 9.248439558987995 1111 3 7.394649361437544 1111 4 8.01710120797544 1111 5 8.440183744349909 1111 6 7.666517488438789 1111 7 7.787716132498545 1111 8 7.779394229254276 1111 9 6.29307383344754 1111 10 5.58517506405526 1111 11 7.277077972048222 1111 12 6.607277219822027 1111 13 8.269961446035875 1111 14 6.322434101408376 1111 15 5.769349662061558 1111 16 5.687169679631854 1111 17 6.968069161486479 1111 18 6.722606238950167 1111 19 5.697775671320258 1111 20 5.41385128574213 1111 21 7.970117802299679 1111 22 7.252568550255518 1111 23 7.207054588262924 1111 24 6.695766065595528 1111 25 7.494206111925443 1112 1 -0.9969070737801431 1112 2 7.627227659772844 1112 3 5.868384789001485 1112 4 0.5999302751930138 1112 5 4.061544603531459 1112 6 3.0951876422338493 1112 7 5.592473571949981 1112 8 5.944029179657935 1112 9 4.551917415714954 1112 10 3.5848099900392505 1112 11 0.36562856715549685 1112 12 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2.9949607377159424 2204 4 5.6190050922223165 2204 5 0.6354720108557004 2204 6 3.486444145193353 2204 7 4.636761577759497 2204 8 4.753648881689312 2204 9 3.396853259020453 2204 10 2.956257921971723 2204 11 4.7116240340447995 2204 12 4.693525202961947 2204 13 2.585839736150213 2204 14 3.9972034391182376 2204 15 4.046491175573532 2204 16 4.032113714238719 2204 17 -1.0720364673542662 2204 18 2.4387954303218855 2204 19 3.255007787174719 2204 20 3.5254657871838155 2204 21 4.922422920967847 2204 22 4.425472638761434 2204 23 3.389364488809356 2204 24 4.953126120444622 2204 25 5.032921512581922 2205 1 5.501021208217239 2205 2 9.53211728923061 2205 3 6.590240242142929 2205 4 7.267984336721163 2205 5 7.300355802995249 2205 6 7.131667690230213 2205 7 7.265048384639957 2205 8 6.793407781435768 2205 9 5.944967317402092 2205 10 5.239540842466841 2205 11 7.502576392203132 2205 12 6.532493225190706 2205 13 9.475241111239203 2205 14 7.148919109191095 2205 15 6.062236164871724 2205 16 5.79816661314787 2205 17 6.288430521924173 2205 18 6.218253509406598 2205 19 4.740687805835446 2205 20 5.131713787955775 2205 21 -0.926840413360825 2205 22 3.691702571450609 2205 23 4.840931313742704 2205 24 6.42154876466067 2205 25 6.767400474814465 2206 1 4.083146812081974 2206 2 8.207325655743324 2206 3 5.674908713767923 2206 4 1.9238955752410973 2206 5 5.303599419282342 2206 6 5.851994706209403 2206 7 5.9635778744737955 2206 8 6.09979945739266 2206 9 4.81703824164693 2206 10 3.9293906062296458 2206 11 3.7208754844152665 2206 12 4.862943933902283 2206 13 7.956517322950198 2206 14 0.36547694748806236 2206 15 2.481060818478987 2206 16 3.2107429257241833 2206 17 4.4421758104520706 2206 18 -0.9835195967143342 2206 19 1.0106405583968936 2206 20 2.630476860631891 2206 21 3.4727879775719117 2206 22 -1.138477393511966 2206 23 -2.345297915964789 2206 24 1.4017101491698796 2206 25 2.8394979425641047 2207 1 1.3891306186480783 2207 2 -12.756649499536179 2207 3 -5.571564223232965 2207 4 -3.8773965720526 2207 5 1.2822152134260882 2207 6 1.7147775367329174 2207 7 2.8183527191942943 2207 8 3.0087945771797067 2207 9 0.5712973365892537 2207 10 0.43606207916664985 2207 11 -1.1695193691783459 2207 12 -2.152095995112802 2207 13 0.47469057051009145 2207 14 -4.862467817374405 2207 15 -1.3020691726478972 2207 16 -0.23747135999010283 2207 17 0.6240968397333475 2207 18 0.7085999685030586 2207 19 0.2439147376338049 2207 20 -0.0682088092882509 2207 21 -3.219248402197387 2207 22 -3.9862147493238567 2207 23 -1.246168590321485 2207 24 0.38178446150351064 2207 25 0.9406736302304779 2208 1 5.316462881787288 2208 2 6.584082354093097 2208 