FastQCFastQC Report
Thu 26 May 2016
SRR1926465_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926465_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences942989
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT15090.16002307556079656No Hit
CTGTAGGACGTGGAATATGGCAAGA11520.12216473362891826No Hit
GTCCTAAAGTGTGTATTTCTCATTT11410.12099823009600323No Hit
GTCCTACAGTGTGCATTTCTCATTT10380.11007551519688989No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTAGG509.311708E-519.1582052
TAGGACC3500.016.2602714
GTCCTAC10000.014.8147511
GTGTAAG1005.340098E-714.6812861
TGTAGGA8450.014.4131892
CTGTAGG8050.014.2585041
GTGATAC751.5188665E-414.2363981
TCCTACA11150.014.1139882
GTATAGC955.063981E-614.0490781
GTCCTAA4850.013.7594051
TCGTCAA600.006896037613.6844332
TACTAGA700.001207696413.6844312
ATAATAC652.4438163E-413.4610453
GTAGGAC9750.013.4610443
GTATTAG600.00813613913.3466241
GTCCTAG904.745175E-513.3466231
GGACCTG6150.013.2051386
ATTAACC500.00909280213.1245193
AGGACCT6750.012.9624885
CAAGACT756.6038076E-512.8854984
Produced by FastQC (version 0.11.5)