FastQCFastQC Report
Thu 26 May 2016
SRR1926466_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926466_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences910581
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA17770.19515012942286297No Hit
GTCCTACAGTGGACATTTCTAAATT17070.18746272983952006No Hit
GTCCTACAGTGTGCATTTCTCATTT12400.13617679261921783No Hit
CTTTAGGACGTGAAATATGGCGAGG12040.1322232728334986No Hit
GTCCTAAAGTGTGTATTTCTCATTT11850.13013669294659125No Hit
CCCATGTACTCTGCGTTGATACCAC10090.11080837399418612No Hit
CTGTAGGACCTGGAATATGGCGAGA10050.1103690940179951No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC3250.014.3759024
AAAGGCG957.026756E-813.0479465
GTACTAA751.4261537E-512.7098711
GACAGTA604.0896155E-412.6664837
AAGGCGT1001.3753743E-712.3934946
AATCCCG854.059317E-612.2608619
TGTAGGA9100.012.1585862
GTCCTAT1103.6294296E-812.1321511
ACTGTTC2150.011.9326888
TTAGGAC4050.011.7729523
CTGTAGG9850.011.7098561
GTAGGAC9550.011.682963
TCTATAC907.1428367E-611.6552233
TAGAGGT1156.706614E-811.6078714
GTCCTAC9500.011.4388841
GTGTAGG1251.7373168E-811.4388831
GGCGAGG3900.011.41774719
AGGACGT7650.011.3423325
TAGGACG7850.011.2962874
GGACGTG7600.011.2896196
Produced by FastQC (version 0.11.5)