3 6.620230581071478 2208 4 8.393734278307939 2208 5 4.278364963067997 2208 6 6.686041064639582 2208 7 6.961587465102255 2208 8 6.754277878478629 2208 9 5.39142820837408 2208 10 4.965179597499716 2208 11 6.81954047191466 2208 12 6.400953599049998 2208 13 8.601327615550066 2208 14 3.2727925030105 2208 15 4.833619250891882 2208 16 5.3643636301547275 2208 17 6.112507831004482 2208 18 1.2159136843313192 2208 19 3.627758377069185 2208 20 4.588067205916872 2208 21 7.045251248892804 2208 22 6.148213467073681 2208 23 2.2303186377431032 2208 24 3.7128176171172846 2208 25 4.716641395861025 2209 1 2.9375589252427616 2209 2 4.511017792521356 2209 3 4.742150662346031 2209 4 0.6538207225575512 2209 5 4.619945427462923 2209 6 5.039854354514439 2209 7 5.464696974532451 2209 8 3.914495365937988 2209 9 3.9649202641843075 2209 10 2.962105540641293 2209 11 -0.4159887824596069 2209 12 2.783766342317481 2209 13 1.9076393363203579 2209 14 3.3546429246710083 2209 15 3.729572893184063 2209 16 3.320472220819333 2209 17 0.9661010898386451 2209 18 3.062188707000331 2209 19 3.7053830022379586 2209 20 3.7563303637918644 2209 21 5.73778628785124 2209 22 4.435202973487595 2209 23 0.03361126252021407 2209 24 -3.199836625017401 2209 25 -0.15692723000104536 2210 1 -2.8345158545042892 2210 2 -12.756649499536179 2210 3 -7.405774315036677 2210 4 -2.8049793770192473 2210 5 -1.4306505153897113 2210 6 -1.8654991127618317 2210 7 -1.8514375027197154 2210 8 -19.14554215751417 2210 9 -12.485102504900741 2210 10 -7.60234068485158 2210 11 -9.644466532509696 2210 12 -5.200623172974236 2210 13 -5.662496124556283 2210 14 -4.562680402408418 2210 15 -6.22249302179921 2210 16 -5.717135021571238 2210 17 -5.1936480252029185 2210 18 -5.244379111478448 2210 19 -10.22077715854214 2210 20 -7.547070426750768 2210 21 -5.841857542941064 2210 22 -9.796643386798264 2210 23 -8.24839144548297 2210 24 -10.18444836657918 2210 25 -8.195656532902738 2211 1 -0.3185055962152248 2211 2 3.9955517583254565 2211 3 0.1771041120256669 2211 4 0.82909890349611 2211 5 0.13869063378291102 2211 6 -0.8171447132991716 2211 7 -18.97182585223428 2211 8 -12.783906937388386 2211 9 -9.281231222003996 2211 10 -7.744924796715779 2211 11 -9.37122967594256 2211 12 -5.636661519435737 2211 13 -6.859955976788072 2211 14 -5.530604930710304 2211 15 -6.000504867680629 2211 16 -11.79222203384455 2211 17 -8.234717818205299 2211 18 -6.6677533558455515 2211 19 -9.177417568873702 2211 20 -7.349409072412367 2211 21 -5.199273431076865 2211 22 -6.802554115258911 2211 23 -8.61656632488701 2211 24 -19.973445130333225 2211 25 -19.771061063647075 2212 1 -0.2522442214787084 2212 2 -12.756649499536179 2212 3 -6.816927900038838 2212 4 -18.089769021032193 2212 5 -18.136152133512688 2212 6 -19.00142144285892 2212 7 -8.657111955776513 2212 8 -8.292681122091828 2212 9 -4.2664522046441355 2212 10 -4.378747393668807 2212 11 -4.762350098200198 2212 12 -1.8914470488858086 2212 13 -4.761103304260525 2212 14 -2.77775760495156 2212 15 -2.7712518729759843 2212 16 -2.7690741237113947 2212 17 -2.9134455614232984 2212 18 -1.954510677662764 2212 19 -2.4459590320375497 2212 20 -2.419000181695523 2212 21 -0.43116382231638894 2212 22 -1.7887921835673097 2212 23 -1.9285057281739029 2212 24 -0.850829326518511 2212 25 -1.5864561590149222 2213 1 -16.935486728290698 2213 2 -12.756649499536179 2213 3 -8.185339733437047 2213 4 -5.682626163889337 2213 5 -18.136152133512688 2213 6 -10.009992871430349 2213 7 -18.97182585223428 2213 8 -10.003399300371314 2213 9 -8.088396540961767 2213 10 -7.166106288180291 2213 11 -6.316049981423243 2213 12 -5.224136811439333 2213 13 -17.483855347857254 2213 14 -11.506013735741751 2213 15 -6.951609988974429 2213 16 -6.127114217132959 2213 17 -4.822637854144201 2213 18 -4.7469357457826575 2213 19 -5.6273097521621125 2213 20 -5.5978771766351905 2213 21 -3.702615749136161 2213 22 -4.637949443201407 2213 23 -4.442316549505161 2213 24 -3.607016558904654 2213 25 -4.231061063647076 2214 1 -16.935486728290698 2214 2 -12.756649499536179 2214 3 -11.24099090957015 2214 4 -18.089769021032193 2214 5 -18.136152133512688 2214 6 -19.00142144285892 2214 7 -14.967006575125845 2214 8 -13.647550189642686 2214 9 -12.587406867984143 2214 10 -12.58382000017111 2214 11 -13.166001214928693 2214 12 -11.168221722512195 2214 13 -17.483855347857254 2214 14 -15.194808916464641 2214 15 -21.734467131831572 2214 16 -16.59350262791896 2214 17 -21.44478071128706 2214 18 -16.108020083132054 2214 19 -13.494360813550523 2214 20 -12.28084045833341 2214 21 -9.849133247701854 2214 22 -10.861446287722348 2214 23 -10.77986101307372 2214 24 -9.763806576116357 2214 25 -9.478691585735428 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 209535.0 3 12.0 4 1121.0 5 3726.0 6 4644.0 7 4511.0 8 3969.0 9 3841.0 10 3501.0 11 3358.0 12 3202.0 13 3312.0 14 4907.0 15 9219.0 16 13177.0 17 14150.0 18 15040.0 19 17294.0 20 20675.0 21 23999.0 22 25288.0 23 27107.0 24 27969.0 25 27859.0 26 23518.0 27 20034.0 28 21297.0 29 23876.0 30 22980.0 31 26743.0 32 33515.0 33 40444.0 34 47479.0 35 52772.0 36 49777.0 37 48322.0 38 28408.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.61425001770377 17.680249673744804 12.089272526783274 26.616227781768153 2 14.242761923161845 19.605424762640762 40.10370666813866 26.048106646058734 3 20.023330769427396 23.180072176663188 28.066520578249865 28.73007647565955 4 11.546221628363181 15.952955176214676 37.45303972060938 35.04778347481276 5 12.76767963826853 36.743046577747776 35.59829943580954 14.890974348174153 6 32.59638216953995 36.36492338976943 18.08001441469269 12.958680025997928 7 28.39662058669562 30.131284293386585 22.57837795916785 18.893717160749944 8 26.063848764720017 36.92246765818886 19.707038729303978 17.30664484778715 9 26.369539213875477 15.207635205854157 19.366605979049577 39.05621960122079 10 14.0349411520956 29.465166897246192 34.63905950288392 21.860832447774296 11 34.17907152402533 21.70925570855848 24.09900644936378 20.012666318052407 12 24.06537321012185 24.090181672486366 31.36977653748407 20.474668579907707 13 28.713542075387792 20.623348766643122 24.65824374166209 26.004865416306995 14 20.312172310548085 21.98191042671581 27.401196749766854 30.30472051296925 15 22.709484983927304 31.153220362746616 22.921714707621614 23.21557994570447 16 22.142410180335233 27.998411302833514 27.474729644251777 22.384448872579473 17 21.541024817901082 27.95958783023091 28.71036437869341 21.789022973174603 18 21.45418431052144 27.06339749584517 30.263200427130833 21.219217766502556 19 23.03241359642087 26.549159581484055 26.80104005133415 23.617386770760923 20 23.39521231779108 27.408406804301976 27.74388598553893 21.452494892368012 21 22.105269191308384 25.613554425765333 26.51011366046301 25.771062722463274 22 22.531043616713845 27.91755324193658 27.59386726085341 21.957535880496163 23 21.866984474962152 27.69824630069605 27.972443909877597 22.4623253144642 24 22.832900269743654 26.846318597091408 28.129805526060526 22.19097560710442 25 22.50322963679642 27.18985866559653 28.01565617973822 22.291255517868823 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 783.0 1 783.0 2 1696.5 3 2610.0 4 2610.0 5 2610.0 6 3794.0 7 4978.0 8 4978.0 9 4978.0 10 5803.0 11 6628.0 12 6628.0 13 6628.0 14 7884.5 15 9141.0 16 9141.0 17 9141.0 18 12882.0 19 16623.0 20 16623.0 21 16623.0 22 23524.0 23 30425.0 24 30425.0 25 30425.0 26 40577.0 27 50729.0 28 50729.0 29 50729.0 30 62436.0 31 74143.0 32 74143.0 33 74143.0 34 84115.0 35 94087.0 36 94087.0 37 94087.0 38 101134.0 39 108181.0 40 108181.0 41 108181.0 42 111371.5 43 114562.0 44 114562.0 45 114562.0 46 114090.0 47 113618.0 48 113618.0 49 113618.0 50 106025.0 51 98432.0 52 98432.0 53 98432.0 54 86568.0 55 74704.0 56 74704.0 57 74704.0 58 62959.0 59 51214.0 60 51214.0 61 51214.0 62 41131.0 63 31048.0 64 31048.0 65 31048.0 66 23831.0 67 16614.0 68 16614.0 69 16614.0 70 12117.5 71 7621.0 72 7621.0 73 7621.0 74 5269.5 75 2918.0 76 2918.0 77 2918.0 78 1989.5 79 1061.0 80 1061.0 81 1061.0 82 705.5 83 350.0 84 350.0 85 350.0 86 212.0 87 74.0 88 74.0 89 74.0 90 54.0 91 34.0 92 34.0 93 34.0 94 18.0 95 2.0 96 2.0 97 2.0 98 1.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.155227266986683 2 36.336031610587085 3 5.481665002893757 4 13.618997101850358 5 13.438892311612035 6 14.671511924804054 7 9.373026671981954 8 7.656540165015523 9 0.1103690940179951 10 1.4104181835553344 11 5.494404122203297 12 5.710969150465472 13 16.033828950966473 14 1.5542823757578952 15 2.873110684277401 16 2.947349000253684 17 2.844008385854745 18 1.475102160049463 19 1.4203019830196326 20 0.2883873043694081 21 4.200175492350489 22 1.575038354632921 23 0.11179675394061593 24 9.08848306740422 25 0.11377351383347555 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 910581.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 94.37215681763477 #Duplication Level Percentage of deduplicated Percentage of total 1 97.29257268469412 91.81709926591083 2 2.0738963246328663 3.9143613834353843 3 0.3112091400279572 0.881084332973989 4 0.11271627476280441 0.42549111831259945 5 0.05598480708167029 0.26417034966582087 6 0.03252732929716161 0.18418045327743487 7 0.021322478257928008 0.14085737833284528 8 0.017740308033031626 0.13393529053491252 9 0.011949735264933878 0.10149500613164043 >10 0.061767538404548634 1.0370877180430464 >50 0.004276400393526075 0.2870731236053013 >100 0.00356204073318318 0.5539873629896764 >500 4.749384162329687E-4 0.25917721678647904 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.0981999404775633E-4 0.0 5 0.0 0.0 0.0 1.0981999404775633E-4 0.0 6 0.0 0.0 0.0 1.0981999404775633E-4 0.0 7 1.0981999404775633E-4 0.0 0.0 1.0981999404775633E-4 0.0 8 1.0981999404775633E-4 0.0 0.0 1.0981999404775633E-4 0.0 9 1.0981999404775633E-4 0.0 0.0 1.0981999404775633E-4 0.0 10 1.0981999404775633E-4 0.0 0.0 1.0981999404775633E-4 0.0 11 1.0981999404775633E-4 0.0 0.0 1.0981999404775633E-4 0.0 12 1.0981999404775633E-4 0.0 0.0 1.0981999404775633E-4 0.0 13 1.0981999404775633E-4 0.0 0.0 2.1963998809551265E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAATGCG 25 0.0073392256 18.363678 9 GTATAGG 75 8.482037E-6 16.015938 1 CGGACAG 40 0.0028385872 15.641556 5 ATCTAGG 60 4.5393486E-4 15.571051 1 CTGTAGG 575 0.0 15.551708 1 TAGGACC 235 0.0 15.083682 4 TGTAGGA 595 0.0 14.951488 2 GTCATAT 55 0.00457101 14.559943 1 TTGGACG 45 0.0051014726 14.32188 4 CCTCTAA 75 1.5187095E-4 14.236389 1 GTCCTAC 515 0.0 13.994509 1 GATATAC 125 3.1142918E-8 13.880479 1 CCGTTCA 40 0.00661202 13.772759 9 TCTTATG 80 2.1216596E-4 13.686361 2 CTAGGAC 80 6.6743214E-6 13.679857 3 GTCCTAG 130 5.208676E-8 13.346615 1 GTCTTAG 90 4.744568E-5 13.346614 1 AACAGAT 70 5.6042438E-5 13.113137 7 GTAGGAC 600 0.0 12.950265 3 TAGGACA 270 0.0 12.730559 4 >>END_MODULE